氮磷对铜绿微囊藻群体形态的影响

2711201411ＲesearchofEnvironmentalSciencesVol．27No．11Nov．2014．J．201427111251-1257．ZHOUJianYANGGuijunQINBoqiangetal．EffectsofnitrogenandphosporousoncolonyformationofMicrocystisaeruginosaJ．ＲesearchofEnvironmentalSciences201427111251-1257．[e-mail]．*[e-mail]1231*331．2100082．1000393．21412210～103．〔25±1℃2000lx12h∶12h〕ρTN、ρTPBG11FACHB912T1ρTN、ρTP1.00、0.05mgLT25.00、0.25mgLT325.00、1.25mgLT4125.00、6.25mgLT5247.06、7.11mgL．T1、T2T3T4T5T1、T2、T3T4、T5P＜0.05．T1、T2、T3＞100T2960T4、T5．ρTN、ρTPρTN、ρTP．、X1731001－6929201411－1251－07ADOI10.13198j．issn．1001-6929．2014．11．05EffectsofNitrogenandPhosporousonColonyFormationofMicrocystisaeruginosaZHOUJian12YANGGui-jun3QINBo-qiang1*ZHANGGuang-sheng3XUHui-ping31．StateKeyLaboratoryofLakeScienceandEnvironmentNanjingInstituteofGeographyandLimnologyChineseAcademyofSciencesNanjing210008China2．UniversityofChineseAcademyofSciencesBeijing100039China3．SchoolofEnvironmentalandCivilEngineeringJiangnanUniversityWuxi214122ChinaAbstractItiswidelyrecognizedthatacolonyofMicrocystiswithseveralhundredsoreventhousandsofcellsinafieldwillbereplacedbyasinglecellandafewpairedcellsafterseveralgenerationsinaxeniclaboratorycultures．MicrocystisaeruginosawerecollectedfromLakeTaihutoconductalaboratoryexperiment25±1℃2000lx12h∶12hwithdifferentlevelsofρTNandρTPofblue-greenmediumBG11．TheρTNandρTPofT1T2T3T4T5were1.00and0.05mgL5.00and0.25mgL25.00and1.25mgL125.00and6.25mgL247.06and7.11mgLBG11respectively．DuringtheexperimentthecolonysizeandtheproportionofcellsincolonialformofM．aeruginosaincreasedinT1T2andT3butdecreasedinT4andT5．AsignificantdifferencewasfoundbetweenthetreatmentsofT1T2andT3andthetreatmentsofT4andT5P＜0.05．Colonysizesoverthan100cellswereobservedinT1T2andT3．ThecolonydevelopedbestinT2andthelargestcolonywasabout960cellspercolony．IncontrastcolonyformationwasentirelyabsentinT4andT5．ThecolonyformationofM．aeruginosaabsorbedmorenutrientsandhadhighercellgrowthinlowρTNandρTP．HoweverhighρTNandρTPthatprovideawealthofnutrientsmayinhibitornotstimulatethesynthesisandsecretionofextracellularpolysaccharidesofM．aeruginosacellsandthisisadisadvantagetocolonyformationmaintainance．27KeywordsMicrocystisaeruginosacolonyformationcolonydisappearnitrogenandphosphorusconcentration1．23．．10～103456．．7．．、468、9-10、11-13、14-15、16-17．N、P18．ρTN、ρTP19-23〔ρTNρTP〕24-27．、23．WANG28ρTNρTN．、、29-30．28．ρTN、ρTP．BG11blue-greenmediumρTNρTP247.067.11mgLρTN、ρTP2.9mgL1.1～10.9mgL0.23mgL0.08～2.13mgL31．ρTN、ρTP．MA32N、PρTN、ρTP．ρTN、ρTP．ρTN、ρTP．11.1FACHB912．ρTN、ρTP10.00、0.50mgLBG1125±1℃2000lx12h∶12h．44.91%55.09%．3～10＞10～10025.62±0.1621.50±1.47．1.2BG11ρTN、ρTP3351．NaNO3KH2PO4NP．T1、T2、T3T4N、PBG11NaNO3KH2PO4ρTN、ρTPT5BG11100mL250mL．10mL、1ρTN、ρTPTable1ConcentrationsofTNTPandtheirratiointhefivetreatmentsρTNmgLρTPmgLT11.000.0520.00T25.000.2520.00T325.001.2520.00T4125.006.2520.00T5247.067.1134.752521113.92×108L－1．．1.33201253—2421d．900．OlympusCKX31400×3d．2%．．3～9603Ⅰ3～10Ⅱ＞10～100Ⅲ＞100．5050．μ=lnX2X1t2－t1μd－1X1X2t1t2L－1t2－t1d．1.4ANOVA、．SPSS18.0Origin8.5．22.1ρTN、ρTP1T1、T2、T3T2T4、T5．115Fig.1PicturesofM．aeruginosacoloniesindifferenttreatmentstookinday15duringtheexperiment32．2T1、T2T3ⅠT4T521T1、T2、T3、T4T5Ⅰ5.57±0.74、5.30±0.57、5.54±0.51、3.25±0.073.60±0.16．T1、T2T3ⅡT4、T51812T1、T2T3Ⅱ23.23±1.33、32.33±4.3028.43±3.33．ⅠⅡT1、T2、T3T4、T5P＜0.01．Ⅲ6、6、3T1、T2T3T4、T5Ⅲ．ⅢT1、T2T321T1ⅢT1、T2T3Ⅲ3521272Fig.2ChangesincolonysizeofM．aeruginosaindifferenttreatmentsduringtheexperiment209.60±29.09、372.70±41.76259.00±35.28P＞0.05．2.2ρTN、ρTPP＞0.05．3T1、T2、T3T4T5ⅠT1、T2、T3T4、T5P＜0.05．T1T2T3、T4、T5T4、T5．3Fig.3Changesindensityofdifferentcell-sizeunitsofM．aeruginosaindifferenttreatmentsT1、T2T3T4、T54．T1、T2、T3、T4T582.809%±6.242%、84.344%±6.561%、67.041%±3.777%、0.003%±0.000%7.674%±1.024%．μ5．55．9ρTN、ρTP9．P＞0.05．4521114Fig.4Changesinproportionofdifferentcell-sizeunitsofM．aeruginosa5Fig.5GrowthratesofM．aeruginosaindifferenttreatments3ρTN、ρTP．ρTN、ρTP〔ρTN≤25.00mgL、ρTP≤1.25mgL〕ρTN、ρTP〔ρTN≥125.00mgL、ρTP≥6.25mgL〕P＜0.05ρTN5.00mgL、ρTP0.25mgL．ρTN、ρTPρTN、ρTPP＜0.05．348ρTN、ρTP．2034-38EPS．N、P3940．Moreno16Anabaenasp．ρTN．41-43NP4244．45NP．46ρTN≤1.98mgL、ρTP＜0.65mgLBG11ρTP＞0.65mgL．package-effect46．、46-47．、．ρTN、ρTPT4、T5T4T5552127．ρTN、ρTPT1、T2、T3T1、T2T3．．T1N、P21Ⅲ．48-50PCuPm、ETＲmax4850．、．ρTN、ρTPρTN、ρTPT1、T2、T39ρTN、ρTP9ρTN、ρTP．BG1134.7420.00T4T5．4aρTN、ρTP〔ρTN≤25.00mgL、ρTP≤1.25mgL〕ρTN、ρTP〔ρTN≥125.00mgL、ρTP≥6.25mgL〕ρTN5.00mgL、ρTP0.25mgL．bρTN、ρTP〔ρTN≤25.00mgL、ρTP≤1.25mgL〕ρTN、ρTP〔ρTN≥125.00mgL、ρTP≥6.25mgL〕．cρTN、ρTPρTN、ρTP．Ｒeferences1PAEＲLHWOTTENTG．HarmfulcyanobacterialbloomscausesconsequencesandcontrolsJ．EnvironmentalMicrobiology201365995-1010．2WYNNETTSTUMPFＲPTOMLINSONMCetal．CharacterizingacyanobacterialbloominwesternLakeErieusingsatelliteimageryandmeteorologicaldataJ．LimnologyOceanography2010552025-2036．3QINBoqiangZHUGuangweiGAOGuangetal．AdrinkingwatercrisisinLakeTaihuChinalinkagetoclimaticvariabilityandlakemanagementJ．EnvironManage201045105-112．4BUＲKEＲTUHYENSTＲANDPDＲAKAＲESetal．EffectsofthemixotrophicflagellateOchromonassp．oncolonyformationin