磁修饰酵母对直接大红染料的生物吸附

生物工程学报ChinJBiotech2009,October25;25(10):1477-14[e-mail]©2009InstituteofMicrobiology,CAS&CSM,AllrightsreservedReceived:March30,2009;Accepted:August18,2009Correspondingauthor:QiWu.Tel:[phone];Fax:[phone];E-mail:[e-mail]环境生物技术吴琦,单志,沈茂,李双江,陈惠,625014:本研究采用水相合成的纳米级铁磁流体对灭活酿酒酵母细胞进行磁修饰,获得了具有良好磁响应的酵母。傅立叶变换红外光谱分析表明,该修饰酵母在Fe-O特征峰581cm−1处的吸收明显加强。透射电镜观察表明,纳米磁性颗粒单个或成团聚集在酵母细胞表面。在本实验条件下,160μL磁修饰酵母对1mL浓度为0.4mg/mL直接大红染料吸附率达100%;8min达到吸附平衡;在70%乙醇中,染料脱吸附率为99.18%。由于磁修饰酵母吸附力强,吸附速度快,易于磁分离,是一种有前景的水溶性染料生物吸附剂。:酿酒酵母,磁修饰,直接大红,生物吸附BiosorptionofdirectscarletdyeonmagneticallymodifiedSaccharomycescerevisiaecellsQiWu,ZhiShan,MaoShen,ShuangjiangLi,andHuiChenCollegeofLifeScience,SichuanAgriculturalUniversity,Ya’an625014,ChinaAbstract:Wepreparedthenano-sizedmagneticfluidinaqueousphasesolutiontomodifythekilledyeastcells(Saccharomycescerevisiae).Subsequently,theyeastcellswithgoodmagneticresponsewasobtained.TheinfraredspectroscopyanalysisshowedthatthemodifiedyeastcellsincreasedobviouslytheabsorptionattheFe-Ocharacteristicpeakof581cm−1.Moreover,thetransmissionelectronmicroscopypictureindicatedthemagneticnanoparticlespresentedindividuallyoraggregatelyontheyeastcellsurface.Undertheexperimentalconditions,themagneticallylabeledyeastwith160μLshowedtheadsorptionpercentageupto100%in1mLdirectscarletdyesolutionwiththeconcentrationof0.4mg/mL,andarrivedattheadsorptionequilibriumwithin8min.Besides,theadsorbeddyescanbeelutedin70%alcoholwithadesorptionpercentageof99.18%.Becauseofthestrongadsorptioncapacity,thehighadsorptionspeedandeasyofmagneticseparation,themagneticallymodifiedyeastcellsarethepromisingbiosorbentcandidatesforremovalofwater-solubledyes.Keywords:Saccharomycescerevisiae,magneticmodification,directscarlet,biosorption7010,2%~50%[1][2],,(10~50mg/L)[3],4BE,,1478ISSN1000-3061CN11-1998/QChinJBiotechOctober25,2009Vol.25No.10Journals.im.ac.cn4BE,[4][5]/-[6]4BE,,,80,,[7-8]Šafařik[9]Aksu[10],,,,[11-12],:,,,,,,,,[13],,4BE,1材料与方法1.1材料1.1.1(Saccharomycescerevisiae),1.1.2:4BE(28),(congored,C32H22N6Na2O6S2),,1g/L,pH6YPD:(0.5g1g2g,100mL,pH);(,1.8g)1.1.3UNICOTMWFZUV-2102C();Hitachi-6010();FTIR-8400S()1.2方法1.2.1Shan[14]:100mLNaOH(4mol/L),63oC30min36mLFeCl3·6H2O(0.5mol/L)20mLFeSO4·7H2O(0.5mol/L),300r/min,1h,,20mg/mL1.2.2(Saccharomycescerevisiae)YPD,,30oC200r/min5%,30oC200r/min24~36h8000r/min5min,5,OD600=60,2min,4oC300μL100200300400500600μL,15min20min,(8000r/min,5min)OD600(P0.05),80oC24h,,,(TEM)1.2.3,,-,563nm504nm2mmol/L(NaOH)1g/L,2mmol/L(NaOH)(01020406080100mg/L),563nm(504nm),C,:1479Journals.im.ac.cnA,C-A=(A0−A1)/A0×100%,A0,A1;=W1/W0×100%,W1,W01):400µL0.2mol/L10µL,20406080100150200250μL,,1mL,,30min,2):400µL0.2mol/L10µL,,1mL,,,13582030min,3):2),30min,,20%40%50%60%70%80%90%1mL(0.2mol/LNaOH20μL)20min,2结果与分析2.1酵母细胞的磁修饰1,1,OD6000,(P0.05),,1:12,,;,2.2磁修饰酵母的红外光谱33,33424cm−1,581cm−1,,Fe-O[15];,,,Fe3O4,(85%),,图1酵母细胞的磁修饰Fig.1Yeastcellsmodifiedwithmagneticfluid.Vm:volumeofmagneticfluid;Vy:volumeofyeastcells.图2磁修饰酵母对外磁场的磁响应Fig.2Responseofmagneticallymodifiedyeastcellstoexternalmagneticfield.(A)Suspensionofyeastcells.(B)A+externalmagneticfield.(C)Suspensionofmagneticallymodifiedyeastcells.(D)C+externalmagneticfield.1480ISSN1000-3061CN11-1998/QChinJBiotechOctober25,2009Vol.25No.10Journals.im.ac.cn图3磁修饰酵母的红外光谱Fig.3Infraredspectroscopyofmagneticallymodifiedyeastcells.4,,,,,,,Azevedo[16],,图4磁修饰酵母的透射电镜图Fig.4TEMofmagneticallymodifiedyeastcell.2.4磁修饰酵母对直接大红的吸附2.4.15,0.4mg/mL,98%:200μL100μL160μL,98.6%99.89%100%160μL80μL,59.87%95.45%,,,图5吸附材料浓度对吸附率的影响Fig.5Effectsofadsorbentconcentrationonadsorption.2.4.22.4.1,3,6,,30min,81.47%:1min,,99.46%,;1min93.55%,8min,,99.64%,,,2.4.3,,7,60%80%70%,,55.17%96.79%99.18%,,,,,,100%:1481Journals.im.ac.cn图6吸附材料的吸附时间曲线Fig.6Adsorptiontimecurveofadsorbents.图7乙醇浓度对脱吸附作用的影响Fig.7Effectsofalcoholconcentrationonadsorbents’desorption.3讨论,,,,,,,,,[17],(γ-Fe2O3)(Fe3O4)[18-19],(Immunomagnetic)[20],[21],[22]Šafařik[9,23],Fe3O4(Saccharomycescereviside)(Kluyveromycesfragilis),Fe3O4,,,,Safarik[23]Yavuz[24]Safarikova[25](Chlorellavulgaris),,,,,,Fe-O,,Šafařik[16],,,,(Anilineblue)220mg/g,262mg/g,,,8min,,,,,,,,1482ISSN1000-3061CN11-1998/QChinJBiotechOctober25,2009Vol.25No.10Journals.im.ac.cn,,REFERENCES[1]ZollingerH.Azodyesandpigments.ColourChemistry:Synthesis,PropertiesandApplicationsofOrganicDyesandPigments.NewYork:Wiley-VCH,1987:92−100.[2]HanCX,CaoM,ZhangBG.Progressofflocculationappliedtodyeingwastewatertreatment.IndWaterTreat,2006,26(9):73−76.,,..,2006,26(9):73−76.[3]BanatIM,NigamP,SinghD,etal.Microbialdecolorizationoftextile-dye-containingeffluents:areview.BioresourceTechnol,1996,58(3),217−227.[4]MaZC,JiangLH,DongLL.Studyondecolorizationofdirectred-containingwastewaterbyfreshMnO2adsorption.EnvironProtChemInd,2002,22(1):38−41.,,..,2002,22(1):38−41.[5]WangCT,WangQ,HeQH,etal.Astudyonthetreatmentofdirectscarletdyewastewaterbychlorinedioxidecatalyticoxidationonactivecarboncarrier.DyesColor,2006,43(3):49−52,55.,,,..,2006,43(3):49−52,55.[6]BaiB,ChenZH,WangLP.Treatmentofdirectscarlet4BEdyewa