低溶解氧环境下全程硝化活性污泥的特性高瑶远

2017,37(5)1769~1774ChinaEnvironmentalScience,*,,,(,,100124),,SBR,,0.3~0.5mg/L,.,25,pH8.5;pH7.5,20,0.5~4mg/L,0.30.6mgN/(mgMLSSd).,Nitrospira,33%;Nitrosomonas,7%.pHX703.5A10006923(2017)05176906Thecharacteristicofactivatedsludgeinnitrifyinglow-DOreactor.GAOYao-yuan,PENGYong-zhen*,BAOPeng,GUOSi-yu,WANGShu-ying(NationalEngineeringLaboratoryforAdvancedMunicipalWastewaterTreatmentandReuseTechnology,EngineeringResearchCenterofBeijing,BeijingUniversityofTechnology,Beijing100124,China).ChinaEnvironmentalScience,2017,37(5)1769~1774AbstractThecharacteristicsandmicrobialcommunityofnitrifyingbacteriacultivatedatthelowDOconcentrationof0.3~0.5mg/Linasequencingbatchreactor(SBR)wasinvestigatedinthisstudy.Thebacterialabundanceanddiversitywereanalysedwithdifferentinfluencingfactorsaftersuccessfulcultivationoftheactivesludge.Resultsshowedthat,theoptimumtemperatureforlow-DOnitrifyingbacteriawas25,andtheoptimumpHwas8.5.UndertheconditionofpH7.5at20,specificammoniaoxidaterateandspecificnitriteoxidateratewere0.3and0.6mgN/(mgMLSSd)withDOof0.5~4mg/L,respectively.HighthroughputsequencinganalysisrevealedthatNitrospiragenuswasthedominantnitriteoxidizingbacteria(NOB)accountingfor33%ofwholecommunity,andNitrosomonaswasthemajorammoniumoxidizingbacteria(AOB)accountingfor7%inthelow-DOnitrificationreactor.KeywordslowdissolvedoxygennitrifyingbacteriatemperaturepH[13].[45],50%~80%[68].,[9],,[10][1112].[1314],.,5%,.,.,,,pH,20160927(51478013);*,,[e-mail]177037.11.1SBR,10L(8L).(25±1),100r/min,,.pH7.5~8.0.0.3~0.5mg/L.,,.417min,7min,360min,30min,20min;600min,.1.2,3~7mg/L,NH4Cl,50~70mg/L,80~125mg/L.,:0.8gNaHCO3,0.04gK2HPO40.02gMgSO4·7H2O0.5mL.[15]:1.25gEDTA,0.55gZnSO4·7H2O,0.4gCoCl2·6H2O,1.275gMnCl2·4H2O,0.4gCuSO4·4H2O,0.05gNa2MoO4·2H2O,1.375gCaCl2·2H2O,1.25gFeCl3·6H2O.SBR,.1.3pH,:500mL5,300r/min,500mL,50mg/L20mg/L500mL.3:(1)1015202530,0.5mg/L,pH7.5;(2)pH77.588.59,0.5mg/L,20;(3)0.51234mg/L,pH7.5,20.5,,,(SAOR,mgN/(mgMLSS·d))(SNOR,mgN/(mgMLSS·d)).404[NHN][NHN]SAOR=MLSSTT330[NON][NON]SNOR=MLSSTT:T,d;[NH4+N]0,mg/L;[NH4+N]TT,mg/L;MLSS,mg;[NO3N]0,mg/L;[NO3N]TTmg/L.1.4:;:N1;:;.pHDOWTWMulti340i(WTW,);(MLSS):;(SV):30min.1.5DNA,150d.,FastDNASpinKitforSoil(QBIOgenInc.,Carlsba,CA,)DNADNA.16SrRNAV3V4[16],338F(ACTCCTACGGGAGGCAGCA)806R(GGACTACHVGGGTWTCTAAT).PCR20μL:4μL5×FastPfuBuffer2μLdNTPs0.4μL/0.4μLFastPfuPrimer10ngDNAddH2O20μL.:952min;25(9530s,5530s,7240s);7210min.DNAIlluminaMiSeq.22.11.,NH4+N60mg/L51771,360min,,NH4+NNO2N0.1~0.3mg/L.30d,30dNH4+N120mg/L,,,,40mg/L,600min,,0.1~0.3mg/L.NH4+N,,,HRT,,,,.204060801001201401600255075100125150175(mg/L)(d)NH4+-NNH4+-NNO2--NNO3--N1NH4+NNH4+NNO2NNO3NFig.1InfluentconcentrationofNH4+NandeffluentconcentrationofNH4+N,NO2N,andNO3NduringthelowDOdomesticatedperiod204060801001201401600600120018002400300036004200MLSSSV(d)MLSS(mg/L)1020304050SV(%)2Fig.2MLSSandSVofreactorduringthelowdissolvedoxygendomesticatedperiod,2,(MLSS)(SV),MLSSCOD,(,).80d,MLVSS1070mg/L,MLVSS/MLSS0.86,MLSSSV,80d,.2.2DO,(150d),DO.3,DO:NitrospiraNitrosomonasAnaerolineaceae_unculturedDokdonellaFerruginibacterFlexibacter.NitrosomonasAOB[17],NitrospiraNOB[13].,,DONitrosomonasNitrospira.[18]AOBNOBNitrosomonasNitrospira.0%20%40%60%80%100%NitrospiraDokdonellaFemuginibocterOthersAnoerolineoceoe_unculturedNitrosomonosFlexibacter3Fig.3Microbialcommunityofactivatedsludge,NOBNitrospira(33%)AOBNitrosomonas(7%),NOB177237(Nitrospira)DO.,Liu[13]Bao[19]DONitrospira[13,19].2.3DO2.3.120~30,35,,10[2021].10~30,4.10152025300.000.150.300.450.600.750.90[mgN/(mgMLSSd)]()4Fig.4Specificnitrifyingrateoflowdissolvedoxygendomesticatednitrifyingbacteriaunderdifferenttemperature410~25,,25,0.520.60mgN/(mgMLSS·d),30.10,,,Euiso[20],10;[22]AAOBAF15,.25~30,,[23],25~30[24],35.2.3.2pHpH,[25].1gNH4+N7.14g,,pH.pH3:1)pH;2)pH,;3)pH(FA)(FNA)[26].pH,pH5.77.588.590.000.150.300.450.600.750.90[mgN/(mgMLSSd)]pH5pHFig.5SpecificnitrifyingrateoflowdissolvedoxygendomesticatednitrifyingbacteriaunderdifferentpH5,pH,pH8.5,0.520.68mgN/(mgMLSS·d).pH7517738.5,0.21mgN/(mgMLSS·d)0.52mgN/(mgMLSS·d),148%;0.510.68,33%,AOBNOBpH.pHpHFA,pHFA:-324NH+HONH+OHpH4pHbw[NHN]1017(FA)1410KK:Kb;Kw.,FApH,.FA[27],FA,pH7.5~8.50.5~0.6mgN/(mgMLSS·d).[28],pH,,pH8.5,.0.512340.000.150.300.450.600.750.90[mgN/(mgMLSSd)]DO(mg/L)6Fig.6SpecificnitrifyingrateoflowdissolvedoxygendomesticatednitrifyingbacteriaunderdifferentDO2.3.3,DO2.0mg/L[14],DO,DO,,.DO6.6DO0.5~4mg/L,,0.30.6mgN/(mgMLSSd),[28][29]DO.160d,,DO.DODO,,.,DO,DO.,DO,,.33.10.3~0.5mg/L,,70d.3.225pH8.5;pH7.5,20,0.5~4mg/L,0.30.6mgN/(mgMLSSd).3.3,NOBNitrospira33%,AOBNitrosomonas7%,.[1],,.[J].,2005,24(4):3638.[2],.[J].,2001,20(1):2427.177437[3],,,.[J].,2004,22(2):2527.[4],,.[J].,2001,19(4):48.[5],,,.[J].,2007,24(2):231234.[6].[J].,1984,(6):1721.[7],,,.[J].,2009,35(s1):270274.[8],,,.[J].,2011,27(4):3336.[9]GeS,WangS,YangX,etal.Detectionofnitrifiersandevaluationofpartialnitrificationforwastewatertreatment:Areview.[J].Chemosphere,2015,140:8598.[10]PengY,ZhuG.Biologicalnitrogenremovalwithnitrificationanddenitrificationvianitritepathway.[J].AppliedMicrobiologyandBiotechnolo