纯种氨氧化细菌Comamonas省略L3的固定化及短程硝化性能研究李正魁

ComamonasaquaticLNL3,,,,,,(,,　210093):,Poly(HEA)-Poly(HEMA)AOB-ComamonasaquaticLNL3,AOB、pH、DO、FA.,AOB-ComamonasaquaticLNL3,AOB-ComamonasaquaticLNL3、pH、DO、FA30℃、8.5、4.03mgL9mgL,93.52%、94.73%;79.74%、94.67%;91.17%、94.66%90%、94.4%,FA.:LNL3;;;;:X703.1　:A　:0250-3301(2009)10-2952-06:2008-11-21;:2009-01-04:(973)(2008CB418003);(863)(2008ZX07101-012-04-03,2008ZX07101-004);(2006BAJ08B01-02):(1959～),,,,,E-mail:[e-mail]-oxidizingBacteriaComamonasaquaticLNL3andItsPartialNitrificationCharacterizationLIZheng-kui,SHILu-na,YANGZhu-you,ZHANGXiao-jiao,WANGYue-ming,CHENQi-chun,WUKai(StateKeyLaboratoryofPollutantControlandResourcesReuse,SchoolofEnvironment,NanjingUniversity,Nanjing210093,China)Abstract:Anewkindofammonia-oxidizingbacteria(AOB)-ComamonasaquaticLNL3wasscreenedoutandimmobilizedbyPoly(HEA)-Poly(HEMA)copolymercarrierusingirradiationtechniques.Fourkindsofimpactfactorsonshort-cutnitrification,includingtemperature,pH,DOandfreeammonia(FA)concentrationhadbeeninvestigated.TheresultshowedthatAOB-ComamonasaquaticLNL3hadshort-cutnitrificationcapabilityandtheoptimaltemperature,pH,DOandFAconcentrationwere30℃,8.5,4.03mgLand9mgLrespectively.Correspondingtoaboveresults,ammonianitrogenremovalrateandshort-cutnitrificationefficiencywere93.52%,94.73%;79.74%,94.67%;91.17%,94.66%and90%,94.4%respectively.Keywords:ComamonasaquaticLNL3;short-cutnitrification;copolymercarrier;ammonianitrogenremovalrate;short-cutnitrificationefficiency　　,,,SHARON、ANAMMOX、OLANDCANON[1～6],,,,、,(ammonia-oxidizingbacteria,AOB).,AOB.AOB,、,,AOB(Nitrosomonas)、(Nitrosospira)、(Nitrosolobus)、(Nitrosovibrio)(Nitrosococcus)[7,8].AOB,.(、DO、pH、)[9～12],,AOB.AOBTanaka[13],.、,16SrRNAGenBankBLAST,AOB-LNL3(ComamonasaquaticLNL3),.,AOB-ComamonasaquaticLNL3,,.,poly(HEA)-Poly(HEMA)3010200910　　　　　　ENVIRONMENTALSCIENCEVol.30,No.10Oct.,2009DOI:10.13227/j.hjkx.2009.10.020,[14].,AOB-ComamonasaquaticLNL3,,、pH、、DOComamonasaquaticLNL3,.1　1.1　[2-hydroxyethylacrylate,HEA]、β-[2-hydrosyethylmethylacrylate,HEMA](Fluka,)..1.2　AOBAOB-(ComamonasaquaticaLNL3)2008124“”,CGMCCNo.2361,200810020649.4.、、.AOB-(ComamonasaquaticaLNL3):(NH4)2SO42.0g、K2HPO40.5g、MgSO40.5g、FeSO4·7H2O0.4g、NaCl0.5g、Na2CO31.0g,1000mL,pH7.2.1.3　LRH-150()、THZ-82A()、T52N()、5000ATOC()、PHS-3CpH()、JPSJ-605()、60Co-γ()、HITACHIS-3400N().1.4　2-Hydroxyethylacrylate(HEA)、β-2-Hydroxyethylmethylacrylate(HEMA),,60Co-γPoly(HEA)-Poly(HEMA)[15].1.5　AOB-ComamonasaquaticaLNL3,28℃,3.8×108MPNL;AOB,28℃24h3,,AOB500mL,,.1.6　,(NH4)2SO4,NaHCO310ggNH+4-N,、,pH8.0(pH).1.7　NH+4-N:,NO-2-N:N-(1-)-,NO-3-N:,:[8];pHPHS-3CpH,DOJPSJ-605.1.8　1.;2.;3.;4.;5.;6.;7.;8.;9.;10.1　Fig.1　Schematicdiagramofexperimentalreactor1.1.7L(8cm,50cm)(30℃±1℃),,,,10%,,NH+4-N、NO-2-NNO-3-N.(1)(2).=NH+4-N-NH+4-NNH+4-N×100%(1)295310:ComamonasaquaticLNL3=NO-2-N(NO-2-N+NO-3-N)×100%(2)2　2.1　AOB-ComamonasaquaticaLNL360Co-γHEAHEMA,Poly(HEA)-Poly(HEMA),HITACHIS-3400N.,,5～30μm(2),1～6,[16,17];,HEAHEMA—OH,,[18],AOB-ComamonasaquaticaLNL3;,SEM,Poly(HEA)-Poly(HEMA),1,AOB-ComamonasaquaticaLNL3,、,AOB,AOB、,AOB(3).MPN,AOB-ComamonasaquaticaLNL31.6×106MPNL.2　Fig.2　Structureofcopolymercarrier　2.2　AOB-ComamonasaquaticaLNL3AOB,.3　Fig.3　StructureofcopolymercarrierandAOBafterexperiment　,,,,,;,,;,,,.,,.Knowles[19],AOB,AOB,AOB.,AOB-ComamonasaquaticaLNL3,15℃30℃,,93.52%94.73%,35℃,AOB-ComamonasaquaticaLNL3,91.76%91.07%.AOB-ComamonasaquaticaLNL330℃(4).2.3　pHAOB-ComamonasaquaticaLNL3AOB,pH.pH:,AOB;,AOB;,AOB;,[20],Gernacy[21]AOBpH,AOBpH7.0～8.5.,pHAOB-ComamonasaquaticaLNL3,pH6.0,AOB-ComamonasaquaticaLNL32954　　　　　　304　AOB-ComamonasaquaticaLNL3Fig.4　Effectoftemperatureonshort-cutnitrificationofimmobilizedAOB-ComamonasaquaticaLNL3　5　pHAOB-ComamonasaquaticaLNL3Fig.5　EffectofpHonshort-cutnitrificationofimmobilizedAOB-ComamonasaquaticaLNL3pH,pH7.0,39.65%89.24%,pH8.5,,79.74%94.67%,pH9.0,,58.31%91.98%.pH,pH8.5,pH8.5,pH8.5,,pH8.09.0pH8.599.26%、81.3%97.15%、81.30%.,AOB-ComamonasaquaticaLNL3pH7.0～9.0,AOB,,pH8.5,　　　　(5).2.4　AOB-ComamonasaquaticaLNL3AOB-ComamonasaquaticaLNL3,.AOB,,.,.Keisuke[22],(DO0.5mgL)AOB,Laanbroek[23]AOBAOB.,AOB-ComamonasaquaticaLNL3.DO4.03mgL,,DO=2.38mgL,AOB-ComamonasaquaticaLNL3,71.63%94.05%,DO3.05mgL,77.57%94.5%,DO=4.03mgL,,91.17%94.66%,,.,AOB-ComamonasaquaticaLNL34.03mgL(6).,AOB,AOB-ComamonasaquaticaLNL3,,AOB-ComamonasaquaticaLNL3,,DO,AOB-Comamonas　　　　6　AOB-ComamonasaquaticaLNL3Fig.6　EffectofDOonshort-cutnitrificationofimmobilizedAOB-ComamonasaquaticaLNL3295510:ComamonasaquaticLNL3aquaticaLNL3;,,,AOB,,,.,AOB-ComamonasaquaticaLNL3DO4.03mgL,AOB-ComamonasaquaticaLNL3.2.5　(NH+4-N)AOB-ComamonasaquaticaLNL3pHNH3(FA)NH+4-N.NH+4-NFA(3).NH3+H2ONH+4+OH-(3)　　FApHNH+4-N,AnthonisenJoanna[24].FA=1714×NH+4-N×10pHexp6334273+T+10pH(4)7　(FA)AOB-ComamonasaquaticaLNL3Fig.7　EffectofFAonshort-cutnitrificationofimmobilizedAOB-ComamonasaquaticaLNL3　　(FA)AOB-ComamonasaquaticaLNL3:(30±1)℃,85.21～518.9mgL,pH,pH7.5.(4)(FA)2.66～16.2mgL.7,(FA),FA=9mgL,90%,FA.,,AOB-ComamonasaquaticaLNL3NH3NH+4,NH3,,[6].5,(FA)9mgLAOB-ComamonasaquaticaLNL3.,(FA),2.66～16.2mgL,85%～95%,.,AOB-ComamonasaquaticaLNL3,(FA),.3　(1)Poly(HEA)-