低碳氮比猪场废水短程硝化反硝化厌氧氨氧化脱氮王欢

-1,2,3,1＊,1,2,1(1.,　610041;2.,　100049;3.,610065):,SBBR-.,;,、91.8%、99.3%、84.1%,,、、1∶1.21∶0.24.,,;,、,.:;;;:X713　:A　:0250-3301(2009)03-0815-07:2008-03-31;:2008-07-05:(KZCX3-SW-442);(05SG012-001);(2008NG0006):(1978～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]w-CN-Piggery-WastewaterUsingShortcutNitrificationdenitrification-ANAMMOXWANGHuan1,2,PEIWei-zheng3,LIXu-dong1,HAOChun1,2,ZENGKang-mei1(1.ChengduInstituteofBiology,ChineseAcademyofSciences,Chengdu610041,China;2.GraduateUniversityofChineseAcademyofSciences,Beijing100049,China;3.SchoolofArchitectureandEnvironment,SichuanUniversity,Chengdu610065,China)Abstract:Aimingattheproblemofdeficiencyoforganiccarbonsourceinpiggerywastewatertreatmentbytraditionalmethod,shortcutnitrificationdenitrification-ANAMMOXwasusedtoremovenitrogeninpiggerywastewaterwithlowCNinSBBRreactors.TheresultsshowedthatshortcutnitrificationdenitrificationprovidedgoodinfluentconditionsforANAMMOX.HighnitrogenremovalefficiencyofpiggerywastewaterbyANAMMOXwasachievedandtheaverageremovalpercentageofammonium,nitriteandtotalnitrogenreached91.8%,99.3%,84.1%,respectively.TheperformanceofANAMMOXprocesswasnotaffectedbytheresidualorganiccompoundsinpiggerywastewater.Theaveragevariationratioofammonium,nitriteandnitratewas1∶1.21∶0.24.TheanalysisofGCMSshowedthattherewaslittledifferencebetweentheorganiccompoundsininfluentandeffluentofANAMMOX,andmainorganiccompoundsweresaturatedhydrocarbonandester.Themicroorganismsystemwascomposedofanammoxbacteria,ammoniumoxidizer,nitriteoxidizeranddenitrificationbacteriatogether,andANAMMOXbacteriawasthemainfunctionalbacteriafornitrogenremovalinthissystem.Keywords:ANAMMOX;shortcutnitrificationdenitrification;piggerywastewater;lowCN　　,,、,[1].,、(CN),、.、、,[2].CN,[3].(anaerobicammoniumoxidation,ANAMMOX)、[4,5],,.,、[6～8],,,COD,.,,Pathak[9]、Chamchoi[10][11]、,,,30320093　　　　　　ENVIRONMENTALSCIENCEVol.30,No.3Mar.,2009DOI:10.13227/j.hjkx.2009.03.009,,CN;Guven[12],,、.,.CN,,COD、、,,、CN.,,,,.1　1.1　,1.1　Table1　MainindexofpiggerywastewaterCODmg·L-1mg·L-1mg·L-1mg·L-1pH800～1400400～700120～307.3～7.5　　1,CN2,.,[13],,.1.2　(R1)(R2)SBBR(sequencingbatchbiofilmreactor),.,R150cm,8cm,2L;R252cm,13cm,6L,,.(13～20℃)、pH;,1L,24h;,15～20h,3.5～6.0h,.30℃±2℃,pH;,3L,24h;,.,,1.1　-Fig.1　Schematicdiagramofshortcutnitrificationdenitrification-ANAMMOXprocess1.3　1.3.1　COD:+(WC-I,);:HI2400(HannaInstrument);:(GBT7479-1987);:N-(1-)-(GBT7493-1987);:(HJT346-2006);:(GBT11894-1989);pH:PHSJ-3FpH(PHSJ-3F,).1.3.2　(1)　:GC-1690().:,:30mLmin;:TDX-01,3m×3mm;:80℃,:60℃;:30℃.(2)　:SP-1200().:,:0.1MPa;:TDX-01,1.5m×3mm;:90℃,:120℃,:120℃.1.3.3　:HP68905973.:AB-5MS(30m×0.25mm×0.25×μm).:(He)0.8mLmin;260℃,:50℃,8℃min280℃;:70eV;:20～500u.1.3.4　DNADNA[14].816　　　　　　301.3.5　PCR、[15～17].1.5%PCR.2　2.1　(20℃)、pH,1.1,,[18]..(1),;COD(mg1.1mgCOD,COD,)50%、42%;pH7.38;.(2),1∶1,100%;COD24.5%;pH8.27.8.(3),,COD64.5%、49.1%,80～120mgL,pH7.8.,,,COD,1∶1、pH7.8.,,.2.2　2.2.1　.SBR、,.30d,,.2.2.2　,30℃±2℃、pH、.3.1:(0～80d),60mgL,,;2:(80～100d),,,、100mgL;3:(100～140d),,,.2、3.2　Fig.2　VariationofsubstratesinsimulatedwastewaterbyANAMMOX3　Fig.3　VariationoftotalnitrogeninsimulatedwastewaterbyANAMMOX2、3,,1∶1,,1mgL,10～15mgL,20mgL;,78.1%～96.7%,99%,79%～86%;、1∶1.20∶0.24,[19].2.3　30℃±2℃、pH、,,.1,4～6.8173:-4　Fig.4　VariationofsubstratesinpiggerywastewaterbyANAMMOX5　Fig.5　VariationoftotalnitrogeninpiggerywastewaterbyANAMMOX6　CODFig.6　VariationofCODinpiggerywastewaterbyANAMMOX4～6,25d,80～120mgL,1∶1,COD90～140mgL.,1mgL,5～20mgL,COD.,、、、COD91.8%、99.3%、84.1%、18.1%,、1∶1.21∶0.24,,,.,,.2.4　(Ⅰ)、(Ⅱ),Ⅰ、Ⅱ(5mL,),.7、8.7　Fig.7　AnalysisongasproducedbyANAMMOXofsimulatedwastewater8　Fig.8　AnalysisongasproducedbyANAMMOXofpiggerywastewater,Ⅰ、Ⅱ,,94.9%、96.5%.,2,5.1%、3.5%.,,,,.,2,.,,,,;,pH818　　　　　　307.8～8.5,,,.,,,.2.5　6COD,,,.,、(GCMS),9、10.①;②2-;③;④6-;⑤N--2-;⑥2--1-();⑦;⑧,9　Fig.9　Totalionicstreamofinfluent10　Fig.10　Totalionicstreamofeffluent9、10,,,.,,[9～12].,,,,,.2.6　2.6.1　PCR(Ⅰ)、(Ⅱ),.,,:、.16SrDNA,.1.3.4DNA,,DNA15kb,.DNAPCR,1.5%,11.1.Marker(DL2000);2.Ⅰ;3.Ⅰ;4.Ⅰ;5.Ⅱ;6.Ⅱ;7.Ⅱ;8.Marker(DL2000)11　DNAFig.11　ResultofPCRofANAMMOXsludge11,Ⅰ、Ⅱ.800bp,[15];8193:-130bp,[17].,,;.,;.,.2.6.2　MPN,MPN、.MPN[20],2.2　MPNCFU·g-1Table2　CountingresultsofMPNCFU·g-12.5×1052.5×1059.5×1039.5×1042.5×10575　　2,2,,.、,,.,、2,,.(),.2.6,,、.,,,,;,,,.3　(1),COD64.5%49.1%,pH7.8、1∶1,.(2),,、、、COD91.8%、99.3%、84.1%、18.1%,、、1∶1.21∶0.24,.(3)、,,96.5%、94.9%,2.(4),,,.(5),、、,.:[1]　,.[M].:,2004.26-30.[2]　,,.[M].:,2004.115-116.[3]　,,,.[J].,2005,25():43-46.[4]　VanLuijnF,BoersPCM,LijklemaL.AnoxicN2fluxesfromfreshwatersedimentsintheabsenceofoxidizednitrogencompounds[J].WaterRes,1998,32(2):407-409.[5]　VandeGraafAA,MulderA,DeBruijnP,etal.Anaerobicoxidationofammoniumisabiologicallymediatedprocess[J].ApplEnvironMicrobiol,1995,61(4):1246-1251.[6]　YamamotoT,TakakiK,KoyamaT,etal.Novelpartialnitritationtreatmentforanaerobicdigestionliquorofswinewastewaterusingswim-bedtechnology[J].BioscienceandBioengineering,2006,102(6):497-503.[7]　FuxC,BoehlerM,HuberP,etal.Biologicaltreatmentofammonium-richwastewaterbypartialnitritationandsubsequentanaerobicammoniumoxi