低强度超声波改善污泥活性

1,2,2,2(1.,100083;2.,100875):,(OxygenUptakeRate,OUR),0112W/cm2040min.013W/cm2,10min,,.,.,013W/cm2,10min048h,8h,2,24h.,013W/cm28h10min,..:;;;;;;:X70311:A:025023301(2005)0420124205:2004207224;:2004209210:(2002DFBA0009):(1964),,,,,.E2mail:[e-mail]tyofActivatedSludgebyLowIntensityUltrasoundLIUHong1,YANYi2xin2,WANGWen2yan2,YUYong2yong2(1.DepatmentofEnvironmentalEngineering,BeijingUniversityofAeronauticsandAstronautics,Beijing100083,China;2.SchoolofEnvironment,BeijingNormalUniversity,Beijing100875,China)Abstract:Aerobicactivatedsludgefromdomesticwastewatertreatmentplantwasusedasanexperimentalmaterial.OxygenUptakeRate(OUR)wasdetectedastheindextoindicatethechangesofsludgeactivityinducedbyultrasound(US)at35kHzin040minwiththeUSintensitiesof0112W/cm2.ThebestresultswereachievedataUSintensityof013W/cm2andirradiationtimeof10min.ImproperUSintensityandirradiationtimeleadtoadecreaseofsludgeactivity.Therefore,thestimulatingeffectsofUSonthesludgeactivitycouldbeenhancedatanoptimizedUSintensityandirradiationtime.Inaddition,changesofsludgeactivities(within48hours)afterUSirradiationat013W/cm2in10minwereexamined.Theactivityofsludgetook8hoursafterirradiationtoreachpeaklevel,andtheactivitywasasdoubleasthatoftheinitiallevelafterirradiation.In24hoursafterirradiation,theen2hancementeffectsinducedbyultrasounddisappeared.Therefore,toimprovethetreatmentefficiencyofbiologicalreactor,ultrasoundwithintensityof013W/cm2couldbeemployedtoirradiatepartofthesludgeinthebiologicaltreatmentsystemfor10minutesevery8hours.Italsopresentedthehypotheticalexplanationofthemechanismofbiologicalactivityenhancementstimulatedbyultrasound.Keywords:lowintensityultrasound;activatedsludge;oxygenuptakerate(OUR);SVI;ultrasoundintensity;irradiationtime;treat2mentcycle20kHz,[1].,[2].10W/cm2[3].,,,[4],.;[5,6].,,,,,,[3].,,[7,8].Schlafer[8],COD100%,,,,.Lin[9],,2,26420057ENVIRONMENTALSCIENCEVol.26,No.4Jul.,2005©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.中国城镇水网[1012].,(OxygenUptakeRate,OUR)(SludgeVolumeIndex,SVI),,;,,.11.11.1Fig.1SchematicdiagramofultrasoundirradiationsystemDL260D:,35kHz,080W.,,,.1.2,250mL,70mL,9962mg/L,4,.1.2.120min,OURSVI,.,,OUR,.2.1.2.2,2L9962mg/L21L,.16L,8L(1)SBR.8h,,6h1h,1h.,OUR,OUR.1/mgL-1Table1Characteristicsofsyntheticdomesticwastewater/mgL-1KH2PO4CaCl2MgCl2CuSO430022.57.320.81.00.0021.3(MixLiquorSuspendedSolids,MLSS)OURSVI.MLSSSVI[13].OUR,2.3,,(1),,2526,DO,DO2t.MLSS,:OUR=(DO1-DO2)/MLSS(t2-t1),OUR:,mg/(gmin);DO:,mg/L;t:,min;MLSS:,g/L.CS23.,5214©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.中国城镇水网(Tektronix,USA).55/12FT:IncorporatedYellowSpring,Ohio,USA8122::250mL,350mL2OURFig.2ExperimentalsetupformeasuringOUR22.12.1.1OUR3.,,,,013W/cm2,.,,015W/cm2,.3Fig.3Effectofultrasoundintensityonsludgeactivity,,.,,,,.,(013W/cm2),,,015W/cm2,,,,,.2.1.2SVI4,,SVI,.015W/cm2,SVI.013W/cm2SVI.4Fig.4Effectofultrasoundintensityonsettleabilityofsludge,,,,,,[14].,,,,.,,,,.2.2,013W/cm2,040min,,5.5,,,10min.,,.,62126©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.中国城镇水网[5],,.5Fig.5Effectofirradiationtimeonsludgeactivity2.3,,.013W/cm210minOUR6,OUR.6Fig.6Changesofactivitiesofsludgeafterultrasoundirradiation6,,8h,1.,,.,,16h,24h.,,,,,,OUR;,,,,,,,,8h,,,,,.,,16h.,,.,,,.8h,.2.4013W/cm2,5mm,(7).7Fig.7Distributionofultrasoundfieldinsideofthereactor7,,,.,.,7214©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.中国城镇水网(1),,013W/cm2,.(2),.,,,,,,013W/cm2.(3),10min.(4),8h,1,24h.013W/cm28h10min,.:,.:[1].[M].:,1990.17.[2],,,.[J].,1994,21(6):500503.[3]PittWillamG,RossSAaron.UltrasoundIncreasestheRateofBacterialCellGrowth[J].Biotechnol.Prog.,2003,19(3):10381044.[4]LiuYiyao,TakatsukiHideyo,YoshikoshiAkio,WangBochu,SakanishiAkio.EffectsofultrasoundonthegrowthandvacuolarH+2ATPaseactivityofaloearborescenscalluscells[J].ColloidsandsurfacesB:Biointerfaces,2003,32:105116.[5]SchlaferO,SieversM,KlotzbucherH,OnyecheTI.Improve2mentofbiologicalactivitybylowenergyultrasoundassistedbioreactor[J].Ultrasonics,2000,38(128):711716.[6]WuJianyong,LinLidong.Ultrasound2inducedstresspresponsesofPanaxginsengcells:enzymaticbrowningandphenolicspro2duction[J].Biotechnol.Prog.,2002,18:862866.[7]WoodBE,AldrichHC,IngramLO.Ultrasoundstimulatesethanolproductionduringthesimultaneousscarificationandfer2mentationofmixedwasteofficepaper[J].Biotechnol.Prog.,1997,13:232237.[8]SchlaferO,OnyecheT,BormannH,SchrroderC,SieversM.Ultrasoundstimulationofmicro2organismsforenhancedbiodegradation[J].Ultrasonics,2002,40(128):2529.[9]LinLidong,WuJianyong.Enhancementofshikoninproductioninsingle2andtwo2phasesuspensionculturesofLithospermumerythrorhizoncellsusinglow2energyultrasound[J].Biotechnolo2gyandBioengineering,2002,78(1):8188.[10]WangBC,YoshikoshiA,SakanishiA.Carrotcellgrowthre2sponseinastimulatedultrasonicenvironment[J].ColloidsandSurface.B:Biointerfaces,1998,12:8995.[11]LiuYiyao,YoshikoshiAkio,WangBochu,Sakanish