400010AAOCarrousel2000TP。TP。。。INVESTIGATIONONTHEWAYOFANAEROBICPHOSPHORUSREMOVALINDOMESTICSEWAGEUNDERTRADITIONALTECHNOLOGICALCONDITIONSJiaoYuxinWangTaoWangDingchengHeYupingLiQierXuNaXiongWeiChongQingUniversityChongqing400010ChinaAbstractInthispaperonlybyaddingfillerstotheanaerobicpooloftraditionalAAOandCarrousel2000OxidationDitchprocesstheotheroperatingparameterswerethesameasthetraditionalprocessandtheanaerobicphosphorusremovalofthelowwaterintaketotalphosphorusconcentrationTPwasstudied.ThetestshowedthattheaverageTPconcentrationoftheanaerobicpooloutflowwaslowerthanthatofinflowundertheconditionofaddingthesuspendedmembranetotheanaerobicpoolandthelossofphosphoruswasfoundintheanaerobicpoolandthephenomenoncanbelongandrelativelystable.Thephosphorusbalanceanalysiswasfoundthattherewasacertainamountofphosphoruslossintheanaerobicsystemofcastingthehangandsuspendedfillersandthepresenceofphosphorusintheupperairwasdetected.Subsequentlyhigh-throughputsequencinganalysisofanaerobicsludgeandfillerswasusedasareferenceforthefutureresearchofdominantbacteria.KeywordsAnaerobicphosphorusremovalGaseousphosphideLowtotalphosphorusconcentrationDomesticsewage2018-01-270。PAOpoly-PhosphateAccumulatingOrganisms。PAODPBDenitrifyingPhosphorusRemovingBacteriaDPB。1。PAODPB1923Barrenscheen2。1927Rudakov3。1988Devai30%~40%-GC/MS25%~50%4-5。2000Jenkins6。2008Liu、7。。2005、98201836、、6。20097。、。AAOAAOAAOAAOTP。。。11.11.1.1AAOAAO--1Carrousel20001。0.3m3/d。COD350mg/LTN30mg/LNH+4-N20mg/LTP3.5mg/L。《》B。1AAOAAO1.44h0.018m32Carrousel20002.88h0.036m39.6h0.120m3。Carrousel20001.44h0.018m313.60h3.04h10.56h0.170m30.038m30.132m3。1.1.2AAOAAO+AAOAAO。+Carrousel2000。AAO0.3m3/d。AAO、AAO。AAO8%~10%。Turbed20mm。Turbed0.95kg/L。。≥95%。750m2/m3。1.209201836SS2.1.1。。AAOAAO。AAO0.3m3/d4000mg/L15d100%60%0.2mg/L0.5mg/L2~3mg/L22℃。。AAO8%~10%。24。。、、。22.1AAO30d、、。AAOTP、TN、NH+4-N、COD3、4。—■——○——★—。3AAO3、4AAOTP、TN、NH+4-N、COD《》BTN、NH+4-NCOD19201836—■——○——★—。4A。2.2AAO30d、、。AAOTP、TN、NH+4-N、COD5、6。5、6AAOTP、TN、NH+4-N、COD《》BTN、NH+4-NCODA。11TPTNNH+4-NCODAAO73.69%70.01%89.40%92.76%76.46%65.22%89.44%91.31%AAO70.69%71.39%88.92%93.75%71.74%64.80%90.11%92.95%1AAOAAO。TPTP。2.3TPAAOAAO、TPTP7、8。PAOsPAOs。PAOsTPTPTP。AAO+AAOTPTP7+TPTP8。AAOTPTP29201836—■——○——★—。5+AAO—■——○——★—。6+39201836—■——○——★—。7AAOTP—■——○——★—。8TP-0.668mg/L-0.639mg/LAAOTPTP0.783mg/L0.766mg/L。。8。DPB9。9。0.2mg/L。。TPTPTP4710-12。—■—AAO—○—。92.4。=TP-TP-TP。6L3LCOD、TN、NH+4-N、TP13mg/L26mg/L15%。2TPTPTP113.89mg/L492018362TPTPTPTPTP110.0711.17-8.70-6.29-14.9913.89218.8515.14-6.82-6.13-12.9516.66TPmg。216.66mg/L。3.3。AAOPAOs、TP。TPTPTP。TP3.3。。PAOsTP13-15。50mL0.5mol/LNaOH3000mL20%NaOH。pH2.010minNaOHpH7.0。。3。3TPTPmg/LTPmg/LNaOHTP201711246.783.751.51201711303.582.930.832017121615.1712.463.262017121812.7810.513.553。。。。2.5。DNA16SrRNA。16SrRNA338F/806R338FACTCCTACGGGAGGCAGCAG806RGGACTACHVGGGTWTCTAATPCR300~500bp。Illumina1210、1211。1—2—。101044Proteobacteria62.71%34.00%Actinobacteria6.87%16.75%Bacteroidetes7.56%15.46%Saccharibacteria7.38%10.33%Gemmatimonadetes1.56%6.72%Chloroflexi1.80%5.04%Parcubacteria2.37%3.41%Acidobacteria1.99%3.04%Firmicures2.08%1.55%Spirochaetae1.40%0.53%Cyanobacteria1.29%0.26%592018361—2—。114。Proteobacteria、Actinobacteria、Bacteroidetes、Chloroflexi、Acidobacteria、Firmicures、SpirochaetaeBacteroidetesAcidobacteria、Firmicures。BacteroidetesAcidobacteria。1155Tolumonas16.76%/Desulfomicrobium15.09%/Saccharibacteria6.10%9.71%Gemmatimonadaceae1.45%6.21%Micropruina0.78%4.55%Candidatus_Competibacter1.30%3.54%Zoogloea2.49%1.78%Haliangium1.00%3.68%Sulfurospirillum2.87%/Dechloromonas1.42%1.93%VadinHA171.01%2.18%Micrococcales1.28%1.77%Rhodobacter0.68%2.59%Methlocystaceae0.62%2.54%Desulfovibrio2.06%/Pleomorphomonas1.63%/Obscuribacterales1.23%0.23%。5Tolumonas、DesulfomicrobiumSulfurospirillumTPTP。MicropruinaObscuribacteralesObscuribacteralesMicropruina0.78%4.55%。PAOsPAOsTP·3.4TP=TP--TPTPTP。692018363TPTP。TP。、、。1BortoneGSaltarelliRAlonsoVetal.Biologicalanoxicphosphorusremoval-thedephanoxprocessJ.WaterScienceandTechnology1996341/2119-128.2BarrenscheenHBeckh-WidmanstetterH.UberbakteriellereduktionorganischgebundenerphosphorsaureJ.Biochem.Z1923140279-283.3RudakovK.DieReduktiondermineralischenPhosphateaufbiologischemWegeJ.Zentbl.Bakt.ParasitKdeAbt.II192770202-214.4DévaiIFelfldyLWittnerIetal.DetectionofphosphinenewaspectsofthephosphoruscycleinthehydrosphereJ.Nature19883336171343-345.5DevaiIDelauneR.EvidenceforphosphineproductionandemissionfromLouisianaandFloridamarshsoilsJ.OrganicGeochemistry1995233277-279.6DingLLiangHZhuYetal.Sourcesofmatrix-boundphosphineinadvancedwastewatertreatmentsystemJ.ChineseScienceBulletin200550121274-1276.7ZhouJLiuJChenYetal.PhosphorusRemovalbyBiologicalReductioninAnaerobicSequencingBatchBiofilmReactorforTreatmentofMustardTuberWastewaterJJ.ChinaWater&Wastewater200919004.8NgWOngSHuJ.Denitrifyingphosphorusremovalbyanaerobic/anoxicsequencingbatchreactorJ.Waterscienceandtechnology2001433139-146.9ZengRJLemaireRYuanZetal.Simultaneousnitrificationdenitrificationandphosphorusremovalinalab-scalesequencingbatchreactorJ.Biotechnologyandbioengineering2003842170-178.10