处理采矿废水湿地沉积物中厌氧氨氧化过程汪海波

39920189ENVIＲONMENTALSCIENCEVol．39No．9Sep．2018*230009、．．anaerobicammoniumoxidationANAMMOXanaerobicammoniumoxidationcoupledtoironreductionFeammox．FeammoxFeⅢ、、N2O．FeammoxFeammox1.69mg·kg·d－12.72mg·kg·d－1Feammox28%42%．ANAMMOX25%．ANAMMOX、Feammoxsulfate-reducinganaerobicammoniumoxidationS-ANAMMOXS-ANAMMOX．．ANAMMOXFeammoxX171A0250-3301201809-4215-07DOI10．13227/j．hjkx．2018011632018-01-172018-03-01413723471993～[e-mail]*[e-mail]ainWetlandSedimentsforTreatmentofMiningWastewaterWANGHai-boMADingYUEZheng-boTAOWeiCHENTian-huWANGJin*SchoolofＲesourcesandEnvironmentalEngineeringHefeiUniversityofTechnologyHefei230009ChinaAbstractNitrogenelementsplayanimportantroleinthebiogeochemicalcycleofartificialwetlands．Inthisstudyweinvestigatedtheanaerobictransformationofammonianitrogenanditsmainpathwaysinsedimentinanartificialwetland．ＲesultsshowedthattheanaerobicammoniaoxidationANAMMOXprocessandironreductionoccurredinwetlandsedimentcoupledwithanaerobicammoniaoxidationFeammox．FeammoxusedFeⅢtooxidizeammonianitrogentoproducenitrogenintermediateproductswerenitratenitriteandN2O．AdditionofferrihydritepromotedtheFeammoxprocessandammonianitrogenlosscausedbyFeammoxwasenhancedfrom1.69to2.72mg·kg·d－1．Whenferrihydritewasaddedalossof28%oftotalnitrogenTNinthewetlandoccurredassociatedwithFeammoxincreasingto42%．AnaerobicammoniaoxidationwassignificantlyinhibitedwithadditionofferrihydriteandTNlossinthesystemdecreasedbyabout25%．ＲesultsshowedthattheformationofgoethitebymineralizationofferrihydriteinhibitedtheANAMMOXprocesspromotingFeammoxtoleadtocompetingelectronicdonorsfortheS-ANAMMOXprocesscausinginhibitionoftheS-ANAMMOXprocess．ThisachievesthepurposeofreducingTNlossinthewetlandsystem．Inadditionthismayhavesomesignificanceforfurtherunderstandingtheinteractionbetweenironreductionandthenitrogencycleinthewetland．KeywordsammoniaanaerobicammoniaoxidationANAMMOXFeammoxferrihydriteminingwastewater、．．、1～4．、、．、、．．ANAMMOX、5～7．Feammox39、、8～13．Feammox．SO2－4、14．Feammox．11.1．6h．220mm．1∶399.99%32℃25d、、911．、、．1.210.0gpH≈6.52550.0mL30.0mLNH4Cl1719.49mg·L－190.0mg·kg－1．1316.0mg·L－1KH2PO4300.0mg·L－1MgCl2·6H2O74.0mg·L－1KCl840.0mg·L－1NaHCO3200.0mg·L－1NaCl180.0mg·L－1CaCl2·2H2O1.0mL·L－1．204.0mg·L－1ZnCl2240.0mg·L－1CoCl2·6H2O990.0mg·L－1MnCl2·4H2O149.0mg·L－1CuCl2·2H2O220.0mg·L－1Na2MoO4·2H2O190.0mg·L－1NiCl2·6H2O140.0mg·L－1H3BO4·2H2O210.0mg·L－1NaSeO4·10H2O．2552.0mL2.0mLNH4Cl2.0mLNH4Cl30.0%～3kPa2.0mLNH4Cl41.24mg2.0mLNH4Cl、41.24mg30.0%～3kPa．NH4Cl15minDO＜0.10mg·L－16.0mL99.99%30.0%～3kPa8．32℃15d1～2d100r·min－11.0min．1.3N2SP6890N2OVEＲTEX70H2SGMA33xxpH-Delt320．2mol·L－1KClNH+4、NO－2NO－32.0h5～10GMA33xx0.5mol·L－1HClFeⅡFeⅢFeⅡFeⅢ8SO2－41+1HClS600S2－AJ-210015SULTIMATE3000USA15WAYEE-WYS2200TOC．22.1N21．0.80±0.05mg·kg－10.05±0.003mg·kg·d－1．NH+463.17±5.34mg·kg－14.21±0.36mg·kg·d－170.15±612491.25%．NH+4+C2H225.35±3.32mg·kg－11.69±0.221mg·kg·d－127.05±2.01%．NH+4+FeⅢ41.07±5.17mg·kg－12.74±0.35mg·kg·d－145.44±0.98%．NH+4+FeⅢ+C2H238.18±1.23mg·kg－12.55±0.08mg·kg·d－142.29±2.11%．16C2H20.301～1.595mg·kg·d－11.561～1.786mg·kg·d－1C2H253.42%～169.70%．、、C2H2≥0.7kPaN2O1718ANAMMOXC2H2≥70Pa95%19FeammoxC2H27．1NH+4+FeⅢ+C2H2NH+4+FeⅢP＜0.05NH+4+C2H2NH+4P＜0.01ANAMMOX111319．NH+4+C2H2NH+4+FeⅢ+C2H2FeⅢFeammoxSO2－4S-ANAMMOX．307.59±65.21mg·kg－164.64±3.83mg·kg－1．15d284.11±25.48～295.1±38.73mg·kg－189.04±12.51～114.56±25.93mg·kg－11b．FeⅢSO2－4FeⅢSO2－420．S-ANAMMOX．NH+4+C2H2NH+4+FeⅢ+C2H2C2H2N2Feammox1．C2H20.19mg·kg·d－1Ding8．Yang1024hFeammox0.32mg·kg·d－1Li1224hFeammox0.24～0.36mg·kg·d－1FeammoxFeammox．6FeOH3+10H++2NH+46Fe2++18H2O+N2↑1115d/Fig．1Accumulativenitrogenproductionandsulfate/sulfidecontentinthereactionsystemafter15days2.2NH+4NH+4+C2H2FeⅢFeⅡ2FeⅢ13．NH+4+FeⅢNH+4+FeⅢ+C2H2FeⅡ3394.10±286.34mg·kg－12730.81±442.11mg·kg－1FeⅢ．NH+4NH+4+C2H2FeⅡ1139.97±123.93mg·kg－11043.50±54.69mg·kg－1FeⅡ2．NH+4+FeⅢNH+4197.36%NH+4+FeⅢ+C2H2NH+4+712439C2H2161.70%Feammox101221．NH+4+C2H2NH+4+FeⅢ+C2H2Feammox25.35±3.32mg·kg－138.18±1.23mg·kg－1Feammox91.69mg·kg·d－12.72mg·kg·d－1．Feammox9～12．FeⅢ2223．FeⅢFeammoxFeⅡ91113．215dFeⅡFig．2HCl-extractableFeⅡcontentinthereactionsystemafter15days1NH+4N2NH+4+FeⅢP＜0.01．FeⅢNO－2ANAMMOX24．FeⅡNO－2FeⅡFeⅢ25NO－2NO－3ANAMMOX22242627．NH+4+FeⅢNH+4+FeⅢ+C2H2NO－2ANAMMOX．NH+4+FeⅢFeammox0.19μg·kg·d－126．2.3N2ONO－2NO－3828．1μmol·L－129．．NO－3．NO－2．NH4Cl415dNO－34.43mg·kg－10.288μg·kg·d－1．Yang10FeammoxN2、NO－2NO－31～3．FeammoxNO－3NH4ClNO－3NO－3Feammox3．6FeOH3+10H++NH+46Fe2++16H2O+NO－228FeOH3+14H++NH+48Fe2++21H2O+NO－333NH+4、NH+4+C2H2、NH+4+FeⅢNH+4+FeⅢ+C2H2N2O16.22±1.07～31.46±1.67mg·kg－1N2O．N2O．C2H2N2O．NH+4+FeⅢ+C2H2N2ONH+4+C2H2P＜0.01N2O．26N2ON2O．FeammoxN2O12N2O2730．31N2ON2OpH、．31N2O81249N2O5.74mg·kg－1N2O．0.19μg·kg·d－1N2OFeammox4．8FeOH3+14H++2NH+48Fe2++23H2O+N2O↑4NH+4NH+4+FeⅢ415dNH+4NH+4+FeⅢFig．4NitrogenmassbalanceandlosspathsintheNH+4andNH+4+FeⅢsystemsafter15days315dN2OFig．3ProductionofN2Ointhereactionsystemafter15days2.4FeNO－2NO－332．NH+433NO－xANAMMOX9．NH+44NO－x．4FeⅢFeⅡ2．ANAMMOX20%．FeⅢ———Feammox8～13．15dNH+4NH+4+FeⅢ20%40%4．ANAMMOXNH+424.99mg·kg－112.83mg·kg－128%14%NH+4+FeⅢANAMMOX2.88mg·kg－13%．NH+4NH+4+FeⅢ76%51%912439A