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201817·238·٭(17230002710048)[]3[]UASB(DGGE)[]TQ[]A[]1007-1865(2018)17-0238-03StudyontheCharacteristicsofAnaerobicGranularSludgeTreatingDifferentWastewatersandtheDynamicChangesofMicroorganismsZhouTiean1,WangNing2,LiuYonghong2*(1.HanzhongEnvironmentalMonitoringCenterStation,Hanzhong7230002.CollegeofEnvironmentandChemicalEngineering,Xi’anPolytechnicUniversity,Xi’an710048,China)Abstract:Asthemainpartofbio-treatment,thecharacteristicsofanaerobicgranularsludgedeterminetheefficiencyofwastewatertreatment.Thecharacteristicsofanaerobicgranularsludgeandthedynamicchangesofmicroorganismsinthesludgewereinvestigatedunderthesamecandition,duringtreatingdifferenteffluentsatdifferentstages.Themacroscopicandmicroscopicchanginglawinsidethegranularsludgewasexpectedasthetheoreticalguidancefortheproblemsarisinginactualindustrialwastewatertreatmentandreactoroperation.Threetypesofwastewatersweresimulatedglucosewastewater,practicalpapermakingwastewaterandpracticalprintinganddyeingwastewater,respectively.Thepropertiesofanaerobicgranularsludgefocusedinthispaperwerespecificmethanogenicactivity,sludgemechanicalstrengthandmicrobialfloradiversity.Keywords:UASBreactorsanaerobicgranularsludgeDenaturingGradientGelElectrophoresisarchaeabacteria[1-2][3][4-5]Britz[6][7-8]PCR-DGGE(R1)(R2)(R3)311.1UASBR1R2R32.5L0.6m0.06m1UASB1UASB1.2COD2000~2500mg/LpH6~911COD/mg·L-1pHSS/mg·L-1ALK/mg·L-1500~12006.5~7.81501800500~100010.2~12.010036001.3R1R2R3R1-R3221~3940~4748~63R2R320%64~79R2R340%80~92R2R360%93~108R2R380%108~136R2R3100%1.43[]2018-08-30[](2017slkj-9)(2011KTZB03-03-01)[]19907*20181745379·239·3COD[9][10](SS)[11]PCR-DGGE[12][13]22.12.1.1223CODR1-R3VFANaHCO3COD98%47COD90%2.1.248R2R3R10.13L·h-122.8hCOD2500mg·L-1136COD22R1-R3COD2R195%R2SSCODCODCOD[14]COD93%R320%40%R395%COD89%(SMA)44(gCOD·gVSS-1·d-1)2447637992108134R10.220.420.550.650.490.490.750.63R20.220.250.360.380.480.430.510.37R30.220.350.530.680.570.630.370.2947R2R347R1SMA0.60gCOD·gVSS-1·d-1R2SMA60%0.37gCOD·gVSS-1·d-180%R3SMA0.60gCOD·gVSS-1·d-1R3SMACOD89%(2)SMASSSSVSSSMA[15-16]55(kPa)4792135R189.6677.5984.4885.96R289.6686.2189.6694.96R389.6691.3877.5974.16547R1R2R392R1R2R3R2R20.9~2.0mm57.8%R374.16kPa[13,17]2.22.2.1DNAPCROMEGADNAPCRPCR-DGGE177bp406bpDGGE[18-19]2.2.2(DGGE)8%40%~60%30%~45%25μL60℃160V30min80V16h35(1)3DNADGGEQuantityOneDGGEShannon-Weiner442341367822521R2R360%3R1R2(5#8#9)R3()R2R3100%R2R36(X7X10X13)X4X8X96-12345678910111213(X)1.821.851.991.681.961.752.051.641.662.022.092.142.43(G)1.691.701.712.311.891.802.622.642.632.542.201.552.53201817·240·(2)5DNADGGE56G2G3G420%G5G6G7100%66R32.53100%3(1)3UASBCOD2000~2500mg·L-13.16L·d-12.5kgCOD·m-3·d-1COD95%COD89%(0.3gCOD·gVSS-1·d-1)(2)(3)SS(4)()3[1]ASBR[D]2015[2][D]2016[3][J]201636(4)1136-1144[4]TyupaDVKalenovSVSkladnevDAetalToxicinfluenceofsilveranduraniumsaltsonactivatedsludgeofwastewatertreatmentplantsandsyntheticactivatedsludgeassociatesmodeledonitspurecultures[J]BioprocessBiosystEng201538(1)125-135[5][J]()201141(3)873-878[6]BritzTJVanSchalkwykCHungYT(2004)TreatmentofdairyprocessingwastewatersChapter13InHandbookofIndustrialandHazardousWastesTreatment(editedbyL.KWangY-THungH.HLoCYapijakis)NewYorkMarcelDekkerInc[7][J]201117(3)427-434[8][J]201420(4)551-557[9]LagunaAOuattaraAGonzalezRetalAsimpleandlowcosttechniquefordeterminingthegranulationofupflowanaerobicsludgeblanketreactor[J]WatSciTech199940(8)1-8[10][M]1996[11][M]1998[12]PCR_DGGE[J]201432(3)8-13[13][J]20126(11)4197-4202[14][J]201030(4)1-48[15]ChenGHWongMTOkabeSetalDynamicresponseofnitrifyingactivatedsludgebatchculturetoincreasedchlorideconcentration[J]WaterResearch2003[16]NaClKCl[J]200927(3)23-2530[17]PereboomJSizedistributionmodelformethanogenicgranulesfromfullscaleUASBandICreactors[J]WaterScienceandTechnology199430211-221[18]PCR-DGGE[J]201434(15)4288-4294[19]DGGE[J]201515(4)208-217