低温好氧颗粒污泥培养及其基质降解动力学王硕

44620126JOURNALOFHARBININSTITUTEOFTECHNOLOGYVol.44No.6Jun．2012111211．1500902．20009210℃25d、．COD、NH4+-N、PO43－-P84.5%、91.1%94.1%．MLSS10.61mg/gmPN/mPS1.57．EPS、．kKs5.49d－14760mg/L．TU991A0367－6234201206－0011－05CultivationofaerobicgranulesandsubstancesdegradationkineticsatlowtemperatureWANGShuo1SHIWen-xin1YUShui-li12YIXue-song11．StateKeyLaboratoryofUrbanWaterResourceandEnvironmentHarbinInstituteofTechnology150090HarbinChina2．StateKeyLaboratoryofPollutionControlandResourceReuseCollegeofEnvironmentalScienceandEngineeringTongjiUniversity200092ShanghaiChinaAbstractTheaerobicgranulesAGSweredifficulttocultivateandthedegradationkineticsofAGSatlowtem-peraturewereseldominvestigatedwhichgreatlylimitedthedevelopmentandapplicationofAGS．InthisstudyAGSpossessedbettersettlingpropertyandhigherbiomassaftercultivationbysodiumacetatefor25dfurthermoreAGSformationtimesignificantlydecreasedfor48dat10℃inasequencingbatchairliftreactorSBARandAGSpossessedbettersimultaneouslyCODNH4+-NandPO43－-Premovalefficiencyatlowtemperatureandtherespec-tiveremovalefficiencieswere84.5%91.1%and94.1%．ComparedwiththeconventionalcultivationofAGSthecontentoftotalproteinwasashighas10.61mg/gandthetotalproteins/totalpolysaccharidesmPN/mPSratiowas1.57whichmeantthattherelativehighproteincontentwasanessentialfeatureforAGSformation．TheadsorptionoforganicsubstancesbyAGSwashigherthanthatofflocsludgebecauseofthehighercontentsofEPSmorechannelsformassandoxygentransferaswellasthegreaterspecificsurfaceareaofAGS．TheexperimentaldataindicatedthatthesubstancesdegradationkineticsgenerallyfollowedMonodequationthecorrespondingkineticcoefficientofkandKswere5.49d－1and4760mg/Lrespectively．Thedegradationrateofsubstanceswasobvious-lyinfluencedbythemasstransferefficiencybetweenliquidphaseandAGS．KeywordslowtemperatureaerobicgranulesdegradationkineticsextracellularpolymericsubstancesEPS2011－08－18．2008AA06Z3042010DFA924602009ZX07424－005．1984—1962—[e-mail]．1、、2、3、45．Bao3Kreuk6．10℃．5℃．Frijters720%10%8．Liu9．．10．11．．11.1sequencingbatchairliftreactorSBAR6.0L50%0.15m3/h．、、．6h30min、30min、5min、5min290min．10℃．1.2mg/L625NH4Cl400KH2PO430K2HPO460MgSO440CaCl280EDTA40NaHCO3100COD、NH4+－N、TP1000、60、15mg/L．1mL/L8g/LFeCl3·6H2O1.5H3BO30.15CuSO4·5H2O0.03KI0.03MnCl2·4H2O0.12Na2MoO4·2H2O0.06ZnSO4·7H2O0.12CoCl2·6H2O0.15．A/O2.0g/L．1.3COD、NO2－-N、NO3－-N、NH4+-N、PO43－-PMLSS12．SVI3．ScanningElectronicMi-croscopeSEMS-4300NJapan．EPS13．1.414－1550mL150mLCOD600mg/L1hCOD．Qt=ρCOD0－ρCODt/ρMLSSQ=ρCOD0－ρCODt/t．Qtmg/gQ%ρCOD0ρCODt0tCODρMLSS．1.510－1150mL150mL250mLCOD1200、600、400、300、200mg/L1hCOD．22.1SBAR9d．SBARSBARBao3Liu16．25d、SEM1a．1b．SBAR·21·44SVI86.443.9mL/gMLSS1.84.7g/L2．aSEMbHE1SEMHESV1MLSS54321908070605040010203040t/d籽(MLSS)/(g·L-1)SVI/(mL·g-1)2SVIMLSS2.2EPSEPS、、、DNA13．3EPSMLSS3.8610.61mg/gMLSS5.298.60mg/gmPN/mPS0.741.57．EPSMc-Swain17Adav13Tay18EPS．Liu9．m(PN)/m(PS)PNPS111098765431.81.61.41.21.00.80.6010203040t/dw(PN,PS)/(mg·g-1)m(PN)/m(PS)3EPS2.3SBARCOD、NH4+-NPO43－-P．SBARCOD、NH4+-NPO43－-P．、SBARCOD、NH4+-NPO43－-P86.8、5.22.0mg/L4COD、NH4+-NPO43－-P84.5%91.1%94.1%．83.2%0.47mmol/L·h．Bao3Kreuk6COD、NH4+-NPO43－-P．2.453070.0mg/g30·31·655.0%．EPS31b19．30min．CODNH4+鄄NNO2-鄄NNO3-鄄NPO43+鄄P6050403020100600500400300200100060120180240300360t/min籽(N,P)/(mg·L-1)COD/(mg·L-1)4比污泥吸附量污泥吸附率100806040201008060402000102030405060t/min污泥吸附率/%比污泥吸附量/（mg·g-1）52.510－11．CODCOD1/V1/Sk=5.49d－1Ks=4760mg/L6V=S/0.8665+0.182S．kUnal20Guiot211011．、22－23．Ks2411．4.54.03.53.02.52.01.51.00.501234561/S/(L·g-1)1/V/(mg·d·g-1)y=0.8665x+0.182R2=0.9907k=5.49d-1Ks=4.76g/LV=S/(0.8665+0.182S)63125dCOD、NH4+-N、PO43－-P84.5%、91.1%94.1%．2EPSMLSS10.61mg/gEPS．3EPS、．30min70.0mg/g55.0%．4kKs5.49d－14760mg/L、．．1BEUNJJLOOSDRECHTvanMCMHEIJNENJJ．AerobicgranulationinasequencingbatchairliftreactorJ．WaterResearch2002363702－712．2MOYBYPTANGJHTOHSK．Highorganicload-inginfluencesthephysicalcharacteristicsofaerobic·41·44sludgegranulesJ．LetterAppliedMicrobiology2002346407－412．3BAORLYUShuiliSHIWenxin．Aerobicgranulesformationandnutrientsremovalcharacteristicsinse-quencingbatchairliftreactorSBARatlowtempera-tureJ．JournalofHazardousMaterials20091682/31334－1340．4SUNXFLIUCYMAY．EnhancedCuIIandCrVIbiosorptioncapacityonpolyethyleniminegraftedaerobicgranularsludgeJ．ColloidsSurfaceB2011822456－462．5XUHLIUYTAYJH．EffectofpHonnickelbiosop-tionbyaerobicgranularsludgeJ．BioresourceTech-nology2005973359－363．6KREUKdeMKPPONKMLOOSDRECHTvanMCM．Formationofaerobicgranulesandconversionproces-sesinanaerobicgranularsludgereactoratmoderateandlowtemperatureJ．WaterResearch200539184476－4484．7FRIJTERSCTMJEIKELBOOMDHMULDERA．Treatmentofmunicipalwastewaterinacircoxairliftre-actorwithintegrateddenitrificationJ．WaterScienceTechnology1997361173－181．8SHIWenxinWANGShuoYIXuesongetal．Contam-inantsremovalcharacteristicsbetweenflocsludgeandaerobicgranularsludgeindomesticsewagetreatmentatlowtemperatureC//InternationalSymposiumonWa-terResourceandEnvironmentalProtection．Xi’ans．n．20111351－1354．9LIUYTAYJH．TheessentialroleofhydrodynamicshearforceintheformationofbiofilmandgranularsludgeJ．WaterResearch20023671653－1665．10．J．200838395－98．11．J．20071231