低温下活性污泥膨胀的微生物群落结构研究端正花

37320163ENVIＲONMENTALSCIENCEVol．37No．3Mar．20161231232311．3003842．3000513．300051．SVI1～412．．SaprospiraceaeFlavobacterium．．X172X171.5A0250-3301201603-1070-05DOI10．13227/j．hjkx．2016．03．0362015-09-082015-10-2051103161981～[e-mail]unityStructureinActivatedSludgeBulkingatLowTemperatureDUANZheng-hua1PANLiu-ming23CHENXiao-ou1WANGXiu-duo23ZHAOLe-jun23TIANLe-qi11．SchoolofEnvironmentalScienceandSafetyEngineeringTianjinUniversityofTechnologyTianjin300384China2．TianjinMunicipalEngineeringDesignandＲesearchInstituteTianjin300051China3．TianjinEnterpriseKeyLaboratoryofInfrastructureDurabilityTianjin300051ChinaAbstractThemechanismofactivatedsludgebulkinginZhengzhouwastewatertreatmentplantwasstudiedbymeasurementofwaterqualityparametersandhigh-throughputsequencingtechnology．ThechangeofSVIvaluewassignificantlynegativelycorrelatedwiththeseasonaltemperaturevariationandsludgebulkingwaseasytooccurduringDecembertothenextAprilbutthewaterqualitywasnotaffected．Theresultverifiedbyhigh-throughputsequencingtechnologyanalysisshowedthatthemicrobialcommunitystructureofbulkingsludgewassignificantlydifferentfromthatofthenon-bulkingone．ThedominantfilamentousbacteriainthebulkingsludgeinthisplantwereSaprospiraceaeandFlavobacterium．Thereforetheactivatedsludgebulkinginthiswastewatertreatmentplantwascausedbythepropagationoffilamentousbacteriaatlowtemperature．Keywordslowtemperatureactivatedsludgebulkinghigh-throughputsequencingmicrobialcommunitystructurefilamentousbacteria、．60%1～34～6．50%795%89．10～12．、1314．．．2014．11.1A2/O30m3·d－11.30．BOD520mg·L－1COD80mg·L－1．14dBOD50.08kg·kg·d－13.80g·L－10.97kg·kg－112℃．3．COD．A2/O．－20℃．1.2OMEGADNAE．Z．N．A．TMSoilDNAKitOMEGAD5625-01USADNA．DNA．DNA50μLTEbuffer－20℃．1CODFig．1CODchangesofeffluentandinfluent30μLDNA16SrDNAV4Ｒoche454GSFLXsequencer．silva119aligned16S/18SSSUOTU．mothurhttp//．mothur．org/chao1ACEShannon．22.12.1.1COD2014112COD1COD339mg·L－1．22.40mg·L－150mg·L－1COD90%．1～414～20℃COD90%COD．．158～20℃SUFＲSUFＲCOD86%．16310.40～17.10℃A/OCOD82.10%．17．2.1.2SVI20142SVI．1SVI1502502250．34．512SVI100．81、2、3、4、12．1819．Knoop2012℃．．2.22.2.161、2、3、1701372SVIFig．2ChangeofSVIvaluethroughouttheyear4、81268194、53100、61602、68481、3353265724．400～500bp．rarefactioncurve．PC-OＲDPCA．38．3PCAFig．3Principlecomponentanalysis438．．．2.2.297%OTU4Fig．4Wholesamplesimilarityanalysisoperationaltaxonomicunits．30．5．81、2、3、4、12．．Proteobacteria1、2、3、4、81250.18%、43.77%、43.24%、43.58%、36.15%44.39%．Bacteroidetes815.66%．Chloroflexi．Actinobacteria270135Fig．5Ｒelativeabundancesofdifferentphyla1%88.50%．、、．SVI．2.2.3OTU．68．Saprospiraceae1、2、3、4、1250%825%SVIFlavobacteriales．SaprospiraceaeFlavobacteriales．AnaerolineaeCandidatusMicrothrix21228．2324．．．、2526．．6Fig．6Ｒelativeabundancesoffilamentousbacteria31．2SaprospiraceaeFlavobacteriales．1．J．20051520172-173．2．4J．37013720123351646-1651．3．CANONJ．20153682939-2946．4．、J．200829123342-3347．5．J．2010361143-45．6．A/OJ．20153672610-2616．7．A2OJ．20088121074-1078．8．SBＲJ．2000202129-133．9．J．2006751-9．10．J．2008356939-943．11．J．201329383-86．12．J．20143583031-3037．13．J．2015311535-39．14．J．2001215569-572．15．SUFＲJ．2005254512-518．16．D．2014．22-23．17．J．20113771085-1089．18．J．20031995-7．19．MBＲJ．2009301155-159．20KnoopSKunstS．InfluenceoftemperatureandsludgeloadingonactivatedsludgesettlingespeciallyonMicrothrixparvicellaJ．WaterScienceandTechnology1998374-527-35．21．J．20123181151-1156．22．A2OJ．20133472912-2917．23KragelundCLevantesiCBorgerAetal．IdentityabundanceandecophysiologyoffilamentousbacteriabelongingtotheBacteroidetespresentinactivatedsludgeplantsJ．Microbiology2008154Pt3886-894．24．FISHJ．2012184705-712．25．J．200724229-33．26．SBＲJ．201127791-94．4701