搜索
38220172ENVIＲONMENTALSCIENCEVol．38No．2Feb．2017A/ON2O710021SBＲA/O、HＲT、SＲT、、A/ON2O．A/OA/OCOD、95%TN72.1%52.2%．3hA/OA/ON2O16.95mg3.59mgN2OTN11.47%4.11%N2ON2O．A/OA/OAOB、NOBAOBNitrosomonasA/ONOBA/O．A/ON2O．A/OA/ON2OX703.1A0250-3301201702-0647-07DOI10．13227/j．hjkx．2016031522016-03-202016-09-072016JM507914JK1096BJ13-081968～E-mailguochangzi@sust．edu．cnNitrogenＲemovalEffectandConversionCharacteristicsofNitrousOxideinSingle-stageandMulti-stageA/OProcessesGUOChang-ziZHANGFeng-yanLIUFu-yuZHUChaoPEILi-yingSchoolofEnvironmentalScienceandEngineeringShaanxiUniversityofScienceandTechnologyXi'an710021ChinaAbstractThesingle-stageA/Oandmulti-stageA/OprocessesweresimulatedbysequencingbatchreactorsSBＲswithalternatestirringandaeration．TheremovalefficiencyofnitrogenandthereleasemechanismofN2OwerestudiedundertheidenticalconditionsofinfluentqualityhydraulicretentiontimeHＲTsludgeretentiontimeSＲTtemperatureandanoxic/oxicA/Oretentiontimeratio．ExperimentalresultsshowedthatCODorammonia-nitrogenremovalhadnosignificantdifferencebetweenthesingle-stageandthemulti-stageA/Oprocessesfortheinfluentqualityequivalenttomunicipalwastewater．HoweverTNremovalefficiencyoftheformerwasbetterthanthelaterwith72.1%and52.2%respectively．Intheconversionoftotalnitrogenduringthetypicalcycleinthesingle-stageA/Oandmulti-stageA/OprocessestheyieldsofN2Owere16.95mgand3.95mgrespectively．TheconversionratewhichistheratioofN2OyieldandTNremovalwasrespectively11.47%and4.11%．N2OproductionandemissionoccurredmainlyinaerobicnitrificationphasewhiletherewaslittleN2Oemissioninanoxicdenitrificationphase．AlthoughthedominantspeciesofAOBwasbothNitrosomonasinthesingle-stageA/Oandthemulti-stageA/OprocessesunderthesameoperatingconditionsitwasmoreconducivetothegrowthofnitrifyingbacteriaAOBNOBinthesingle-stageA/OprocesswiththegreaterabundanceofNitrosomonas．MeanwhilethetypeandabundanceofNOBinthesingle-stageA/Oprocessweresignificantlymorethaninthemulti-stageA/Oprocesstoo．Thereforeitismorecompetitivetodealwiththehigh-strengtheningammonia-nitrogenwastewaterinthesingle-stageA/Oprocess．IntheactualoperationofwastewatertreatmentusingappropriatepartitionsofA/Ooroxygen-supplyingmodescannotonlyresultinbetternitrogenremovalbutalsodecreasethesecondarypollutioncausedbyN2Ototheatmosphere．Keywordssingle-stageA/Omulti-stageA/OnitrogenremovalnitrousoxideN2ONitrosomonas．N2N2O12．N2OCO2270150a34．N2ON2O．N2O、DO、pH38N2O5～8．A/ON2O．A/OA/OSBＲ、CASS、A/O、A2/OA/O、biloakA/O9～12．A/ON2O、．11.1A/OA/OSBＲ．11215L3h140min35min5min．1A/O70min2min70min．2A/O140min546min1min．1728min4A/O13．220A/O．1270min．HＲT=12h、SＲT=30d、MLSS=2300±100mg·L－125℃．1Fig．1Schematicdiagramofexperimentdevices1.2COD300mg·L－1NH4ClN40mg·L－1KH2PO4P6mg·L－1NaHCO3470mg·L－11mg·L－1CuCl2·2H2O35mg·L－1NiCl·6H2O36mg·L－1MgSO4·7H2O5000mg·L－1FeCl2·4H2O6000mg·L－1CoCl2·4H2O880mg·L－1ZnSO4·7H2O100mg·L－1MnCl2·4H2O500mg·L－1．1.314．1.4SBＲ110min27min．1.4.1DO、pHDO、pHHANNApH/OＲP．COD、NH+4-N、NO－3-N、NO－2-N、TN、TPSV、MLSS、SVI．15．1.4.2N2O24h．N2OGC-ECDPE-600PoropakQ30mL·min－1、50℃、50℃、360℃16．1.4.3SoilGenDNAKitDNAPCＲDNAＲoche454GSFLX12DNA1718．．MEGANA/O．22.1A/ODOA/OCOD、、、2．8462A/ON2O2COD97.5%99.2%．1272.1%52.2%53.4%、25.2%．Guo193Fig．3TimeprofilesofDOconcentrationinatypicalcycle2A/O、A/OA/O．3DOA/O70min．1DO“”DO2A/OFig．2PollutantremovalperformanceunderdifferentA/OconditionsDO．2DO“”2.78mg·L－11DOCOD．SOUＲMLSS12SOUＲ24.71mg·g·h－128.64mg·g·h－1A/O．2.24a1NH+4-N、NO－2-NNO－3-N．NH+4-N．NO－3-N．4aNO－2-NNO－2-NNO－2-NFAAslan20FAAOBNOBAOBNOB10～150mg·L－10.1～1.0mg·L－1．15minFA1.07mg·L－1NOB．4b2NH+4-N、NO－2-NNO－3-N．17NH+4-N．FA0.1mg·L－1FANOB．NO－2-N．NO－3-NNO－3-N．2.3N2ON2O946384NH+4-N、NO－2-NNO－3-NFig．4TimeprofilesofNH+4-NNO－2-NandNO－3-NconcentrationsinatypicalcycleunderdifferentA/OconditionsN2O99.5%21．N2ON2O．5a1N2O．N2OvN2OcN2ODODON2O．N2ON2O．N2OvN2OMLSScN2O100min5μg·g·min－17.38μg·L－10.58mg·L－1N2O．5b2N2OvN2OcN2OvN2O1.8μg·g·min－1．vN2O1．5N2OFig．5TimeprofilesofN2OproductionandreleaseinatypicalcycleunderdifferentA/OconditionsN2O12．12N2O16.59mg3.95mgN2OTN11.47%4.11%．A/ON2OA/O4.2．Hu22COD/NN2OCOD/N7.514.5N2O6%1.3%AOBNitrosomonas．2.4A/OPCＲ-T-ＲFLP6．．612AOBNitrosomonas1NOBNitrobacter0562A/ON2O126Fig．6Evolutionbranchesofthemicroorganismsintworeactors11N2OTable1N2OproductionandconversionrateofNo.1reactorTN/mgTN/mgTN/mgN2O/mgN2O/%251.25181.569.750.530.75181.5112.868.715.3522.35138.4516.5911.47Nitrospira2．2NO－22NO－2NOBAOBnitrifier'sdenitrificationND2N2O23．2.22NO－2-NNO－2．1563822N2OTable2N2OproductionandconversionrateofNo.2reactorTN/mgTN/mgTN/mgN2O/mgN2O/%184.91156.8428.070.853.03187.06171.2315.930.794.96206.92189.5417.380.804.60228.06212.6115.450.774.98251.51232.3519.160.743.8696．193.954.111NOBNitrobacterNitrospira1NO－22NOB．2.21NO－2-NNOBNitrobacterNitrospira2425．1NO－22NOB．1AOBN2O．26～28AOBN2OAOBNitrosomonas．Nitrosomonas．Shrestha29Nitrosomonaseuropaea7%N2O．1Nitrosomonas2N2O．A/OAOB、NOBN2O．A/O-A/ON2O．31A/OA/OCOD、A/OA/O．HＲT=12h、SＲT=30d、25℃72%TN=7.79mg·L－152%TN=15.58mg·L－1．2A/ON2OA/O4.2A/OA/ON2O11.47%4.11%．N2OAOBNitrosomonas．3A/OA/OAOB、NOB．AOBNitrosomonasA/ONitrosomonasA/ONOBNitrobacterNitrospiraA/O．1．N2OJ．20103091729-1738．GengJJWangYYZhangZXetal．N2Oproduc