纯氧曝气活性污泥培养过程中絮体结构变化胡小兵

36320163ActaScientiaeCircumstantiaeVol．36No．3Mar．2016No．KJ2013A059SupportedbytheScienceＲesearchProjectofCollegesandUniversitiesinAnhuiProvinceNo．KJ2013A0591966—[e-mail]*[e-mail]1966—[e-mail]*[e-mail]10．13671/j．hjkxxb．2015．0498．2016．J．363907-913HuXBYeXＲaoQetal．2016．ＲesearchonthechangeofflocstructureintheprocessofactivatedsludgeculturewithpureoxygenaerationJ．ActaScientiaeCircumstantiae363907-91312*111111．2430322．2430002015-05-042015-05-272015-05-27SBＲ、Deq、Ｒd、PoFtlPearson．DeqDeqＲdＲd0．400～0．520、PoPo、Po、．DeqPoＲdDeq、PoFtlPo、Ｒd．DeqＲd、PoＲd．0253-2468201603-907-07X703AＲesearchonthechangeofflocstructureintheprocessofactivatedsludgeculturewithpureoxygenaerationHUXiaobing1YEXing2*ＲAOQiang1ZHUＲongfang1ZHAOXin1ZHOUYuankai1ZHONGMeiying11．CollegeofArchitecturalEngineeringAnhuiUniversityofTechnologyMa'anshan2430322．CollegeofEnergyandEnvironmentAnhuiUniversityofTechnologyMa'anshan243000Ｒeceived4May2015receivedinrevisedform27May2015accepted27May2015AbstractActivatedsludgewereculturedbytwowaysofdirectcultureanddomesticatedincubationinsequencingbatchreactorSBＲbypureoxygenaerationrespectively．MicroscopicanalysistechnologywasusedtoanalyzethechangeofAggregateEquivalentdiameterDeqＲegularitydegreeＲdPorosityPoandTotalfilamentslengthpertotalsuspendedsolidsFtlofthesludgefloc．ThecorrelationsamongsludgeflocstructurecharacteristicparametersweredrawnbythemethodofPearsoncorrelationanalysis．TheresultshowsthatthesizeofsludgeflocDeqincreasesduringtheprocessofdirectculturecorrespondinglyflocDeqindomesticatedincubationdecreasesduringtheearlyphasebutconstantlyincreasesfromthemiddletothelatephase．largersizeofsludgeflocparticlesisobtainedindomesticatedincubationmethod．SludgeflocＲdchangeslittleandfluctuatesbetween0．400and0．520indomesticatedincubationwhichissimilartoflocＲdindirectcultureprocess．SludgeflocPoincreasesalongwithsizeofflocparticlesatearlyandmiddlestagesofdirectcultureanddecreasesatearlyandlatestagesofdomesticatedincubation．ThevaluesofflocPoindirectculturemethodarelow．Agreatnumberoffilamentousbacteriaareobservedemergingatmiddleandlatestagesofdirectculturebutthendecreasedramatically．Littlefilamentousbacteriaappearinthewholeprocessofdomesticatedincubation．DuringtheprocessofdirectculturepositiveandnegativecorrelationsareobservedbetweenflocDeqandflocPoandflocＲdandflocDeqPorespectivelyattheverybeginning．FilamentousbacteriaplaysanimportantroleandsludgeflocFtlaresignificantlyandnegativelycorrelatedwithflocPoandＲdatthemiddlestageintheprocessofdirectculture．AtthebeginningstageofdomesticatedincubationsludgeflocDeqaresignificantlyandpositivelycorrelatedwithsludgeflocＲdandPoflocＲdplayssignificanteffectatthelateperiodofdomesticatedincubationandaresignificantlyrelatedwithallotherparameters．Keywordspureoxygenaerationactivatedsludgedirectcultureactivateddomesticatedincudationflocstructure361IntroductionHanetal．2012、、Costaetal．2013Wilénetal．2003Nasser2014WalaszekandAy2006．、Lietal．2008．Mesquitaetal．2011Turtinetal．2006．2013．Chenetal．2003ZupancˇicˇandＲo2008Ｒodríguezetal．2012．、、．、．SBＲ．、、．2Materialsandmethods2．17．20cm92．00cm3．20L0．1L·min－16．00—8．00mg·L－1．1、NH4ClKH2PO4100∶5∶1COD、NH3-N、TP1000．00±100．00mg·L－1、50．00±10．00mg·L－1、5．00±2．00mg·L－1．720min、5min、600min、115min．MLSS4744．00mg·L－11．．2．2、、Deq、Ｒd、PoFtl．DeqFtlCostaetal．2013．1．Ｒd=2π·槡AP1Amm2Pmm．．Po=∑Ni=1ApiTA2Apimm2TAmm2．Costaetal．20132013．0．05mLBX53MshotDC30MShotDigitalImagingSystem1024×768JPEG．80320．Image-proPlus、、HSI8093、、、Excel．、1990．3Ｒesultsanddiscussion3．1MLSS．Luna-Pabelloetal．1990Abrahametal．1997Leeetal．2004Madoni2005MLSS、．1MLSSFig．1TheMLSSchangeduringtheprocessofactivatedsludgecultureMLSS1．MLSS22d3941．00mg·L－1．1～7d．8d22d938ind．·L－1．23d、35d220ind．·L－1．35dCOD95．10%．1～7d、8～22d、23～35d、、．10dMLSS10dMLSS2637．00mg·L－11290．00mg·L－1．25ind．·L－1238ind．·L－1．11d22d1575ind．·L－1．23d31d220ind．·L－1COD97．31%．1～10d、11～22d、22～31d、、．、．4d．3．22DeqFig．2ThechangeofflocDeqduringtheactivatedsludgeculture3．2．1、23、4．Deq1．00μm250．90μm．Deq．、Deq90936Deq．3ＲdFig．3ThechangeofflocＲdduringtheactivatedsludgecultureDeq．Deq196．00μm111．40μmDeq31d281．50μm．、．、Jarvisetal．2005．Deq．Deq．EPSDeq．．3．2．2Ｒd3Ｒd0．8730．339．、、4a～c．Ｒd0．400～0．520．4d～f．4a～c5、21、35dd～f5、21、31dFig．4ThechangeofflocshapesduringtheprocessofactivatedsludgecultureFlocsintheprocessofdirectculturea～cfrom5、21、31drespectivelyflocsintheprocessofdomesticationd～ffrom5、21、31drespectivelyＲd．0193VahediGorczyca2011．DeqDeq130．00μm、Deq＞130．00μmＲd0．520Ｒd．Ｒd0．400．3．2．3Po5．Po0．008～0．049、Po．Po0．030～0．120、Po．5PoFig．5ThechangeofflocPoduringtheprocessofactivatedsludgecultureDeqPo2、5Deq104．00μm．Sposito1997-．Deq104．00μm-YangandLi2009Shengetal．2010Wangetal．2014．PoPoPo．3．2．4Ftl6．16d0～0．40μm·mg－1．Martinsetal．20044b31dFtl30．50μm·mg－1SVI41．90mL·g－1．、、35d3．00μm·mg－1．-Martinsetal．20042000Deq．6FtlFig．6ThechangeofflocFtlduringtheprocessofactivatedsludgeculture3．3、．、Pearson7．DeqPor=0．996p＜0．05Deq1．00μm．ＲdDeq、ＲdPor－0．945p＜0．05－0．97p＜0．05．－119367a．b．Fig．7Thecorrelationcoefficientmatrixgraphofflocstructureparameter