氮素负荷及高温冲击对UASBANAMMOX反应器的运行影响孙艳波

CHEMICALINDUSTRYANDENGINEERINGPROGRESS2009289·1672·UASB-ANAMMOX5100063.2L-UASB-ANAMMOXANAMMOXUASB-ANAMMOXDONRANAMMOXX703A10006613200909167205InfluenceofnitrogenloadingandhightemperatureshockforrunningofUASB-ANAMMOXreactorSUNYanboZHOUShaoqiLIHuoshengYANGZhiquanCollegeofEnvironmentalScienceandEngineeringSouthChinaUniversityofTechnologyGuangzhou510006GuangdongChinaAbstractTheinfluenceofnitrogenloadingandhightemperatureshockonANAMMOX(anaerobicammoniumoxidation)processwitha3.2LUASB(up-flowanaerobicsludgeblanket)reactorwasinvestigated.TheresultsshowedthattheUASB-ANAMMOXreactorisnotonlyappropriateforthetreatmentoflowammoniacontainingwastewater,butalsosuitableforthatofmediatetohighammoniacontainingwastewater.Thenitritereductaseismorelikelysubjectedtotheinhibitionofdissolvedoxygen.Ainstanthightemperaturemaygiveaheavyshockontherunningofthereactor;however,theshockmayberelievedbecauseofanintrinsicmechanismcausedbytheANAMMOXmicrobes,whichresultedintheresilienceofthereactortoasteadystate.KeywordsANAMMOXnitrogenloadingdissolvedoxygenUASBANAMMOXNH4+NO2NO3NH4+NO2NO3N2[1]A/OA2/OSBR[13]ANAMMOX[112]ANAMMOX[1012]ANAMMOXDOHRTpHORP[8,13]UASB-ANAMMOX4UASB-ANA[e-mail]:10.16085/j.issn.1000-6613.2009.09.0289UASB-ANAMMOX·1673·11.11UASBANAMMOX313.2L2.28L0.92LUASB(32±1)12346578999910121111234567891011121.2(NH4)2SO4NaNO2NaHCO3KH2PO4g/LEDTA5.000FeSO45.000g/LZnSO4·7H2O0.430CuSO4·5H2O0.250MnCl2·4H2O0.990NiCl2·6H2O0.190CoCl2·6H2O0.24H3BO40.0141.0mL/LpH7.98.21.3SBR31.4[14]NH4+-NNO2-NN-(1-)-pHPHS-9V1.51202.0L/dNH4+-NNO2-N411/d/kgTN·(m3•d)1ρ1ρ2(1300.1360.14211.3231600.2020.21411.1661990.2550.28011.10()1001200.0990.11111.32ρ1NH4+-Nρ2NO2-N32434h32322.10.06kgTN/(m3•d)()2NH4+-NNO2-N0.061kg/(m3·d)0.077kg/(m3•d)()NH4+-NNO2-N99.8%NH4+-NNO2-NTN85%87.2%310.095kg/(m3•d)0.110kg/(m3•d)404592%95%TN85%404589.85%79.02%3840200928·1674·0.150.120.090.060.030.00020406080100020406080100120t/d/kg·m3·d1/%a0.150.120.090.060.030.00020406080100020406080100120t/d/kg·m3·d1/%b0.350.250.200.150.050.00020406080100020406080100120t/dTN/kg·m3·d1/%0.100.30c2ANAMMOX3NO2NRNH2OHHHNH2OHNH3N2H4HAON2H4N24NR[5]ANAMMOX404598.4%73.3%99.7%86.9%DONRHHHAO4699.2%61NH4+-NNO2-N0.1280.140kg/(m3•d)0.2550.280kg/(m3•d)6175NH4+-NNO2-N82.6%80.8%TN73.8%1000mg/L100mg/L[15]220mg/L0.140kg/(m3•d)ANAMMOXNH4+-NNO2-N0.1250.138kg/(m3•d)63mg/L75mg/L70.3%68.7%58.7%1000.045kg/(m3•d)0.059kg/(m3•d)NH4+-NNO2-NTN1099.7%999.6%ANAMMOXANAMMOX85%6175889975.4%60.1%10UASB-ANAMMOXANAMMOX2.2pHANAMMOXpH9UASB-ANAMMOX·1675·NH4+NO2ANAMMOXCO2H+pH[12]3pHpHpHpHpH104pHANAMMOX9.08.58.07.5020406080100120t/dpH3pH2.3Strous[6]ANAMMOX1NH4+1.32NO20.066HCO30.13H+1.02N20.26NO30.066CH2O0.05N0.152.03H2O1ANAMMOXNH4+-NNO2-NNO3-N11.320.26NH4+-N0.26NO3-NNO3-NANAMMOX40.260.26ANAMMOX0.18[2]5NH4+3NO34N22H+9H2O2G0=297kJ/mol2604020020406080100120t/d/1030500.000.200.400.600.801.00/mg·L143pH7.98.20.128kg/(m3•d)0.140kg/(m3•d)84.2%82.7%75.6%7532434h32UASB-ANAMMOXANAMMOX5175.6%41.8%27.9%ANAMMOX4ANAMMOX1362.6%60%43ANAMMOXANAMMOXANAMMOXANAMMOX200928·1676·0.200.160.120.080.040.002030406080657075100t/d/kg·m3·d1TN5070/%TN8085909553pHpHANAMMOXH+430.180.27360.15100.29413NH4+-NNO2-N99.8%NH4+-NNO2-NNH4+-NNO2-NNH4+-NNO2-N92%95%TN85%NH4+-NNO2-NTN0.128kg/(m3·d)0.140kg/(m3·d)0.268kg/(m3·d)TN75.6%TN60%85%UASB-ANAMMOX2DOANAMMOXNRHHHAO3ANAMMOX4ANAMMOXANAMMOX[1]StrousM.Keyphysiologyofanaerobicammoniumoxidation[J].AppliedEnvironmentalMicrobiology199965(7)3248-3250.[2]Mosquera-CorralAGonzalezF.PartialnitrificationinaSHARONreactorinthepresenceofsaltsandorganiccarboncompounds[J].ProcessBiochemistry200540(4)3109-3118.[3]ChamchoiNNitisoravutSSchmidtJE.InactivationofANAMMOXcommunitiesunderconcurrentoperationofanaerobicammoniumoxidation(ANAMMOX)anddenitrification[J].BioresourceTechnology2008993331-3336.[4]ZhouShaoqiYaoJunqin.StartupanaerobicammoniumoxidationANAMMOXprocessinUASBbiofilmreactor[J].JournalofShanxiUniversityofScience&Technology200624(4)50-54.[5]JettenSMMWagnerMFuerstJetal.Microbiologyandapplicationoftheanaerobicammoniumoxidation(anammox)process[J].CurrentOpinioninBiotechnology200112283-288.[6]StrousMHeijnenJJKuenenJGetal.Thesequencingbatchreactorasapowerfultoolforthestudyofslowlygrowinganaerobicammoniumoxidizingmicroorganisms[J].Appl.Microbiol.Biotechnol.199850589-596.[7].[J].200524(6)629-631.[8].[J].200839(4)6-931.[9].16SrRNA[J].200829(2)469-473.[10].UASB[J].20082(1)11-15.[11].UASB-[J].200758(11)2798-2804.[12].[J].200634(5)1-4.[13].[M].2003.[14].[M].4.2002.[15].[M].2004.