低碳氮比猪场废水短程硝化反硝化厌氧氨氧化脱氮

21,2,3,13,1,2,1(11,610041;21,100049;31,610065):,SBBR2.,;,9118%9913%8411%,,111210124.,,;,,.:;;;:X713:A:025023301(2009)0320815207:2008203231;:2008207205:(KZCX32SW2442);(05SG0122001);(2008NG0006):(1978),,,,E2mail:wanghuan-cib@1631com3,E2mail:[e-mail]w2CPN2Piggery2WastewaterUsingShortcutNitrificationPdenitrification2ANAMMOXWANGHuan1,2,PEIWei2zheng3,LIXu2dong1,HAOChun1,2,ZENGKang2mei1(11ChengduInstituteofBiology,ChineseAcademyofSciences,Chengdu610041,China;21GraduateUniversityofChineseAcademyofSciences,Beijing100049,China;31SchoolofArchitectureandEnvironment,SichuanUniversity,Chengdu610065,China)Abstract:Aimingattheproblemofdeficiencyoforganiccarbonsourceinpiggerywastewatertreatmentbytraditionalmethod,shortcutnitrificationPdenitrification2ANAMMOXwasusedtoremovenitrogeninpiggerywastewaterwithlowCPNinSBBRreactors.TheresultsshowedthatshortcutnitrificationPdenitrificationprovidedgoodinfluentconditionsforANAMMOX.HighnitrogenremovalefficiencyofpiggerywastewaterbyANAMMOXwasachievedandtheaverageremovalpercentageofammonium,nitriteandtotalnitrogenreached9118%,9913%,8411%,respectively.TheperformanceofANAMMOXprocesswasnotaffectedbytheresidualorganiccompoundsinpiggerywastewater.Theaveragevariationratioofammonium,nitriteandnitratewas1112101241TheanalysisofGCPMSshowedthattherewaslittledifferencebetweentheorganiccompoundsininfluentandeffluentofANAMMOX,andmainorganiccompoundsweresaturatedhydrocarbonandester.Themicroorganismsystemwascomposedofanammoxbacteria,ammoniumoxidizer,nitriteoxidizeranddenitrificationbacteriatogether,andANAMMOXbacteriawasthemainfunctionalbacteriafornitrogenremovalinthissystem.Keywords:ANAMMOX;shortcutnitrificationPdenitrification;piggerywastewater;lowCPN,,,[1].,(CPN),.,[2].CPN,[3].(anaerobicammoniumoxidation,ANAMMOX)[4,5],,.,[68],,,COD,.,,Pathak[9]Chamchoi[10][11],,,30320093ENVIRONMENTALSCIENCEVol.30,No.3Mar.,2009,,CPN;Guven[12],,.,.CPN,,COD,,CPN.,,,,.1111,1.1Table1MainindexofpiggerywastewaterCODPmgL-1PmgL-1PmgL-1PmgL-1pH8001400400700120307137151,CPN2,.,[13],,.112(R1)(R2)SBBR(sequencingbatchbiofilmreactor),.,R150cm,8cm,2L;R252cm,13cm,6L,,.(1320)pH;,1L,24h;P,1520h,315610h,.302,pH;,3L,24h;,.,,1.12Fig.1SchematicdiagramofshortcutnitrificationPdenitrification2ANAMMOXprocess11311311COD:+(WC2I,);:HI2400(HannaInstrument);:(GBPT747921987);:N2(12)2(GBPT749321987);:(HJPT34622006);:(GBPT1189421989);pH:PHSJ23FpH(PHSJ23F,).11312(1):GC21690().:,:30mLPmin;:TDX201,3m3mm;:80,:60;:30.(2):SP21200().:,:011MPa;:TDX201,115m3mm;:90,:120,:120.11313P:HP6890P5973.:AB25MS(30m0125mm0125m).:(He)018mLPmin;260,:50,8Pmin280;:70eV;:20500u.11314DNADNA[14].6183011315PCR[1517].115%PCR.2211(20)pH,111,,[18]..(1),;COD(mg111mgCOD,COD,)50%42%;pH7138;.(2),11,100%;COD2415%;pH812718.(3),,COD6415%4911%,80120mgPL,pH718.,,,COD,11pH718.,,.21221211.SBR,.30d,,.21212,302pH.3.1:(080d),60mgPL,,;2:(80100d),,,100mgPL;3:(100140d),,,.23.2Fig.2VariationofsubstratesinsimulatedwastewaterbyANAMMOX3Fig.3VariationoftotalnitrogeninsimulatedwastewaterbyANAMMOX23,,11,,1mgPL,1015mgPL,20mgPL;,7811%9617%,99%,79%86%;111200124,[19].213302pH,,.1,46.7183:24Fig.4VariationofsubstratesinpiggerywastewaterbyANAMMOX5Fig.5VariationoftotalnitrogeninpiggerywastewaterbyANAMMOX6CODFig.6VariationofCODinpiggerywastewaterbyANAMMOX46,25d,80120mgPL,11,COD90140mgPL.,1mgPL,520mgPL,COD.,COD9118%9913%8411%1811%,111210124,,,.,,.214()(),(5mL,),.78.7Fig.7AnalysisongasproducedbyANAMMOXofsimulatedwastewater8Fig.8AnalysisongasproducedbyANAMMOXofpiggerywastewater,,,9419%9615%.,2,511%315%.,,,,.,2,.,,,,;,pH81830718815,,,.,,,.2156COD,,,.,(GCPMS),910.;22;;62;N2222;22212();;,9Fig.9Totalionicstreamofinfluent10Fig.10Totalionicstreamofeffluent910,,,.,,[912].,,,,,.21621611PCR()(),.,,:.16SrDNA,.11314DNA,,DNA15kb,.DNAPCR,115%,11.11Marker(DL2000);21;31;41;51;61;71;81Marker(DL2000)11DNAFig.11ResultofPCRofANAMMOXsludge11,.800bp,[15];9183:2130bp,[17].,,;.,;.,.21612MPN,MPN.MPN[20],2.2MPNPCFUg-1Table2CountingresultsofMPNPCFUg-1215105215105915103915104215105752,2,,.,,.,2,,.(),.216,,.,,,,;,,,.3(1),COD6415%4911%,pH71811,.(2),,COD9118%9913%8411%1811%,111210124,.(3),,9615%9419%,2.(4),,,.(5),,.:[1],.[M].:,2004126230.[2],,.[M].:,200411152116.[3],,,.[J].,2005,25():43246.[4]VanLuijnF,BoersPCM,LijklemaL.AnoxicN2fluxesfromfreshwatersedimentsintheabsenceofoxidizednitrogencompounds[J].WaterRes,1998,32(2):4072409.[5]VandeGraafAA,MulderA,DeBruijnP,etal.Anaerobicoxidationofammoniumisabiologicallymediatedprocess[J].ApplEnvironMicrobiol,1995,61(4):124621251.[6]YamamotoT,TakakiK,KoyamaT,etal.Novelpartialnitritationtreatmentforanaerobicdigestionliquorofswinewastewaterusingswim2bedtechnology[J].BioscienceandBioengineering,2006,102(6):4972503.[7]FuxC,BoehlerM,HuberP,etal.Biologicaltreatmentofammonium2richwastewaterbypartialnitritationandsubsequentanaerobicammoniumoxidation(anammox)inapilotplant[J].Biotechnol,2002,99:2952306.[8]GanigueR,LopezH,BalaguerMD,etal.Partialammoniumoxidationtonitriteofhighammoniumcontenturbanlandfillleachates[J].WaterRes,2007,41(15):331723326.[9]PathakBK,KazamaF,SaikiY,etal.Presence