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20420017JournalofWuxiUniversityofLightIndustryVol.20No.4Jul.2001:1009-038X(2001)04-0351-05:2000-10-13;:2001-04-13.:(96-C03-01-01).:(1946-),,,.,,(,214063):,IMO-50.IMO-50,,IMO-90.:;;;:TS245.4:ATheAdvancedSeparationTechnologyofIsomaltooligosacchrideBAOYuan-xing,SUNWe-irong,YIANGWe-iya(JiangsuInstituteofMicrobiology,Wuxi214036,China)Abstract:Thispaperexpoundedabouthowtousetheseparationtechnologyofnanofiltration,chro-matography,andyeastfermentationtoproducehighpurityisomaltooligosacchrideIMO-90.Keywords:nanofiltration;chromatography;yeastfermentation;Isomaltooligosacchride1[1~6].,,.:180,342,504,666.180,.1.1,,,1.,.1Tab.1Hold-backratescomparsionamongthreeexperimentalmembranes/%/%8099.0,IMO-909399.5,,IMO-908090.0,,1.22.540,,,.40kg/d.1.3:1.5106~1.8106Pa;:15~20L/min;:40~45;:.1.41),;,.HPLC2.2Tab.2HPLCanalysisofhold-backfluid%G1G2IG2G3PIG3Gn(n4)IG2+P+IG3IMOIMO56.355.4625.172.3312.0814.5334.0851.3885.8692.5473.21103.516.3025.172.2714.0813.6035.0752.8587.9288.3767.86152.916.5625.102.1215.3812.6935.2153.1788.3879.5660.71202.366.1625.472.4112.8314.9435.8353.2489.0775.3157.14251.436.1825.481.215.5913.9036.1254.9791.0973.5753.30301.306.5125.052.1715.1913.3436.4453.3890.0274.2255.712),,1.1Fig.1Relationshipbetweenisomaltooligosacchridepu-rityconstituentrecyclerateandpurificationtimes85%(900,85%),,,90%.,.53.2.[7~8].2.16,.,.,G1IMO,.V12,S2S33.3Tab.3Analysisofhighpurityisomlatoligosacchrideofnanofiltration%G1G2IG2G3PIG3Gn(n4)IG2+P+IG3IMO11.436.1825.581.2015.5913.9036.1255.0791.1921.445.2328.831.6112.3412.6637.8953.8391.7231.265.2027.952.4610.4713.0439.6251.4691.0841.075.4128.612.2111.5911.9039.2152.191.3151.094.1929.871.2412.8511.9938.7754.7193.48352202V1Fig.2ChromatographyelutioncurveofresinV1sepa-ratingsyrup3S2S3Fig.3ChromatographyelutioncurveofresinS2,S3sep-aratingsyrup,,.46IMOTab.4IMOamountandrecyclerateofsixresinswashfluidIMO/%/%S164.155.1S289.745.0S384.272.4P180.742.2P284.211.9V185.129.3S1S2S3;P1P2V1.,S2S3V1.2.2,.P1IMO80.7%,95.8%,42.2%47.8%,.2.32m4m,.,;,;;;,.2.4,CSEP,IMO-90.5.5CSEPTab.5TechnicalparametersofCSEP95cm6.5610-3m320(D21mm1000mm)60115mL/min8mL/minIMO43mL/min51mL/min50%3%HPLCCSEP19,IMO,,4.4Fig.4Continuouschromatographycurve3534:,1~10,HPLC,IMO85%.2.5,CSEP,8h,.,6.6Tab.6Analysisofhighpurityisomatooligosacchrideofchromatogramseparation%IMO12.147.6919.542.1913.4311.0943.6487.7021.958.9917.661.3915.2910.6143.0886.6432.199.3321.001.6715.0211.2338.8486.0942.6310.3123.571.6113.8713.3634.2885.0853.306.2922.271.6910.8515.3838.2286.7233.1,-1,6,.6,,pH[9,10],.3.2IMO-90IMO-90,3,.3.33.3.112.0~2.5,,30~32,pH4.5~5.0,1.0%~1.5%,8~10h,8107/mL,.3.3.2,,30~32,pH4.5~5.0,,,40h.().3.3.3,.3.3.412~3.3.3.512m3/,(215m3),,.,,,.,98%~99%,53%,7.41)91%~93%,IG2+P+IG351%~55%.,,.,90%,,,,,,,,.,.2),,354207Tab.7Analysisofhighpurityisomaltooligosacchrideofyeastfermentation%IG2+P+IG3IMO129.050.8611.4614.1644.4754.6799.14231.371.1211.0313.5942.8955.9998.88329.461.2810.8514.4044.0154.7198.7240.0863.4430.721.3610.0313.9940.3754.7495.1150.564.1329.251.4510.6513.3740.5953.2793.86,.3),,94%~99%.,,.,.4)IMO-90,,.,.5)IMO-50IMO-90,.:[1].[M].:,1999.[2].[M].:,1994.[3].[M].:,1999.[4].[M].:,1999.[5].[M].:,1996.[6],,.[J].,1999,18:159~161.[7],.[M].:,1994.[8],.[J].,1999(4):17~20.[9].[M].:,1982.[10],.[J].,1995(9):21~22.[11].[M].:,2000.[12],,.[J].,1999(3):8~9.(:)3554: