低浓度乙酸盐诱导下厌氧氨氧化与异养反硝化高效耦合脱氮张美雪

1011Vol．10No．11201611ChineseJournalofEnvironmentalEngineeringNov．2016111*122221．1001242．100012。。NH+4-N30～40mg·L－1、NO－2-N45～55mg·L－1、CH3COONa60～80mg·L－1NH+4-N、NO－2-NTN93．84%、94．62%86．46%。、。12．46mg·gMLSS·h－1。ANAMMOXAOBNOB。1．893．59mg·gMLSS·h－1。。X703A1673-9108201611-6127-06DOI10．12030/j．cjee．201506020NitrogenremovalperformanceofanammoxcouplingheterotrophicdenitrificationinlowconcentrationofacetateZHANGMeixue1LIYun1LIJun1*ZHENGZhaoming1XIONGXiangyang2CHENGang2YAOYuan2LIQiang21．TheCollegeofArchitectureandCivilEngineeringBeijingUniversityofTechnologyBeijing1000124China2．ChinaUrbanConstructionDesignandＲesearchInstituteCo．Ltd．Beijing100012ChinaAbstractWeinvestigatedthenitrogenremovalperformanceofanaerobicammonium-oxidizinganammoxgranulesinareactorundercontinuouscultivationatlowacetateconcentration．Asacomparisonweappliedbatchteststomeasurethenitrogentransformationandremovalpathwaysofgranulesobtainedfromthecouplingreactor．Thereactorexhibitedexcellentanammoxanddenitrifyingperformancesatlowacetateconcentration．DuringsteadyphaseremovalratesofNH+4-NNO－2-NandTNwere93．84%94．62%and86．46%respectively．Batchtestsindicatedthatthecouplinganammoxgranulesexhibitedgoodanammoxnitrificationanddenitrificationactiv-ities．Thetotalnitrogenremovalrateofcouplinggranuleswas12．46mg·gMLSS·h－1．Nitrifyingbacteriainthesystemwerecapableofconsumingthedissolvedoxygenthuslesseningtheinhibitoryeffectofoxygenonanam-moxbacteria．Asaresultactivityofammonia-oxidizingbacteriaAOBwashigherthanthatofnitrite-oxidizingbac-teriaNOB．Denitrificationratesfornitriteandnitratewere1．89and3．59mg·gMLSS·h－1respectively．Whennitriteandnitratewerepresentsimultaneouslydenitrifyingbacteriareducednitratetonitritefirst．KeywordsacetateANAMMOXheterotrophicdenitrificationnitrogenremoval2014ZX07201-0118122005Y07H130742015－06－032015－07－071989—。[e-mail]*[e-mail]10anaerobicammoniumoxidationAN-AMMOX、、。12。ANAMMOXNO－3-NCO2ANAMMOX3。ANAMMOX4ANAMMOX。。。5-67-8CO2ANAMMOX。ANAMMOX。11．1up-flowanaerobicsludgeblanketUASB110L1/310cm。25℃HＲT1．2h。1．250LUASBANAMMOX213Candidatus-BrocadiafulgidaJX852965-JX852969。1．3NH4Cl、1UASB-Fig．1SchematicdiagramofUASB-biofilmreactorNaNO2、NaHCO3、KH2PO4、MgSO4·7H2O、CaCl2CH3COONa。Ⅰmg·L－1EDTA5000FeSO45000Ⅱmg·L－1EDTA15000ZnSO4·7H2O430CoCl2·6H2O240MnCl2·4H2O990CuSO4·5H2O250Na2MoO4·2H2O220NiCl2·6H2O190Na2SeO4·10H2O210H3BO314。Ⅰ、Ⅱ1mL·L－1。1．41ANAMMOX25～30℃1．2hpH7．5～8．0。1Table1Experimentalplanandoperationalconditionsofcontinuousflow/dNH+4-N/mg·L－1NO－2-N/mg·L－1NO－3-N/mg·L－1CH3COONa/mg·L－1A1～1730～4035～451～530～50B18～5530～4035～451～860～80C56～9530～4045～552～1060～80500mL25±1℃4hpH7．5～7．899．999%30min0．25L·min－1200r·min－1。8216111．5NH+4-NNO－2-NN-1--NO－3-NMLSS、MLVSSpH、WTW/Multi3420。22．1ANAMMOX。50～60mg·L－140～50mg·L－1。NH+4-N、NO－2-NNO－3-N1∶1．30∶0．259。NH+4-N、NO－2-NTN67．16%、64．60%56．97%。2。1～17d30mg·L－1。NH+4-N、NO－2-NTN57．11%、62．69%51．07%NO－2-NNH+4-NΔNO－2-N/ΔNH+4-N1．25NO－3-NNH+4-NΔNO－3-N/ΔNH+4-N0．219ΔNO－2-N/ΔNH+4-NΔNO－3-N/ΔNH+4-N。60～80mg·L－110dOH－pH27dpH7．5～8．010d54NH+4-N、NO－2-NTN83．45%、97．96%83．17%ΔNO－2-N/ΔNH+4-N1．23ΔNO－3-N/ΔNH+4-N0．11。55NO－2-N/NH+4-N1．29NH+4-N、NO－2-NTN93．84%、94．62%86．46%ΔNO－2-N/ΔNH+4-N1．31ΔNO－3-N/ΔNH+4-N0．05ΔNO－2-N/ΔNH+4-NΔNO－3-N/ΔNH+4-N2NH+4-N、NO－2-N、NO－3-NTNFig．2ＲemovalcharacteristicofNH+4-NNO－2-NNO－3-NandTNinsystem9216103Fig．3StoichiometryofANAMMOXduringoperationofANAMMOX-UASB。2．2。ANAMMOX、。2．2．14Fig．4Anammoxactivityofgranularsludge4a4b70mg·L－1。。5．417．50mg·gMLSS·h－11．56mg·gMLSS·h－111．34mg·gMLSS·h－1。70mg·L－15．567．78mg·gMLSS·h－1－0．31mg·gMLSS·h－113．65mg·gMLSS·h－1。10411-12。60～200mg·L－113222mg·L－127．05%。ANAMMOX7CO2KAＲTAL14ANA-MMOXAnammoxoglobuspropi-onicusBrocadiafulgidaＲATTＲAY1513CCH133COOHANAMMOXBrocadiafulgidaA。。。。。2．2．20316114～6mg·L－1。ammoniaoxidationbacteriaAOBnitriteoxidationbacteriaNOB。。5a5bAOBNOBAOB2．11mg·gMLSS·h－10．980．14mg·gMLSS·h－1NOB0．240．26mg·gMLSS·h－1。AOBNOB。ANAMMOXANAMMOX16-170．5%ANAMMOX18。AOBNOBAOBNOB19-20AOBNOBAOBANAMMOXNO－2-N。5Fig．5Nitrificationactivityofgranularsludge2．2．3。。、3。6a1．89mg·gMLSS·h－13．59mg·gMLSS·h－1。6b3h412。6Fig．6Denitrificationactivityofgranularsludge31131610。NH+4-N、NO－2-NTN93．84%、94．62%86．46%。2、。12．46mg·gMLSS·h－1。ANAMMOXAOBNOB。31．893．59mg·gMLSS·h－1。。1VANDEGＲAAFA．A．DEBＲUIJNP．ＲOBEＲTSONL．A．etal．Autotrophicgrowthofanaerobicammonium-oxidizingmicro-organismsinafluidizedbedreactor．Micro-biology199614282187-21962KUMAＲM．LINJ．G．Co-existenceofanammoxanddenitrificationforsimultaneousnitrogenandcarbonremovalStrategiesandissues．JournalofHazardousMaterials20101781/2/31-93．．20063451-4ZHOUShaoqi．Stoichiometricanalysisofcombinedreactionofanaerobicammoniaoxidationwithdenitrification．JournalofSouthChinaUniversityofTechnologyNaturalScienceEdition20063451-4inChinese4MOLINUEVOB．GAＲCIAM．C．KAＲAKASHEVD．etal．Anammoxforammoniaremovalfrompigmanureef-fluentsEffectoforganicmattercontentonprocessperform-ance．BioresourceTechnology200910072171-21755．．200929102041-2047LIUJinlingZHONGYumingXIEZhiruetal．Metabo-lismofanaerobicanmoniumoxidizinganammoxbacteriawithorganiccarbonaddition．ActaScientiaeCircumstanti-ae200929102041-2047inChinese6．．20133312216