低温下A2O工艺污泥膨胀的微生物群落结构变化王秀朵

A2/O123121244451．3000512．3000513．3001914．3003845．300072A2/O。21℃—19℃—12℃SVI、COD。αCandidatedivisionTM7。A2/O。A2/OX703[e-mail]ctureChangeinanA2/OActivatedSludgeBulkingatLowTemperatureWANGXiu-duo12QINLi3PANLiu-ming12ZHAOLe-jun12CHENGeng-ni4FENGZhi-tong4DUANZheng-hua451．TianjinMunicipalEngineeringDesignandＲesearchInstituteTianjin300051China2．TianjinEnterpriseKeyLaboratoryofInfrastructureDurabilityTianjin300051China3．Agro-EnvironmentalProtectionInstituteofMinistryofAgricultureTianjin300191China4．SchoolofEnvironmentalScienceandSafetyEngineeringTianjinUniversityofTechnologyTianjin300384China5．SchoolofEnvironmentScienceandEngineeringTianjinUniversityTianjin300072ChinaAbstractThemechanismsandcontrolmeasuresofactivatedsludgebulkinginananaerobic－an-oxic－oxicA2/Oreactoratlowtemperaturewerestudiedusingthewaterqualityparameterdetectingandthehighthroughputsequencingtechnology．Ｒesultsshowedthatinthe21℃－19℃－12℃cool-ingprocessthesludgevolumeindexSVIvaluewassignificantlyincreasedandtheCODremovalratewasdecreasedcorrespondinglyandthemicrobialcommunitystructureintheaerationtankwassignifi-cantlychangedaswell．ThedominantpositionofbacteriathatlackofresistancetolowtemperatureswasreplacedbyAcidobacteriameanwhileAlphaproteobacteriaandCandidatedivisionTM7hadalsopropa-gatedsignificantly．Thereforetheactivatedsludgebulkinginthisreactorwasmainlyattributedtothechangesinthemicrobialcommunitystructureatlowtemperatures．Theeffectoflowtemperatureonthe·401·331320177CHINAWATEＲ＆WASTEWATEＲVol．33No．13Jul．2017activityofA2/Oreactorwasreversible．Thesludgecouldrecovertoagooddegradationeffectaftersomecontrollingmeasureswereimplemented．KeywordsA2/Oprocessactivatedsludgebulkinglowtemperaturemicrobialcommunitystructurefilamentousbacteria、、。12。、345。。、67。A2/O。11.1A2/O1、、。1Fig．1Diagramofexperimentalsetup300L5125L225L3450L5200L。20L/h20h22．50L/h7．50L/h。1.2COD、TNTP75%、25%、80%COD、TNTP600、40、6mg/L。1.3。21℃。1/321℃。1.4O-MEGADNADNA。DNA16SrDNAV4。silva119a-ligned16S/18SSSUOTU。22.12.1.1COD、COD2。COD600mg/L。7～9d19℃COD。10～2512℃COD40%18。26～401/321℃316dCOD150mg/L。12℃·501·．watergasheat．comA2/O3313。A2/O。8。2CODFig．2ChangeofCODconcentrationineffluentandinfluent2.1.2SVIMLSS、MLVSSSVI3。19～21℃MLSS、MLVSSSVI。19℃12℃MLSSMLVSS1400mg/L900mg/LSVI20～24dSVI150mL/g。2COD。3SVIFig．3ChangeofsludgeconcentrationandSVIvalue。12℃21℃282500mg/LSVI30d。2COD。2.22.2.121℃4、19℃8、12℃20、、20406。635000、30904、45092、47936、2842434589400～500bp。。OTUOTU4。20。A2/O21℃。4Fig．4Wholesamplesimilarityanalysis2.2.25。Proteobacteria2Acidobacteria、ChloroflexiBacteroidetes。21℃—19℃—12℃。、Verru-comicrobia32．60%—31．36%—27．43%。·601·3313．watergasheat．comGemmatimonadetes、Actinobacteria、Planctomycetes、CandidatedivisionTM7Synergistetes。。。5Fig．5Ｒelativeabundancesofdifferentphyla2047．27%25．27%。。SVI。2.2.3OTU6。10℃9。1011。6Fig．6ＲelativeabundancesoffilamentousbacteriaαAlphaproteobacteria、AnaerolineaeSaprospiraceaeα。CandidatedivisionTM7。αCandidatedi-visionTM7。3①A2/OSVI、COD。②A2/OCandidatedivisionTM7、α。1．、J．200829123342－3347．2．A/OJ．20153672610－2616．112·701·．watergasheat．comA2/O3313－1572．7．J．2016321336－40．8．CODICJ．20163561305－1310．9．J．20116292629－2633．10WuJHLinCY．BiohydrogenproductionbymesophilicfermentationoffoodwastewaterJ．WaterSciTechnol200495/6223－228．11．J．2012313307－312．12．pHUASBJ．2016354810－816．13LeeHSＲittmannBE．EvaluationofmetabolismusingstoichiometryinfermentativebiohydrogenJ．Biotechn-olBioeng20091023749－758．1984－、。[e-mail]櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆2016－12－231073．J．20143583031－3037．4．J．2015311535－39．5．J．2001215569－572．6．BIOLAKJ．20153651739－1748．7．J．2015431189－191．8．SBＲJ．201127791－94．9．A2OJ．20133472912－2917．10KragelundCLevantesiCBorgerAetal．Identitya-bundanceandecophysiologyoffilamentousbacteriabel-ongingtotheBacteroidetespresentinactivatedsludgeplantsJ．Microbiology20081543886－894．11．FISHJ．2012184705－712．1960－、、。[e-mail]2016－12－20·211·3313．watergasheat．com