单级自养脱氮工艺氨氮去除途径研究杨国红

,＊,,,(,　400045):SBBR,,,.,,,62%NO-2、NO-3、NH2OH、N2H4、NO、NO2、N2ON2,N290.07%..4.5%,3.73%,53.77%,,2.NO2,,NH+4NO2NO-2N2,.:;;;;:X703.1　:A　:0250-3301(2009)01-0102-06:2008-04-14;:2008-08-04:(50608071);(308020);(200801A1A0180277):(1981～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]movalintheCompletelyAutotrophicNitrogenRemovalinOneReactorProcessYANGGuo-hong,FANGFang,GUOJin-song,QINYu,WEIYing(KeyLaboratoryoftheThreeGorgesReservoirRegion'sEco-Environment,MinistryofEducation,ChongqingUniversity,Chongqing400045,China)Abstract:Differentsyntheticwastewaterswereusedinthebatchteststoanalyzetheintermediateproductsandthenitrogenbalance,andtostudythemechanismofammoniumremovalinthecompletelyautotrophicnitrogenremovalinonereactorprocesswiththesludgeculturedintheSBBRcompletelyautotrophicnitrogenremovalsystem.Theresultsshowedthat62%ofammoniumwasconvertedtosuchnitrogencompoundsasNO-2,NO-3,NH2OH,N2H4,NO,NO2,N2OandN2withoutadditionoforganiccarbon,andN2tookup90.07%.Theammoniuminthecompletelyautotrophicnitrogenremovalinonereactorsystemwasremovedinmanyways.4.5%ofammoniumwasremovedinthephysical-chemicalway.3.73%ofammoniumwasconvertedbytheconventionalnitrification-denitrificationprocess.Thequantityofammoniumremovedbythecompletelyautotrophicnitrogenremovalinonereactorprocesswas53.77%,whichisthelargest,andthecompletelyautotrophicnitrogenremovalinonereactorprocesscouldberealizedintwodifferentmetabolicpathways.Buttheeffluentammoniumintheanoxicreactor,whereenoughNO2presentwerepresent,wasequaltotheblanksystem,andnoammoniumwasconvertedtosuchnitrogencompoundsasNO-2andN2byNitrosomonaseutrophausingNO2aselectronacceptor,whichmaybecausedbylackofthefunctionbacteria.Keywords:completelyautotrophicnitrogenremovalinonereactor;ammonium;metabolicpathway;anaerobicammoniumoxidation(ANAMMOX);nitrification-denitrification　　,,10[1～3],、、CN[4,5].,,.NH+4NO-2,NO-2NH+4N2[6,7].,,.,1[8].NOx.,NO2NH3NO、NO-2,NO-2N2N2O.NO2N2O4※NO※NO2※N2O4[9,10].30120091　　　　　　ENVIRONMENTALSCIENCEVol.30,No.1Jan.,2009DOI:10.13227/j.hjkx.2009.01.043,,,,.1　[8]Fig.1　MetabolicpathwayofANAMMOXstudied[8]　1　1.1　,500mL.,30℃130rmin,30℃,.,(,4L)NO、NO2、N2ON2.,30℃,Ar30min,N2O2,DOLDOTMHQ10(0.01mgL).Ar30min,,.12h,3,(,).,NH+4、NO-2、NO-3、NH2OH、N2H4TN.,3,0.5h1,1.1.2　,(1),NaHCO3pH8.0±0.2,(EDTA5.0gL,CoCl2·6H2O1.6gL,ZnSO4·7H2O2.2gL,MnCl2·4H2O5.1gL,CuSO4·5H2O1.6gL,(NH4)6Mo7O24·4H2O1.1gL,CaCl2·2H2O5.5gL,FeSO4·7H2O5.0gL).,1.O2ArO220%,Ar80%.NO2ArNO22%,Ar98%.4～6mLmin.1　Table1　Wastewaterandgasusedintheexperimentmg·L-1ⅠNH4HCO3(N,)30O2ArⅡNH4HCO330NO2ArⅢNH2OHCl30ArNaNO230ⅣNH4HCO330O2ArCH3OH(C,)15ⅤKNO330ArCH3OH15ⅥN2H4·H2O30ArⅦNH4HCO330ArNH2OHCl30ⅧNaNO230Ar1.3　2SBBR[11,12],(MLSS)873.6mgL.1.4　NH+4:[13];NO-2:N-(1-)-[13];NO-3TN:-[13];COD:-;MLSS:[13].NH2OHNH2NH2[14,15].NONO242CNO-NO2-NOxAnalyzer(ThermoScientific,).N2OECD5890(,).N2TCDSC-6000(,).pH:Sension2pHISE(Hach1031:,);:LDOTMHQ10(Hach,).2　2.1　Ⅰ,300mL,O2Ar,4～6mLminDO1.0(±0.2)mgL,.12hNO、NO2、N2ON2,2.2　Table2　ConversionofammoniuminthecompletelyautotrophicnitrogenremovalinonereactorNH+4inNH+4NO-2NO-3NH2OHN2H4NONO2N2ON2(N)mg·L-130.0011.400.0980.620.880.00025.97×10-41.67×10-47.10×10-32.02mL3003003003003003002247224722472247(N)mg9.003.420.0290.190.2640.000060.00130.000380.0164.54　　,12h28.75mgL,NH3,0.375mg,NH3,:NH+4in=NH+4+NO-2+NO-3+NH2OH+N2H4+NO+NO2+N2O+N2+NH3+Δx,Δx,Δx=0.16426mg.2.2　1,,,,.,3.Ⅱ,NO2Ar,DO,12h28.83mgL,　　　,N2(3),0.35mgNH+4-NNH3NO2Ar,.NO2,NO2NO、NO-2N2,Schmidt[9,10],.NH2OHClNaNO2Ⅲ,Ar.12h0.91mgNH2OH(N,),NO、NO2、N2ON2,N2ON2,17.14%82.64%.2h1,2.NH2OHNO-2,NH2OH,NH2OH.NO-3,0.15mgL.3　1)mgTable3　EffluentquantityofnitrogencompoundsinthecompletelyautotrophicnitrogenremovalsystemwithdifferentinfluentwastewatersmgNH+4NO-2NO-3NH2OHN2H4NONO2N2ON2NH4HCO33.420.0290.190.2640.000060.00130.000380.0164.54NH4HCO3NO28.65—0.06———0.00076—NH2OHClNaNO22.990.090.910.0130.00281.235.93NH4HCO3CH3OH2.670.0020.05KNO3CH3OH1.795.270.00390.0000660.00261.96N2H4·H2O2.24———5.58NH4HCO3NH2OHCl6.260.027—5.182.01NaNO25.570.051.681)“—”,N104　　　　　　302　ⅢFig.2　ConversionofnitrogencompoundswithsyntheticⅢasinfluentwastewater　　　Ⅵ、ⅦⅧ,Ar,1.3,NaNO2,12h1.68mgNH2OH-N,NO-3,NO-2(3.43mgL)NH2OH.　　　NO-2、NO-3NH2OH3(a),NO-2[0.97mg·(L·h)-1,N,]NH2OH[0.45mg·(L·h)-1]2.16,NO-3,0.15mgL;NH4HCO3NH2OHCl,N2H4,13.34mgL,NO-2,NO-3,NH+4NH2OH(6.56mg)N2H4NO-2(4.02mg),.NH+4、NH2OH、N2H4NO-23(b),NH+4NH2OH,,NH2OH;N2H4,N2H47.47mgL,5.58mgN2,NO、NO2N2O.N2H43(c),N2H4,1.99mg·(L·h)-1.3　Fig.3　Conversionofnitrogencompoundswithdifferentinfluentwastewaters　　　,,,.,KNO3CH3OH,.12h,NO-330mgL17.56mgL,COD60mgL57.51mgL,5.97mgLNO-2,NO、NO2、N2ON2,N2(3),,.,4.,CH3OH,COD,12hCODCOD2.55mgL,CH3OHNO-2NO-3CH3OH,CODNO-2NO-3.CH3OH,9.8%3.73%.1051:4　Fig.4　Conversionofammoniumwithandwithoutadditionoforganicmattersinthecompletelyautotrophicnitrogenremovalsystem　3　,,62%NO-2、NO-3、NH2OH、N2H4、NO、NO2、N2ON2,N2,90.07%,.,,.4,3.73%,,3.73%.Helmer[16]18%.,4.5%.53.77%.,.,,.2,NH+4,NO-2,.32,NH2OHClNaNO2,NH2OHNO-2,N2ON2.3KNO3CH3OH,,21.78%NO-3N2,1.79mgNO-2,,,,NO-2.NH2OH,,5,NH+4NH2OH,NH2OHNO-2N2O,N2ON2.Wood[17]Thauer[18],NH3NO-2N2ΔG0=-358kJ·mol-1,NH3NH2OHΔG0=-87kJ·mol-1,NH2OH