低溶解氧污泥微膨胀前后污泥硝化活性的对比研究左金龙

第31卷第4期土木建筑与环境工程Vol.31No.42009年8月JournalofCivil,Architectural&EnvironmentalEngineeringAug.2009左金龙1,3,王淑莹2,彭赵旭1,姜安玺1,刘　洋4,彭永臻1,2,孙治荣2(1.哈尔滨工业大学市政环境工程学院,哈尔滨150090;2.北京工业大学环境与能源学院,北京100022;3.哈尔滨商业大学环境工程系,哈尔滨150076;4.中国水务控股有限公司(新加坡),北京100022):2009-03-15:(50778005);(863)(2006AA06Z319);(EDBS07/1-53974092);(LBH-Z08200):(1970-),,,,(E-mail)[e-mail]。(),,,,,(E-mail)[e-mail]。　:为了研究低溶解氧微膨胀前后污泥硝化活性的变化,采用SBR反应器,平均DO浓度为0.6mg/L～0.9mg/L,测定污泥微膨胀前后污泥氧消耗速率曲线。结果表明:发生污泥微膨胀后,活性污泥对COD的去除能力有较大的提高,而对氨氮去除能力却有一定的下降。污泥微膨胀前后的氧消耗速率曲线显示,微膨胀前活性污泥总活性为67.72mgO2/gVSS·h,其中硝化活性为43.12mgO2/gVSS·h,占其总活性的63.67%;而微膨胀后活性污泥总活性为90.49mgO2/gVSS·h,其中硝化活性为23.98mgO2/gVSS·h,占其总活性的26.51%。低DO成为微生物生长的限制性基质,污泥微膨胀的状态下,活性污泥中丝状菌成为优势菌种,而硝化细菌成为非优势菌种,污泥的总硝化活性降低。:低溶解氧;污泥微膨胀;硝化;硝化菌;丝状菌:X703.1　　:A　　:1674-4764(2009)04-0117-06ComparisonAnalysisofSludgeNitrificationActivityBeforeandAfterMicro-bulkingwithLowDissolvedOxygenZUOJin-long1,3,WANGShu-ying2,＊,PENGZhao-xu1,JIANGAn-xi1,LIUYang4,PENGYong-zhen1,2,SUNZhi-rong2(1.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,P.R.China;2.CollegeofEnvironmentandEnergyEngineering,BeijingInstituteofTechnology,Beijing100022,P.R.China;3.DepartmentofEnvironmentalEngineering,HarbinUniversityofCommerce,Harbin150076,P.R.China;4.ChinaWaterholdingPte.Ltd.,Beijing10022,P.R.China)Abstract:Inordertoinvestigatethevariationofsludgenitrificationactivitybeforeandaftermicro-bulkingwithlowdissolvedoxygen(DO),withmeanDOvalueof0.6mg/L-0.9mg/L,thesequencebatchreactor(SBR)wasemployedtotesttheoxygenconsumptionvelocitycurvesbeforeandaftermicro-bulking.TheresultsshowedthatCODremovalabilityincreased,whereastheammonianitrogenremovaleffectwasdecreasedwithmicro-bulkingofsludge.Theoxygenconsumptionvelocitycurvesbeforeandaftermicro-bulkingshowedthatthetotalactivityofactivatedsludgewas67.72mgO2/gVSS·hbeforemicro-bulking,ofwhichnitrificationactivitywas43.12mgO2/gVSS·h,thatis,63.67%ofthetotalactivity.Andthetotalactivityofactivatedsludgewas90.49mgO2/gVSS·haftermicro-bulking,ofwhichnitrificationactivitywas23.98mgO2/gVSS·h,thatis,26.51%ofthetotalactivity.ThelowDOvaluewasthelimitedsubstratesformicroorganismgrowth.Whenactivatedsludgewasinthestateofmicro-bulking,thefilamentousbacteriawerethedominantbacteria,whereasthenitrificationbacteriaweresubordinate.Thusthetotalnitrificationactivityofactivatedsludgedecreased.Keywords:lowDO;sludgemicro-bulking;nitrification;nitrificationbacteria;filamentousbacteria　　,[1-3]。,,[4-6]。(SVI)150mg/L,。[1],(DO)0.6～0.9mg/L,,(SVI)150～250mL/g,。,。,,,,,。,[7-8],,。1　1.1　,1。1　pH-6.5～8CODc/(mg·L-1)308～356NH4+-Nc/(mg·L-1)41～52PO43--Pc/(mg·L-1)3～51.2　2,。,,,700mm,200mm,12L。10cm,。,。,。pH、DO、ORP,。,25±1℃。、30min、3h、2h。,MLSS2000～2500mg/L,SRT=15d。SVI150～250mL/g。1　1.DO;2.pH;3.;4.;5.DO;6.pH;7.ORP;8.;9.ORP;10.;11.;12.;13.1.3[9]。COD5B-3COD;NH4+-N;MLSS、SS;DO、pH、ORPWTWinoLabOxilevel2。COD,。1.4　1.4.1　测定原理　JonnaSurmacz-Gorska[10],。,。,,,。NaClO3(ATU)。NaClO3,ATU。118土木建筑与环境工程　　　　　　　　　　　　　　　　第31卷1.4.2　测定装置　,。5,2。,,。。2　1.;2.DO;3.;4.;5.DO1.4.3　测定步骤　:1)(100mL);2);3),,,,;,;4)3mg/L,2.11g/LNaClO3,,,;5);2mg/L,5mg/LATU,;6)4)5),。2　2.1　SS2.1.1　反应器内生物相　,,,。,。3ab。,,,,。,。,,。,,。3　(×200)2.1.2　出水悬浮物(SuspendSolid,SS)　,SBR。,。SS1～2mg/L,,,SBRSS。,、,3,、,,,。2.2　CODCOD,2hCOD50mg/L。4,COD,COD30mg/L,COD6.5%～37.7%,,COD,COD。4　COD119第4期左金龙,等:低溶解氧污泥微膨胀前后污泥硝化活性的对比研究,2h5mg/L。5,,0.6～5mg/L,,,。5　45,COD,。,。2.3　。2。1NaClO3,,;2ATU,,。,1,2。。VSS,,3:=1×60/VSS(mgO2/gVSS·h)(1)=3×60/VSS(mgO2/gVSS·h)(2)=-6,1Y1=-0.2745x+6.6008,R12=0.9954(3),(VSS2432mg/L,100mL)SOUR1()=0.2745×60/0.2432=67.72(mgO2/gVSS·h)6　2Y3=-0.0997x+4.2334,R23=0.9948(4),:SOUR3=0.0997×60/0.2432=24.60(mgO2/gVSS·h)=SOUR1-SOUR3=67.72-24.60=43.12(mgO2/gVSS·h)2.4　7。1DO:Y1=-0.2621x+6.65,R12=0.9972(5),(VSS1732mg/L,100mL)SOUR1()=0.2612×60/0.1732=90.49(mgO2/gVSS·h)2:Y3=-0.192x+6.132,R32=0.9978(6),SOUR3=0.192×60/0.1732=66.51(mgO2/gVSS·h)=SOUR1-SOUR3=120土木建筑与环境工程　　　　　　　　　　　　　　　　第31卷90.49-66.51=23.98(mgO2/gVSS·h)7　2.5　,,8。8　8,90.49mgO2/gVSS·h,23.98mgO2/gVSS·h,26.5%;67.72mgO2/gVSS·h,43.12mgO2/gVSS·h,63.67%。,,,,,NH4+-N。2.6　,Monod:μ=μmaxSKs+S(7):μmax,d-1;S,mg/L;Ks,μ=μmax/2,mg/L。Chudoba[11],。μmax.1Ks.1,μmax.2Ks.2,,,9。9　DO,DO。DO,KDOKDO。,DO。,,。,COD,COD,;,,,。3　　1),COD,COD。,。2),67.72mgO2/gVSS·h,43.12mgO2/gVSS·h,24.60mgO2/gVSS·h;90.49mgO2/gVSS·h,23.98mgO2/gVSS·h,66.51mgO2/gVSS·h3)DO,,,,。:[1]MARTINSAMP,PAGILLAK,HEIJNENJJ,etal.Filamentousbulkingsludge-acriticalreview[J].WaterResearch,2004,38:793-817.121第4期左金龙,等:低溶解氧污泥微膨胀前后污泥硝化活性的对比研究[2]ALEJANDROH.CARAVELLI,LEDAGIANNUZ-ZI,NOEME.ZARITZKY.ReductionofhexavalentchromiumbySphaerotilusnatansafilamentousmicro-organismpresentinactivatedsludges[J].JournalofHazardousMaterials,2008,156(1-3):214-222.[3]MENGFAN-GANG,YANGFENG-LIN,XIAOJING-NI,etal.Anewinsightintomembranefoulingmechanismduringmembranefiltrationofbulkingandnormalsludgesuspension[J].JournalofMembraneScience,2006,285(1-2):159-165.[4]LIJIAN-FENG,LIYAO-ZHONG,DIEUDONN-GUYOHANDJA,etal.Impactoffilamentousbacteriaonpropertiesofactivatedsludgeandmembrane-foulingrateinasubmergedMBR[J].SeparationandPurificationTechnology,2008,59(3):238-243.[5]EDYTAF