大庆湿地水体中可培养蓝藻的分布及其形态鉴定

5。[1]、[2]、[3]、[4-5]、。[6-8]。。[6-11]。123°45′～125°47′E45°23′~47°28′N。60163319。。4Chroococcales、Rivulariales、OscillatorialesNostocales8Synechococcus、Synechocystis、CalothrixLyngbya、Symploca、Microcoleus、PhormidiumAnabaena1Gloeocapsagroup。Q938.2A1002-2090201605-0077-04DistributionandIdentificationofCultivableCyanobacteriafromWaterSampleinDaqingWetlandXieLingnanWangXinleiWangLiyanJinLeiZhangWenjinJingRuiyongLiuYongchunCollegeoflifeScienceandTechnologyHeilongjiangBayiAgriculturalUniversityDaqing163319Abstract:CultivableCyanobacteriawasenrichedbylimited-culturetechnologythecultivablecyanobacteriainDaqingwetlandwasobservedandidentifiedbymicroscope.TheresultsshowedthattherewereunicellularandfilamentouscultivablecyanobacteriainDaqingwetlandcultivablecyanobacterialspeciesweredifferentindifferenttypewetlandsatdifferentsamplestages.Bytheidentificationofmicroscopicobservationtheobservedcyanobacteriaweredistributedinto4ordersofchroococcalesRivularialesOscillatorialesandNostocales8genusofSynechococcusSynechocystisCalothrixLyngbyaSymplocaMicrocoleusPhormidiumAnabaenaandGloeocapsagroup.KeywordsCynaobacteriaenrichmentmicroscopicobservationDaqingwetlanddistribution2015-12-18xc2014061201410223024XDB2014-1331300425。1994-2012。[e-mail]。doi:10.3969/j.issn.1002-2090.2016.05.015285201610JournalofHeilongjiangBayiAgriculturalUniversity28577~80Oct.201628、、、[12]。。[6-7][10]。[9]。11.12014512、612N46°35′E125°05′、N47°18′E124°25′、N47°18′E124°26′、N46°32′E125°11′、N47°18′E124°37′620、N46°35′E125°07′。5cm76h4℃。1.220145mL45mLBG-11[11]100mg·mL-1250mL25℃2000lx。4~5。10%v/v。55。1.310μLOlympus40100。、、、《》[10]Waterbury《》[11]。22.16ZLTZLW、LFWLWBLLMLDP。BG-115。1、。、、Waterbury《》ZLW、LWBZLTGloeocapsagroup、SynechococcusSyne－chocystis1。《》[10]1、13Rivulariales、OscillatorialesNostocales6CalothrixLyngbya、Symploca、Micro－coleus、PhormidiumAnabae－na。SynechococcusCalothrix、LyngbyaSynechocystis、GloeocapsagroupLyngbya、Symploca、Micro－coleus、PhormidiumAnabaena。2.24。1、、40d。SynechococcusLyngbyaSynechocystisSymplocaMicrocoleus。。7851Table1MorphologicalindexdetectionofcyanobacteriaindifferentwatersamplesofDaqingwetlandILWB-May1-1IILWB-May1-2IIILWB-Jun12-1IVLLM-May1-1VZLT-May2-1VIZLT-May2-2VIIZLT-Jun12-1VIIIZLT-Jun12-2IXZLW-May2-1XZLW-May2-2XIZLW-Jun12-1XIIZLW-Jun12-2XIIILFW-May1-1XIVLFW-Jun12-1XVLFW-Jun12-2XVILDP-Jun20-1--。1Fig.1MorphologicalmicrographofcultivablecyanobacteriafromdifferentwatersamplesinDaqingwetland
IIIIIIIVVVIVIIVIIIIXXXIXIIXIIIXIVXVIXVIIIIIIIVVVIVIIVIIIIXXXIXIIXIIIXIVXVIXVLWB-May1-1LWB-May1-2LWB-Jun12-1LLM-May1-1ZLT-May1-1ZLT-May1-2ZLT-Jun12-1ZLT-Jun12-2ZLW-May2-1ZLW-May2-2ZLW-Jun12-1ZLW-Jun12-2LFW-May1-1LFW-Jun12-1LFW-Jun12-2LDP-Jun20-1IIIIIIIVVVIVIIVIIIIXXXI:XIIXIIIXIVXVXVI/μm1.1~3.1×0.5~1.14.9~5.5×1.1~1.62.0~4.2×0.7~1.22.1~6.5×0.4~0.92.1~3.6×2.1~3.22.8~4.2×0.5~0.91.7~2.7×1.8~3.12.3~3.87.2~9.1×7.3~9.51.0~2.0×1.1~1.62.1~4.4×0.9~1.43.3~8.4×0.9~1.71.8~2.5×0.6~0.91.4~2.3×0.3~1.01.7~3.1×0.6~1.13.5~6.6×0.8~1.3+-----+-------+-------------++---+++-+++-+++++++、、、、、SynechococcusCalothrixLyngbyaLyngbyaSynechocystisSymplocaCalothrixMicrocoleusGloeocapsagroupPhormidiumLyngbyaLyngbyaAnabaenaAnabaenaCalothrixCalothrix+-。79283[10,13]。[11]DNA[13-14]。[5-7]。。Prochloro－coccusSynechococcus[15]。[13]。5～9μm。。。6BG-11Chroococ－calesSynechococcus、Syne－chocystisGloeocapsagroup。RivularialesCalothrix、Oscil－latorialesLyngbya、Symploca、MicrocoleusPhormidiumAnabaena。。。1.J.198212339-42.2.J.201030101992-1998.3.J.20039128-31.4.J.201020427-30.5.C//.2007:12-15.6,.J.,20151:75-79.7.J.201036104-7.8ChangFH.CellmorphologyandlifehistoryofDictyochaoctonariaDictoyochophyceaeOchrophytafromWelling－tonharbor,NewZealandJ.PhycologicalResearch2015634253-264.9ThajuddinNMuralitharanGSundaramoorthyMetal.Morphologicalandgeneticdiversityofsymbioticcyanobac－teriafromcycadsJ.JournalofBasicMicrobiology2010503254-265.10.···M.2007.11WaterburyJB.Thecyanobacteria-IsolationpurificationandidentificationM.Prokaryotes20061053-1073.12.J.201527269-73.13.PCR-DGGEJ.2015252155-172.14ChonudomkulDYongmanitchaiWTheeragoolGetal.MorphologygeneticdiversitytemperaturetoleranceandtoxicofCylindrospermopsisraciborskiiNostocalesCyanobacteriastrainsfromThailandandJapanJ.FEMSMicrobiologyEcology2004483345-355.15PartenskyFHessWRVaulotD.Prochlorococcusama－rinephotosyntheticprokaryoteofglobalsignificanceJ.MicrobiologyandMolecularBiologyReview1999631106-127.80