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第24卷2(X)3年增刊6月中山大学学报(自然科学版)ATCASCIENTIARllMN人rFtIRALIUMUNIEVRSnTWISSANUY铭EMVoL24SulnPJ山1.2(jX31ExtracellularPolymersinBiogranulesTraetingBreweryanAdcetateWastewaters`AJI兀ao一shanl,FANGHed犯rtH护(1.及琴刃砚叮屺ntofEh彩ior~几如之肠舒配e月gn,及乙几aY卜sen乙hi浇sritr,公如刀脚切usl2.珠即冶砚以ofiC`11&娇溉ir咭,服nU~iytofH访堵彻鳍,杨刀g肠鳍,0275,hCianhCian)A加tract:iB(g)ranulestI’eiatgnb祀weyr~we二c~deabrlystorn罗rinesri~otattiritonadnid翻iuton,dueothetihghercontentofECP,山anhteacetate,de邵adignibo即an川es.E汤chgmofhtelatteribo邵an』eS,ihwch~p耐0而n阴dy几介之爪u理。动往忿,hadanvae“唱e0.的mgofECP,hweerashtefonneribo『anuleshad0.巧gm晚e~ofhetihghECPcon段einsinhethy枷liytc/fe~anttivehacietraonhets叫阮eandinhetsyn加内chaeteiraofhet而d-】ayer.Keywords:allaeorbic;ibo脚n』es;extarcellular卯l卿esr(EcP)CLCnu比nber:X172D叱u.lenta月e:AA币eleDI:0529一6579(2X()3)sl一0141一03Anaeoribcde脚diatonofoPllutanst15aconlPlexpor-eessillvolvingatleasthtere目orupsOfbaeetira.Hydorlytieandfemernaltivebacetiraevonerteo呷lexpollualnstintosimPleafttyacids,hwiehaerhetnfurtherevoenrted坊aceotgelllcbaeteiraintoaceatetandhydor罗n.iFnally,methano罗niebacteiraeonvertacetateand姚/C仇iontmeth~.nIhte卿tdecade,明aeorbieecthnologyhasbeenboradlyappliedothtet找分tnleinofmanyhihg-s腼hgt~tewat,,otave巧lal召eextent,duetohtesueeessfuldevelopmentofUASB(叩nowaaenbIDieslud罗blallket)pocress.nIaUASBeractor,bacetira典男ergateinotfo耐ngbio罗antlleshwihchavehihgbioactiviytandstlperbse已汕111妙[’〕.eReent,autie,vha。shown橱USABiboganrulesehxibitidffeerin而coturcuters,de-详ndignuepnhteehe而ealnatu比ofhetsubstarteinwastewaetradnthede脚diatonkineties[2〕.Althouhghte脚nulationmech画smofaaenobriebaeteirahasontbeenyetfollyudneot叮1,ithasbeen卿eulatedhtathetextmcellularoPl卿e。(ECp)eouldlikelypl即ani卿rtantolre〔,J.EeP,me回刃11。p耐-uestofbacteirahwiehaceumulaetonheteellsuafree〔4].hTeyportcetcensof〕mhetha玲hxeetmal~~in,adnP卿ideeellsiwhteenlgyande目bonhwenheterisa五减妇hsortage.tIhas】祀eneropertd场many~,he。htatECP15erlatdeothetflcoeuliatonofbaetieraleellsinae汕ic~~eretr~insysetm阎.I。。ereent,utdy〔2],u肠Bbiogranulestieart飞berweyr~tewaterweerfoundothavealaye初而-eorsturetuer初thdiffeontbacietral卯pulationineachvla·er.Hyd耐ytie/femernattivebacteirafomredadesneouetrlvaer,wheerassyntmPhiecoloniespolrifemtedinhte而d-layerandaceottorPhicmeht胡ogens~perd-o而~tinhetiinieroreoer.Onthehoterhand,bio即anulesteratignaceattee油ibitedasimPle,unifonn而eotucrt二per-tI15htusofinlhese幻泞oc。脱lation,ifbacetiraldis-itrbution.1MateiralS8ndmeth以sIiBogr阴ulest比atignberweyrwastewaterweerob-iatned血川1afoll一iszedUASB~for.iBo多an日esif习mhet~二`forhadPer”曲slybeenanalysedfor而-cosructr姗adnbacietralidsoutniton图.cAeatte一degard-ignhio邵anulesweer,onhetotllerhand,曲tained加ma2.8LUASlBaboaortyr~ot/7〕haignwast~r。on-tiaingn28X()mg/Lofaceaettvoerainen一monhtepired.dnIividualbiogl翔lulesweerhand一Piekedforhtissut街,50thatallibo歹叨ulesweer~hsnier司iwhtisesz斑nIggn1.9~2.1IlullandanleanofZ.Olll口1.hTeot回n山1让℃招ofbio罗anulespiekedforhtisstudyweer140forhtebewr-柳ibo罗anules,朋d100forhetaceaett一degardignib-卿叨记es仆ewottyl祀5ofibo歹anulesweouPtinseaP阳te157mLglassvials,eachofhwihccon面ned50mLof0.05%ofs诫unI洲iordesolutfon.仆esolutionineachvial~hten而xedusignaeTfl-oneoatedm哪笋eticl班atsoor/而n.hTeave咚罗s讹ofhetibo目旧ndeswas脚d-u吻获妇ueedvoeritmedueotsufareeattiritonandidsso-lution;~ofhetgarllules~~idsintegart司ashet而劝gnp似eeded.A丑eracert田nitmeintevral,hetsoliutonwasdecanedt.仆eave琢geis胎ofhetib咫邓11ules*收稿日期:2X()3一01一巧基金项目:香港RGC资助项目作者简介:贾晓珊l(%2年生),男,教授,博士生导师;Dm`l:画xs@suz.deu.cn142中山大学学报(自然科学版)第42卷nO4nUO日写s月写洲awsmeasuerdandthenun山erofbiog旧nulesawseount-ed.hTedceantedsolutionwasthenmeasuerdforistVSS(vofatilesuspendedsolids)adnECpCont。。st.Subse-qUently,thebio目旧nulesweouPtbaektohteglassvial,hwiehwasagalnifllediwth50一mLOffershsediumehio-irdesolution,andtheattirtio可dissolutionP似esswaser-拌ated.hTeporeesswaserepateduntilallhetbiog丑nulesbeeameottallydisintegartedatthaend.rflleVSSconlentinlhedeeanle(1solutionWas1llea-suedraceoldingtohteSatnd耐Mehtods.ECPwasex-t二tedinafoalnndehydesolutionat50℃[8〕followedby30而nutesofhihg一speedeenitrfugation(15仪旧G/而nat25X(K)G;m以lelJouan一KB22i).hTecarb〕11ydarteeon-tentofhteECP(ECcP)inhteextacretdsolutionwasmea-suerd饰thephenol/suiphuire一acidmehtod,andthepor-etineontent(CEpp)byhtefolinmethod.hTesumofECcPadnEC几wasthetotalECp.2Resulstsnd山Scu国sionAeetate一de脚di飞biogranules即详aerdtohvaelitileesristacneotthesufareeattirtio可dissolutioneausedbythestiirrngaction.iWhtin20ho明,allhtelX()bio邵扭1-ulesintheglassvialweertotallydisintegrated.BeeauseithapePnedunexepetedlyinsuchashort详ir记,hteerwasnosolutions歇npleeolleetedfor田lalysisduir飞hteinter-mediate.A刀alysisOftheifnalsolutionshowedhtateach呢ofaceatte一deg习di飞bio歹anules(asmeasu喇场VSS)eontainedanvaerageof0.伪毗OfECp.Onhteotherhand,b~叮biogranulesehxibiedtmuehstorngerersistancetowadrattirtion/dissolution.On-lywotoutofhtel40bio邵anulesweerdisintegratedinhteifsrt2311ousr.After47housr,twoaddiitonalbiog旧nuleswe比fuhlterdisientg旧ted.Eventually,allthebiog几nulesweertotallydisinte即旧tedafter110housr.Fig.1illus-atrteshtathtevaen嗯esizeOftheerlnianedbio脚nuleswas此dueedvoertlrne{lueotattnitox『dissolution.仆evae晓唱eadriuswaserdueedlienalryfl刀nlhteinitial1.X()unnto0.78mmafter8411ousr.Butallbio脚nulesweerdisi