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25120103 JournalofAnhuiUniversityofTechnologyandScienceVol.25.No.1Mar.,2009:2009-09-24 :(08040102002):(1983-),,,.:(1956-),,,,.:1672-2477(2010)01-0011-04-郑西强,蔡昌凤＊,李 娜(, 241000):-,3,,-;、2.67(∶=12∶4.5);(1000r/min、10min).,,. :;;;:X172;Q-33 :A[1-3],Mudd1924,,[4].[5],.,,.1 1.1 :/ZetaZetasizerNanoZS90(BROOKHAVEN);(IR-21);TU-1901();KYC-1102C();();TDL-40B().:CCL4,C8H18O,C6H4(CH3),.(≥18MΨ·cm),(10)、(4).1.2 -600nm,,(A).(C)(A),A=kC,k.[6-7],,.,r=(C0-C1)/C0×100%=(A0-A1)/A0×100%.C0、C1(/mL),A0、A1.1.3 ,,(4000r/min、30min),,,3,.50mL12mL(),,,3min,30min,10min,,A,.2 2.1 ,.,().3:、.3,5,,,(1).1,---,1b,30min,;--,,,-.-50min,30min99.7%,92%30%,.,.、、,(2),、,-,.,、,、,.1 2 33 2.2 (1).,1.0(),(1).3.3:、60%～80%9%～16%,25%40%.A0.3～0.7,3.2%6.8%.-,[8].,,,A0.5±0.2.(2)、.,.12mL、(),0.6mL,1.5mL,3.0mL,4.5mL、6.0mL、8.0mL、10.0mLCCl4.4.4,12mL,(CCl4)4.5mL,(),.,,,CCl4,,,、2.67(:=12mL∶4.5mL).(3).CCl4,30min,.,.,1000r/min·12· 254 CCl4.,,,.t,(,):,A1A2;,(-)A3,,A4.1.1 t/minA1A2A3A420.3040.3040.3210.31640.3030.3040.3180.31060.3040.3030.3220.30880.3030.3010.3160.29390.3040.3020.3170.285t/minA1A2A3A4100.3050.3030.3240.273110.3060.2910.3210.261120.3050.2860.3180.234130.3050.2720.3190.2151000r/min,10min,,,(1),,,,,.,,:CCl4,,,;,.,,-,,10min.,1000r/min、10min.2.3 ,()Ax=0.387()Af=0.713,5,2.0.5%1.6%,,.2 ()(A)/%(A)/%0.03192.00.63810.50.02892.80.63311.20.03491.20.64010.3(A)/%(A)/%0.03192.00.63610.80.02992.50.64010.3 0.5% 1.6%5 、2.4 -,(、),5,80%,20%.(4.0cm-1,20),(cm-1),(%).6、3.6,、、,1643cm-1,,.3670～3200cm-1,,,-OH-NH.,,.·13·1,:-3 [9]/cm-13670～3200,-OH、-NH2-NH.2360～2070-C≡C-、-C≡N--N≡N-1643,-COO、C=C-NO2,-CH2.()900-(CH2)n-6 3 、,.、,-,..,(0.5±0.2);、2.67;(1000r/min、10min).(80%),(20%).:[1] HermanssonM,JlangQ,GotschalkBW.Hydrophobicandelectrostaticcharacterzationofbacteriaanditsrelationshiptoadhensiontoanair-waterinterfaces[J].ArchMicrobiol.1983,131:308-312.[2] vanLoosdrechtMCM,LyklemaJ,NordeW,etal.Theroleofbacterialcellwallhydrophobicityinadhension[J].ApplEnvironMicrobiol.1987,53(8):1893-1897.[3] DevasiaP.SurfacechemistryofThiobacillusferrooxidousrelevanttoadhensiononmineralsurfaces[J].ApplEnvironMicrobiol.1993,59(12):4051-4055.[4] RosenberyM,KjellebergS.Hydrophobicinteractions:roleinbacterialadhension[M]//Marshall,K.C.Ad-vancesinMicrobialecology.NewYork:PlenumPress,1986:353-393.[5] ,,,.[J].,2004,29(5):585-589.[6] RosenbergM,GutnickD,RosenbergE.Adherenceofbacteriatohydrocarbons:asimplemethodformeasuringcell-sur-facehydrophobicity[J].FEMSMicrobiolLett.1980(9):29-33.[7] ,,,.[J].,1999,5(5):491-495.[8] .[M].:,1996:340-350.[9] .:3[M].:,2004.Extraction-turbidimetricmeasuringhydrophobicmicroorganismincokingwastewaterZHENGXi-qiang,CAIChang-feng,LINa(Dept.ofBioch.Engn.,AnhuiUniversityofTechnologyandScience,Wuhu241000,China)Abstract:Aftercompareingthreekindsofextractingagents,selectingoptimumbacilliandthebestratioofex-tractingagents,theoptimumseparationconditionsweredetermined.Theresultsshowthatcapacitiesofcarbontetrachloride'sadsorbingmicro-organismswerehigh,andmicro-organismscouldmaintainstabilityintheinterfaceofcarbontetrachloride-waterandthecarbontetrachloridelayerforalongtime.Bykeepingtheconcentrationoftestmicro-organismsalmostconstant,thebestratioofbacilliandextractantwas2.67(bacilli:extractant=12mL∶4.5mL).Usinglow-speedcentrifugation(1000r/min、10min)asseparationconditions,mainmicrobialhy-drophobicintheactualcokingwastewaterwasdetermined.Moreover,theinfraredspectraofmicroorganismshasbeenanalyzed,showingthatmethodissimpleandfeasible.Keywords:cokingwastewater;microorganism;extractingagent;ratioofhydrophobic·14· 25