37720117JOURNALOFBEIJINGUNIVERSITYOFTECHNOLOGYVol.37No.7Jul.2011100124SBRSVI150~200mL/g.FISHMPA223.CODPO34-P-80%98%NH+4-NTN52%28%SND15%SND.X703.1A0254-0037201107-1085-052009-05-31.KM200910005003PYZZ090429002183.1973—.、1-3.———4.、、5、..SBR.11.1SBR1.7L/anoxic/oxic1.5h2.5h0.2kg/kg·d50%.ρDO13±1℃.ρMLSS3000mg/L.1pH=7.0~7.8.20111Table1Wastewatercharacteristicmg/LρCODρNH+4-NρNO-3-NρNO-2-NρPO3-4-P160~28058~780.13~1.10.04~0.244.0~6.81.21.2.3.4.5.6.1SBRFig.1SchematicdiagramoftheSBRρCOD、ρPO3-4-P、ρNH+4-N、ρNO-3-N、ρNO-2-N、ρMLSS、SVIρTNρNH+4-N、ρNO-3-NρNO-2-N.ρDOWTWMulti340i.OLYMPUS2BX51Eikelboom6.1.3FISHfluorescentinsituhybridizationFISH、、7.FISHCy3MPA223OLYMPUSDP71.22.12400Fig.2MorphologicalcharacteristicsofsludgeflocundernormalDO400timesSBRρDO2mg/LSVI90~110mL/g、2.ρDO2.0mg/L0.5mg/LSVIρDO5d166mL/g3.37dSVI167~210mL/g4.0.5hSBRSS.SBR.680173400Fig.3MorphologicalcharacteristicsofsludgeflocunderlowDO400times4SVIFig.4FluctuationofSVIduringtheexperiment2.2.、.Eikelboommicrothrixparvicella5.MPA223FISHMPA2236.51000Fig.5ThepicturesoflimitedfilamentousbulkingsludgebyGramstaining1000times6MicrothrixparvicellaMPA223FISH1000Fig.6ThepicturesofMicrothrixparvicellaMPA223inlimitedfilamentousbulkingsludgebyFISH1000times640dSVI.200~78012011500μm“”“”..A/OA2/O..0.5h8、.2.37Fig.7TheeffectoflowDOtotheremovalrateofpollutants7ρDOSBRCOD、PO3-4-P、NH+4-NTN.ρDOCODρDOCOD.ChudobaKSρDOCOD.ρDOPO3-4-P98%ρPO3-4-P0.ρDOρNO-xNO-x.45d52%30%..SND15%SND.22℃10~15℃SND.31SBR13±1℃ρDO0.5mg/L0.2kg/kg·dSVI160~210mL/g.2FISHMPA223200~500μm“”SVI.3、COD、PO3-4-P80%98%.NH+4-NTN52%28%15%SND.88017.1MARTINSAPAGILLAKHEIJNENJJetal.Filamentousbulkingsludge-acriticalreviewJ.WaterRes200438793-817.2EIKELBOOMDH.ProcesscontrolofactivatedsludgeplantsbymicroscopicinvestigationM.LondonUKIWAPublishing2000.3.M.1992.4.、J.200829123342-3347.PENGYong-zhenGUOJian-huaWANGShu-yingetal.EnergysavingachievedbylimitedfilamentousbulkingunderlowdissolvedoxygenderivationoriginalityandtheoreticalbasisJ.EnvironmentalSicence200829123342-3347.inChinese5SEZGINMJENKINSDPARKERDS.AunifiedtheoryoffilamentousactivatedsludgebulkingJ.WatPollControl197850362-368.6EIKELBOOMDH.FilamentousorganismsobservedinactivatedsludgeJ.WaterRes19759365-388.7.MBRJ.200425656-58.SUNYu-jiaoWANGYongHUANGXia.MoleculeecologyanalysisoffilamentousbacteriumsludgebulkinginMBRJ.EnvironmentalSicence200425656-58.inChinese.8.J.200134279-82.CUIHong-shengBAIXiao-huiLIGangetal.SludgebulkinganditscontrolincoldregionJ.JournalofHarbinUniversityofCE&Architecture200134279-82.inChineseInitiationandMoleculeEcologyAnalysisofLowDissolvedOxygenLimitedFilamentousBulkingatLowTemperatureGAOChun-diWULian-juHAOKunLILeiYUANJin-pingCollegeofEnvironmentalandEnergyEngineeringBeijingUniversityofTechnologyBeijing100124ChinaAbstractActualdomesticsewagewastreatedbySBRprocess.LimitedfilamentousbulkingwasinducedbylowdissolvedoxygenatlowtemperatureandtheSVIweremaintained150-200mL/g.TheoccurrenceoflimitedfilamentousbulkingatlowtemperatureandthegrowthofmicroorganismwereresearchedthetechniqueofFISHwasadoptedtoidentifythespeciesofthedominantfilamentousbacteriainthestateoflimitedfilamentousbulkingandtheresultshowedthatthefilamentousbacteriathatledtolimitedfilamentousbulkingwasmicrothrixparvicellaMPA223.ComparedtothestatusatnormaldissolvedoxygentheremovalrateofCODandPO3-4-Pincreasedto80%and90%whiletheremovalrateofNH+4-NandTNdecreasedto52%and28%respectivelyduringlimitedfilamentousbulking.Andthedataalsoshowedthatlowdissolvedoxygenledtothephenomenonofsimultaneousnitration-denitrationSNDabout15%ofthenitrogenwasremovedbySND.Keywordslowdissolvedoxygenlimitedfilamentousbulkinglowtemperaturemicrothrixparvicella9801