稻草秸秆厌氧发酵产沼气研究英文

StudyonBiogasProductionUsingAnaerobicFermentationofRiceStrawLIUXiu-juan1LIHui1*XUEJin-hong2NIUXiao1WEIPing11．CollegeofBiotechnologyandPharmaceuticalEngineeringNanjingUniversityofTechnologyNanjing2118162．CollegeofPharmaceuti-calSciencesNanjingUniversityofTechnologyNanjing211816AbstractObjectiveTheaimwastoimprovethebiogasproductionrateandimprovetheuseratioofricestrawstothefurthest．MethodSingle-factormethodwasadoptedtoanalyzetheinfluenceofinoculatedamounttotalsolidcontentandcarbon-nitrogenratioonbiogasproduc-tioninbiogasstationofNanjingUniversityofTechnology．ResultItwasfoundthatthemostoptimumconditionwasinoculatedamountof34%totalsolidcontentof6%andcarbon-nitrogenratioof25∶1．Accordingtotheexperimentalverificationthetotalbiogasyieldinexperimen-talgroupincreasedby27．6%overthecontrolgroup．ConclusionTheresearchprovidedthereferenceofthefurtherindustrializationproductionofbiogasusingstraw．KeywordsRicestrawBiogasConditionoptimizationBiogasyieldReceivedSeptember92011AcceptedOctober232011SuppotedbytheNationalNaturalScienceFoundationofChina21046004theNationalKeyTechnologyR＆DProgram2011BAD15B02theNationalProgramofKeyBasicResearchProject2009CB724700theProvincialKeyTechnologyR＆DProgramBE20．*[e-mail]agetogetherwithagriculturalwastespollutionbecomemoreandmoreseriousthereforeproductionofbiogasbyanaerobicfermentationusingcropstrawandlivestockmanurehavebecomeafocus1．Andbyusingricestrawasrawmaterialtoconductbiogasproductionisoneoftheeffectivewaystosolvetheincreasinglyseriousenergyandenvironmentproblems2．Inactualproductioninordertohavehighbiogasproductionrateandrawmaterialusingratiothekeyfactorsinfluencingtheproductionofgasneedtobeexploredandanalyzed3．Atpresenttherearemanywaystoimprovethebiogasproductionsuchaschangingthematerialratio4addingex-ogenousaddictivesbrewer'sgrainspeptoneaminoacidmicroelement5etc．Thecropstrawswereselectedastheexperimentalmaterialofthisstudy．BasedontheproductionstatusinbiogasstationofNanjingUniversityofTechnologycombinedwiththeresearchesontheotherstrawsfermenta-tionproductionofbiogastechnology6－9thisstudyoptimizedthefollowingfactorsinoculatedamounttotalsolidcontentandcarbon-nitrogenratiowhichinfluencedtheproductionofbiogas．MaterialsandMethodsFeedstockandinoculumsThericestrawsweretakenfromLujiofSiyangCountyJiangsuProvinceair-driedandthensmashedinto3－5cmbygrindercompostedaftersoakedbyhumanexcrements．The7－dcompostedstrawswithappropriatefermentationinthemiddleweretakentostoreat4℃．TheinoculumsweretakenfromthebiogasfermenterofInnovationCenterMethaneGasStationofNanjingUniversityofTechnology．ThephysicalandchemicalcharacteristicsoffeedstockandinoculumswereshowninTable1．Table1Physicalandchemicalcharacteristicsoffeedstockandinoc-ulumsItemTotalsolid%C/NLignin%Cellulose%Hemice-llulose∥%Strawstalk29．530∶1112．6238．7519．99Inoculums418∶1－－－－meansnotdetected．ExperimentalapparatusesTheexperimentalapparatuseswereself-madeusingthe1000mlbottleasfermentationbottlefermentedinthe37℃waterbaththe5000mlwaterbottlewasservedasgascol-lectingbottleandthegasoutputwasmeasuredbydrainingwatergatheringofgaslaw．Single-factorexperimentMono-factormethodwasadoptedtoanalyzetheinocula-tedamountthepercentageoftheselectedfermentationbio-gasliquidinthetotalvolumeofthefermentationrawmateri-alstotalsolidcontentandcarbon-nitrogenratiowhichhaveimportantinfluenceonthebiogasfermentation．Theeffectofinoculatedamountonthebiogasproductionwasfirststudiedbyrespectivelyinoculating18%26%34%42%50%amountandaddingthecompostedstrawsintoeachfermenta-tionbottletomakethetotalsolidcontentto6%addinginocu-lumsaccordingtothedesignfullymixingaddingdistilledwa-tertomakethevolumeofthefermentationliquidto600ml．TheinitialpHofthefermentationliquidwasadjustedtomain-tainataround7byHClandNaOH．Afterfermentedfor15continuousdaysthedailybiogasyieldandtotalyieldwerecountedandanalyzed．Thenundertheconditionof34%in-oculatedamount25∶1nitrogen-carbonrationandpH7theeffectofdifferenttotalsolidcontent3%4%5%6%7%onbiogasproductionwasinspected．Finallytheopti-mizedproportionwastestedinthe25Lfermenterandchan-gesofthetotalsolidcontentcellulosecontentandtotalbio-gasyieldbeforeandafterbiogasfermentationweremeasuredatthesametime．MeasurementitemsandmethodsThetotalsolidcontentwasmeasuredbythemethodusinginroutineanalysisofbiogasfermentation10thetotalcarbonamountwasmeasuredbypotassiumdichromateoxi-AgriculturalScience＆Technology201112121761－1764Copyright2011InformationInstituteofHAAS．Allrightsreserved．AgriculturalBasicScienceandTechnologydation-outsideheatingmethodthetotalnitrogenwasmeas-uredbyKjeldahiazotometer11thecontentsoflignincellu-losehemicelluloseweremeasuredbytherawmaterialcom-ponentanalysismethodusedbyAmericarenewableenergysourceslaboratoryNREL12．ResultsandAnalysisEffectofinoculatedamountonbiogasproductionTheamountoftheinoculumsaffectedtheoperationeffectandstabilityofthebiogasfermentation．Iftheinoculatedamountwastoosmallthenumberofmethanogenwouldbenotenoughandresultedintheslowstartingoffermentationandreductionofmethane-generatedspeediftheinoculated