从污水中分离出菌株DB1去除地下水硝酸盐实验刘咏

　第31卷第10期　2008年10月合肥工业大学学报(自然科学版)JOURNALOFHEFEIUNIVERSITYOFTECHNOLOGYVol.31No.10　Oct.2008　　　:2007-11-12;:2008-02-25:(40672154);(NCET-06-0541)(07010302165):　(1968-),,,;(1968-),,,,,.DB1刘　咏1,　钱家忠2,　李云霞2,　李如忠2(1.,　230009;2.,　230009)　:NO3--N、NO2--N,DB1;,NO3--N96.16%,NO3--N,100%;NO3--NNO2--N,NO2--N。:;;;:X523;P641　　　:A　　　:1003-5060(2008)10-1565-04ExperimentalstudyonnitrateremovalfromgroundwaterbytheanaerobicstrainDB1isolatedfromthesewageLIUYong1,　QIANJia-zhong2,　LIYun-xia2,　LIRu-zhong2(1.SchoolofBiotechnologyandFoodEngineering,HefeiUniversityofTechnology,Hefei230009,China;2.SchoolofResourcesandEnvironmentalEngineering,HefeiUniversityofTechnology,Hefei230009,China)Abstract:ThedenitrificationactivityofthestrainwasinvestigatedthroughdeterminingNO3--N,NO2--Nandconcentrationofthestraininmedium.ResultsshowthattheremovalefficiencyofNO3--Nreached96.16%inwater,andcompletedenitrificationwasachievedinsimulatedaquiferwiththeremovalefficiencyof100%;theremovalofNO3--NandaccumulationofNO2--Nmainlyhappenedintheexponentialphase.TheremovalofNO2--Nmainlyhappenedinthestableandincli-ningperiods.Keywords:denitrobacteria;anaerobicstrain;nitrateremoval;aquifer　　2060。、,,(),,,[1-4]。。NO3--N,()[5],NO2-[6]。[7,8],,,,,[7],,[8],。、。、、,NO2-、NO、N2ON2[9],、[7]。,,,、、、、、[10]。,。,,[11],[12],。、,,,[13,14],,,。,,,。1　(1)。2mL100mL(10g,KNO31g,1000mL),32℃24h,5;1mL,;24h。(5g,KNO32g,KH2PO41g,Na2HPO41g,MgSO4·7H2O0.2g,20g,1000mL),32℃24h,3。,,2mL100mL(5g,KNO32g,KH2PO41g,Na2HPO41g,MgSO4·7H2O0.2g,1000mL),7d,。(2)。1000mL600mL(5g,KNO32g,KH2PO41g,Na2HPO41g,MgSO4·7H2O0.2g),2%,32℃,,、,3。(3)。10cm,,:1248.0g/cm3,0.33,。,,。55cm,2cm,,。,[15],,,,,,。30℃,2%。、。,。(4)。[16];N-(1-)-[17];[17];[18]。2　2.1　18,3:①、,;②、,、;③、、。4,1、2、3、4。,4,3,4。1、2、37d,1566　　　合肥工业大学学报(自然科学版)第31卷　75.6%、43.56%62.04%,1,DB1。2.2　DB1(1)DB1。DB1NO3--N、NO2--N,1。1　1,DB1、、。NO3--N,DB110h,34h,,NO3--N,NO2--N,DB1NO3--NNO2--N,36h,NO2--N,NO2--N,;60h,NO2--N,NO2--N,NO3--N。140hNO3--N96.16%。(2)DB1。,NO3--N、NO2--N,DB1,2。2　NO3--N72h146.3mg/L2.8mg/L,;NO2--N24h,102.1mg/L,24h,,NO3--NNO2--N100%。2NO3--N,NO3--N,,;NO2--N,;2,,140h120h,,;,,NO3-※NO2-※NO(g)※N2O(g)※N2(g),。3　　　(1)DB1,、、、,。(2)DB1,DB196.16%;,120h100%。(3)DB1,。[　　　][1]　,.[J].,2004,(4):22-26.[2]　,　,,.[J].,2004,19(2):183-191.[3]　,,.15N18O[J].,2001,20(4):95-108.[4]　,,,.1567　第10期刘　咏,等:从污水中分离出菌株DB1去除地下水硝酸盐实验[J].,2006,28(1):102-107.[5]　HaugenKS,SemmensMJ,NovakPJ.Anovelinsitutechnologyforthetreatmentofnitratecontaminatedgroundwater[J].WaterResearch,2002,36:3497-3506.[6]　KesseruP,KissI,BihariZ,etal.Biologicaldenitrificationinacontinuous-flowpilotbioreactorcontainingimmobilizedPseudomonasbutanovoracells[J].BioresourceTechnolo-gy,2003,87:75-80.[7]　TangCY,AzumaK,IwamiY,etal.Nitratebehaviorinthegroundwaterofaheadwaterwetland,Chiba,Japan[J].HydrologicalProcesses,2004,18:3159-3168.[8]　BohlkeJK,WantyR,TuttleM,etal.Denitrificationintherechargeareaanddischargeareaofatransientagricul-turalnitrateplumeinaglacialoutwashsandaquifer,Min-nesota[J].WaterResourcesResearch,2002,38(7):1105-1129.[9]　OvezB,OzgenS,YukselM.BiologicaldenitrificationindrinkingwaterusingGlycyrrhizaglabraandArundadonaxasthecarbonsource[J].ProcessBiochemistry,2006,41:1539-1544.[10]　,.[M].:,2005:170-172.[11]　SmithRL,BuckwaterSP,PepertDA.Small-scale,hy-drogen-oxidizing-denitrifyingbioreactorfortreatmentofnitrate-contaminateddrinkingwater[J].WaterResearch,2005,39:2014-2023.[12]　RezaniaaB,OleszkiewiczaJA,CicekbN.Hydrogen-de-pendentdenitrificationofwaterinananaerobicsubmergedmembranebioreactorcoupledwithanovelhydrogendeliv-erysystem[J].WaterResearch,2007,41:1074-1080.[13]　KimYJ,YoshizawaM,TakenakaS,etal.IsolationandcultureconditionsofaKlebsiellapneumoniaestrainthatcanutilizeammoniumandnitrateionssimultaneouslywithcontrolledironandmolybdateionconcentrations[J].BiosciBiotechnolBiochem,2002,66(5):996-1001.[14]　SilvaSR,GingPB,LeeRW,etal.Forensicapplica-tionsofnitrogenandoxygenisotopesintracingnitratesourcesinurbanenvironments[J].EnvironForensics,2002,3(2):125-130.[15]　　,　,　.[J].,2004,24(3):504-507.[16]　　,,.[M].:,2002:55-78.[17]　,,　,.[M].4.:,2003:211-274.[18]　　.[M].:,2005:70-72.(　　)(1564)(3),,。(4),,,。[　　　][1]　AslanS,CakiciH.Biologicaldenitrificationofdrinkingwa-terinaslowsandfilter[J].JournalofHazardousMaterials,2007,148:253-258.[2]　.[M].:,1988.[3]　DellaRoccaC,BelgiornoV,MeriS.Heterotrophic/auto-trophicdenitrification(HAD)ofdrinkingwater:prospec-tiveuseforpermeablereactivebarrier[J].Desalination,2007,210:194-204.[4]　SchipperLA,Vojvodic-VukovicM.Nitrateremovalfromgroundwateranddenitrificationratesinaporoustreatmentwallamendedwithsawdust[J].EcologicalEngineering,2000,14:269-278.[5]　,　,,./[J].,2006,22(16):16-19.[6]　SchipperLA,Vojvodic-VukovicM.Fiveyearsofnitrateremoval,denitrificicationandcarbondynamicsinadenitrifi-cationwall[J].WatRes,2001,35(14):3473-3477.[7]　,,.[J].,2004,23(3):512-515.[8]　SuChunming,PulsRW.Removalofaddednitrateincot-tonburrcompost,mulchcompost,andpeat:mechanismsandpotentialuseforgroundwaternitrateremediation[J].Chemosphere,2007,66:91-98.[9]　.[M].4.:,2002:258-274.[10]　SchipperLA,Vojvodic-VukovicM.Hydrauliccon-straintsontheperformanceofa