低强度超声波强化生物降解硝基苯的研究袁源

*116024R．mucilaginosa、S．albidoflavusM．luteus。180W35kHz15min。96h11.3%、7.9%1.3%。151716h。Z1Z252h5hZ3。Andrews。Z1＞Z2＞Z3。STUDYONDEGRADATIONOFNITROBENZENEENHANCEDBYLOWINTENSITYULTRASOUNDYuanYuanJinRuofeiZhouJitiKeyLaboratoryofIndustrialEcologyandEnvironmentalEngineeringMOESchoolofEnvironmentalScienceandTechnologyDalianUniversityofTechnologyDalian116024ChinaAbstractInthispaperthelow-intensityultrasoundLIUgroupandthecontrolgroupwereemployedtoinvestigatethemicrobialdegradationofNBwithRhodotorulamucilaginosaZ1StreptomgcesalbidoflavusZ2andMicrococcusluteusZ3underdifferentconditions．Theoperationconditionswereoptimizedas35kHz180Wand15minforirradiation．ComparedtocontrolgrouptheNBremovalsinLIUgroupwereincreasedby11.3%7.9%and1.3%in96handtheinitialultrasoundeffectswereobservedat15h17hand16hincontinuousexperimentsrespectively．ForZ1andZ2theeffectdurationwas52hand5h．NoobviousdurationonZ3indicatesthatLIUcannotimproveallthemicrobialdegradationofNB．ThedegradationcurvesfittheAndrewsmodelwellandthekineticparameterswereimprovedbyultrasound．Thelowintensityultrasoundhasdifferenteffectonthethreestrainsinkinetics．Keywordsnitrobenzenelowintensityultrasoundenhacement*508082029200902538。0、、、1。、《》2。3。。4。5。611-12。6。7。、。99201112296DOI:10.13205/j.hjgc.2011.06.02111.1R．mucilaginosaZ1GenBankDQ778627S．albidoflavusZ2GenBankDQ855477M．luteusZ3GenBankDQ8554768。Z1Z2Z3。1.20.5g/L1.0g/L7.0g/LNa2HPO4·12H2O1.0g/LKH2PO40.01g/LCaCl2·2H2O0.02g/LMgSO4·7H2O0.002g/LpH=7.0。1.3480mg/L7.0g/LNa2HPO4·12H2O1.0g/LKH2PO40.01g/LCaCl2·2H2O0.02g/LMgSO4·7H2O0.002g/LpH=7.0。22.1－80℃30℃、pH=7.0、150r/minOD6600.6～0.7Na2HPO4-NaH2PO40.02mol/LpH=7.03OD6600.7～0.8。2.2100120140160180200W、253035℃51015min。96h。2.396h。2.4。。2.5qh－1。2.69-10λ=268nmy=0.0157x+0.0141R2=0.999。=A0－A/A0×100%A0A。33.11。1。1/kHz/W/℃/minZ1251802515Z225160255Z3251802515113。196h84.2%、76.4%65.8%111.3%、7.9%1.3%。001201112296Z1＞Z2＞Z3。a—Z1b—Z2c—Z3296h3.2。14。2aZ115h15h96h11.3%15h。2b2cZ2Z317h16h。Z1。3.3。3aZ120h。72h。52h。3bZ25h。3cZ3。。a—Z1b—Z2c—Z33144h3.4。Andrews101201112296。Andrewsq=qmaxSKs+S+S2Kiqh－1Smg/Lqmaxh－1Ks、Kimg/L。4。a—Z1b—Z2c—Z34Andrews2。2qmaxKsKi。Z3。。2qmaxKsKiqmaxKsKiZ15.72476.3434.677.21502.1947.23Z25.13481.2146.735.86500.3259.25Z34.25424.6137.244.27432.1239.1741。Z1、Z2Z3180160180W15515min25℃。2151716h。Z1Z252h5hZ3。。3Andrews。qmax、KsKiZ3。4。1SpainJC．BiodegradationofnitroaromaticcompoundsJ．AnnualReviewofPharmacologyandToxicology1995494523-555．2TangPZhouJWangTetal．AdvanceinnitrobenzenewastewatertreatmentJ．IndustrialWaterTreatment200323316-19．3．J．200284427-429．4．M．1994．5．J．200626740-43．6．J．200284427-429．57201201112296。ASM2D、。1．UNITANKJ．199915721-23.2．J．200321425-28.3．UNITANKJ．1998241116-19.4．UNITANKJ．20022831-4.5．UNITANKJ．200521175-78.6．UNITANKJ．200521366-69.7．UNITANKJ．200824292-94.8．CAST、UNITANK、J．201026245-48.9．．M．2002.10WGujerMHenzeMinoTetal．ActivatedsludgemodelNo．2DJ．WatSciTech1999391165-182.11ZhangFagenLiuJunxinSuiJun．SludgedynamicdistributionanditsimpactontheperformanceofUNITANKJ．JournalofEnvironmentalScience2007192141-147.12．ASM2DUNITANKJ．200733518-21．13．UNITANKJ．20072611-4.14．UNITANKJ．20077142-45.15．UNITANKJ．20062810731-733.16．UNITANKJ．200622986-89.17．UNITANKJ．200622293-95.18．UNITANKJ．200624635-36.19．J．．2006329[e-mail]2011－04－21櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅1027．Comamonassp．CNB1J．20044448-12．8．J．2008482173-177．9．J．200020137-38．10．M．4．2002．11LessnerDJJohonsonGRParalesREetal．MolecularcharacterizationandsubstratespecificityofnitrobenzenedioxygenasefromComamonassp．strainJS765J．AppliedEnvironmentalMicrobiology2002682634-641．12ParkHSLimSJChangYKetal．DegradationofchloronitrobenzenesbyacoclutureofPseudomo-nasputidaandaRhodococcusspJ．AppliedEnvironmentalMicrobiology19996531083-1091．13．J[e-mail]2011－06－2175201112296