4612201412JOURNALOFHARBININSTITUTEOFTECHNOLOGYVol.46No.12Dec.2014庞长泷1,2,陈翰1,李昂1,马放1,21.1500902.21420010℃、.10.31%~16.89%25.07%~32.44%A10d、、CODCr2.433.076.023.63mg/L.X703.1A0367-6234201412-0045-08PerformanceofbioaugmentednitrogenremovalbyaerobicdenitrficationconsortiumfortreatingthedomesticsewageatlowtemperaturesPANGChanglong12CHENHan1LIAng1MAFang121.StateKeyLaboratoryofUrbanWaterResourceandEnvironmentHarbinInstituteofTechnology150090HarbinChina2.HITYixingAcademyofEnvironmentalProtection214200YixingJiangsuChinaAbstractLowtemperatureinhibitstheperformanceofnitrogenremovalefficiencyduringthebiologicaltreatmentofmunicipalwastewater.Thereforetheeffluentcouldhardlymeettheemissionstandard.Toenhancetheperformanceofnitrogenremovalatlowtemperatureapsychrotolerantaerobicdenitrificationconsortiumbyfastenrichmentanddomesticationwasobtainedwhichcouldremovetheammonianitrateandorganicmattersimultaneouslyandefficientlyinaerationenvironmentunder10℃.Theresultsshowedthattheremovalefficiencyofammoniumandtotalnitrogenbybioaugmentationwasimprovedby10.31%-16.89%and25.07%-32.44%respectively.Itmadetheeffluentqualitymeetthegrade1Alevelofnationaldischargestandard.Afterbioaugmentationterminatingfor10daystheremainedconsortiumcouldstillimprovethereactorthattheeffluentofammoniumnitratetotalnitrogenandCODCrconcentrationswasdecreasedabout2.433.076.02and3.63mg/Lrespectively.Theresultsindicatedthatbioaugmentationperformedwellandpersistently.Keywordslowtemperaturedomesticsewagenitrogenremovalbioaugmentationaerobicdenitrification2014-01-12.5110812051178139.1983—1963—.ang.li.harbin@gmail.com.、1.2-3.4-7、8-10..10℃SBR、.11.1.、、、111.2.0.018g/cm3100~120m2/g1~2mm2cm×2cm×2cm.14~16.1mg·L-1TOCpH80~1000~5140~160230~270750~9507.0~7.52mg·L-1CODCrpH100.0~300.035.0~55.00~0.50~3.040.0~60.06.8~7.21.210℃、、、3.0L1∶3.24h5min23h50min5min.SBR10℃.SBR、、、10.0L1.!!#$%!&!’!()*!#$%!+,-./’0//1234567&+,-./’&!#$%&()*&&!’$%1SBR1.31.3.130min0.6L2.4L.8000r/min10min、、、TOC3.、.1.3.2SBRSBR8h4.0L40%.10℃·64·46MLSS3000~3500mg/L20d.MLSSSV.A/O/A0.5h4h2h1h.、1min28min.、、.、.1.3.312.6~8d.2#1#..2~4mg/L...1.4CODCr、TOC、、、、、pH、DOMLSS13.1.5MicrosoftOfficeExcel2010OriginLabOrigin8.0.22.1TOC.TOC10.98mg/L...1192.03%39.98mg/L.10d10d.2.1082.85%21.09mg/L.!#!$#!%#!!###$#%#!#$’(!###$#%##%&’()’)!*%&+’*+,-./!0,-%&.-%&%&’()#&%$!#22d3.823.14mg/L0mg/L..14.12d、.2.210℃SBR45dCODCr·74·12.2.2.13.30.46mg/L.1710.62mg/L78.36%2184.78%6.43mg/L22~35d3681.09%6.56~8.65mg/L、...!#$%#$&’(!#$%$)*+,’(%)!#$#$&’(’*!+#$,’+-./01%,3SBR2.2.2..4SBR1~6d7~13d13d13.91mg/L1425.3910.71~11.25mg/L.!!#$%$!#$#!#’##!#$()%&’()(*+,-./$0*+%’()+%’()4SBR2.2.35.16d34.05~41.76mg/L17~36d21.87~31.55mg/L3761.29%18.54~19.76mg/LA.!#$%#$&’(!#$%&’’&%$)*+,()*!$&!+#$,(+-./01’,#$&’((25SBR3d·84·46CODCr88.27mg/LCODA4CODCr4169.97%CODCr39.07~46.23mg/LA.CODCr.SBR.2.3SBR2#SBR1#SBR.2.3.16.1#7.41~10.22mg/LA2#.9d2#7.87mg/L95.22mg/L2#11~30d2#3.01~4.21mg/L213.01mg/L..2116.89%272#93.35%.10.31%~16.89%.!##$%&’(#!#$)*%&’()*+,&’(*+-,&’(!+’(,)+-./01(,’(’’$’6SBR.2.3.21#2#9d90.45mg/L200mg/L..7.1#11.14~14.48mg/L2#.2#177.31mg/L1#248.22mg/L2#6.02mg/L281#12.03mg/L.2#15..!!#!$%&$!$#$’$!#$()%&’()(**+,-.!+,-.%&’()-./%&’()7SBR2.3.3·94·128.1#21.11~25.44mg/LA.2#.112#13.48mg/L16~30d2#10.77~11.76mg/L.1#44.04%~55.62%2#1575.19%1#50.38%2432.44%2#2778.59%.2#25.07%~32.44%.!#$###%&’&$#%(’($%!(%(!%!&%!#$)*!+%&,)+-./01(,’(%&)*+,(%&*+-,(%&8SBR272#.270.142g1#2#3.5013.062g/L2#4.127g/L0.9871.215mg·g-1·h-1.2#.1#CODCr41.44~51.62mg/LCODACODCr41.44mg/L25.2#CODCr146.93mg/L940.43mg/L1037.12~38.76mg/LCODCr37.12mg/L18.2#CODCr1#2#.CODCr2#CODCr8CODCr72.22%16CODCr76.55%CODCr8.18%.2.3.428.9、10.、CODCr0h40%0.5h0.5h.91#2#6.02~8.48mg/L..101#0.64mg/L2#CODCr2#2.58mg/L1#..·05·46.!#$%&&%$#!’()!#$*+!,%&-*,./012&-’()*%’()+,%’(),%()-*%()-+,%()-,%928!#!$#!%#!!###$#%###!&’%($)!#$*+)#$#(#%#’#!##!,%&-*,./012!-345*,./012!-’()%&()*%&’()345()*34510282.3.540.00mL+160.00mL、40.00mL+160.00mL+440.00mL+160.00mL+4500.00mL1、2、310℃150r/min.3d141.97±0.55mg/L38.15±0.17mg/L233.67±0.32mg/L326.28±0.59mg/L.123.2.430d2#10d.1#.2#.30、5103.2#1#2#10d2#1#、、CODCr2.433.076.023.63mg/L.3mg·L-1CODCr30d1#9.29±0.61012.99±2.9924.01±0.7942.26±7.162#3.95±0.2406.26±3.8211.42±0.1638.29±3.615d1#10.25±0.63012.58±0.8724.56±0.4948.57±1.592#5.98±0.1907.98±1.6415.17±0.5940.25±6.5110d1#8.64±0.31012.34±1.3422.71±0.2746.08±1.952#6.21±0.2709.27±2.4316.69±0.9542.45±2.31·15·12.3110℃、.21#2#10.31%~16.89%25.07%~32.44%A.310d.1#2#、、CODCr2.433.076.023.63mg/L.4.1.M.3.2007.2HEADMAOLESZKIEWICZJA.BioaugmentationfornitrificationatcoldtemperaturesJ.WaterResearch2004383523-530.3.J.200925182-8588.4.J.2008285885-891.5.SBRJ.2009298844-849.6.SBRJ.200733513-17.7.AOJ.200622884-88.8.J.2013332286-292.9.J.200941276-80.10.J.20102981567-1570.11YAOShuoNIJinrenCHENQianetal.EnrichmentandcharacterizationofabacteriaconsortiumcapableofheterotrophicnitrificationandaerobicdenitrificationatlowtemperatureJ.BioresourceTechnology2013127151-157.12.D.2011.13.M.4.2003.14TREUSCHAHLEININGERSKLETZINAetal.Novelgenesfornitritereductaseandamo-relatedproteinsindicatearoleofuncultivatedmesophiliccrenarchae