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102Vol．10No．220162ChineseJournalofEnvironmentalEngineeringFeb．2016EGSB1111*2211．4000452．401122NaClw/v。11h8g/L。125d107d73．0±13．9%78．4±3．7%。。。。7EPS、。EPS2．83．1。EGSB、。EGSBX703A1673-9108201602-0524-07Effectoflowsalinityonstart-upandoperationofAnammoxprocessinEGSBreactorZengQiansong1WangHan1ZhouWeiyi1FangFang1ChenYoupeng2GuoJinsong2LiuLianwei11．FacultyofUrbanConstructionandEnvironmentalEngineeringChongqingUniversityChongqing400045China2．ChongqingInstituteofGreenandIntelligentTechnologyChineseAcademyofSciencesChongqing401122ChinaAbstractEffectoflowsalinityNaClw/vonstart-upandoperationofAnammoxinEGSBreactorwasinvestigatedinthiswork．Twoidenticalreactorswerestartedandoperatedwhilehydraulicretentiontimewas11hoursineachreactor．Normalactivatedsludgewasappliedasinoculatedsludge．Differentsalinities8and0g/Lwereappliedineachreactor．Ｒesultsindicatethatthestart-upofthetworeactorscouldbeachievedsuccess-fullyin125daysand107daysrespectively．Inadditiontheremovalefficienciesoftotalnitrogeninthetwore-actorswere73．0±13．9%and78．4±3．7%respectivelywhilethepresenceoflowsalinityresultedinlowerremovalefficiencyduringtheoperation．Thesizeofmediandiameterincreasedwiththeincreasingofrefluxratioinbothtworeactors．Howeverthepresenceoflowsalinityreducedthesizeofmediandiametercomparedtothecasewhilenosalinitywasapplied．Whiletherefluxratiowassevenlowsalinityresultedinlesspolysac-charideproteinandhumus．Inadditiontheratiosofpolysaccharideandproteinofthetworeactorswere2．8and3．1respectively．Thepresenceoflowsalinityaffectedthestart-upoperationandtheformationofgranularsludgeinEGSBreactor．KeywordsEGSBAnammoxsalinitygranularsludge51278509511084822014－10－142014－11－241988—。E-mail449700248@qq．com*E-mailfangfangcq@cqu．edu．cnanaerobicammoniumoxidationAn-ammoxNH+4NO－2N211。NH+4+1．32NO－2+0．66HCO－3+0．13H+→1．02N2+0．26NO－3+2．03H2O+0．066CH2O0．5N0．15AnammoxAna-mmox202EGSB、、。Anammox2、、、、。。Anammox。Kuypers3CandidatusScalinduasorokinii。4UASB。Toh5Waki6Anammox。7、。Ana-mmoxexpanded-granularsludgebedEGSBAnammoxAnammox。Anammox。EGSBEGSBextracellularpolymersEPSAnammox。11．11#2#EGSB1。7cm85cm3L105cm6L。Anammox。。10～15min。33±1℃。pH7．5～8．0HＲT11h。1EGSBFig．1SchematicdiagramofAnammoxEGSBreactor1．2NH+4-NNO－2-NNH4ClNaNO21∶11KHCO3HCO－3NH+42∶1。KH2PO40．025g/L、CaCl2·2H2O0．2g/L、MgSO4·7H2O0．2g/LI、II1．25mL/L。IEDTA5g/LFeSO45g/LIIEDTA15g/LZnSO4·7H2O0．43g/LCoCl2·6H2O0．24g/LMnCl2·4H2O0．99g/LCuSO4·5H2O0．25g/LNaMO4·2H2O0．22g/LNiCl2·6H2O0．19g/LNaSeO4·10H2O0．21g/LH3BO40．014g/LNaWO4·2H2O0．05g/L。1#8g/L2#1#52510。MLSSMLVSS69．06g/L25．05g/L2L。1．311#2#EGSBEPS。1．4-8N-1--88EPS-9Folin10MicrotracS3500。1EGSBTable1OperationschemeforEGSBdNH+4-Nmg/LNO－2-Nmg/Lkg/m3·dm/h1～9858580．2500．1498～12598980．4300．14125～1601681680．7300．14160～1751681680．7340．71175～2051681680．7350．85205～2341681680．7371．1322．1EGSB234d1#125d109d2#107d127d。Ⅰ～ⅢⅣ。2。2TNFig．2VariationofconcentrationsofammoniumnitritenitrateTNandTNremovalefficiency2．1．1EGSB3。Ⅰ1～16d。。。41112。Ⅰ8g/L6252EGSB8g/L。1#Ⅱ16～83d2#16～62d。5%。Ⅰ。11d13-15。Strous161010～1011cell/mL。1#2#21d8g/L8g/L。1#Ⅲ83～125d2#62～107d。1#8312．7%12578．7%2#6219．7%10783．7%。10058mg/L98mg/L10132．4%35．7%。1#2#70%73．0±13．9%78．4±3．7%NH+4-NNO－2-NNO－3-N1∶1．20±0．22∶0．32±0．091∶1．19±0．07∶0．23±0．031∶1．32∶0．26。。。III42d45d2#1#8g/L。2．1．2EGSB125d98mg/L168mg/L3d27．6%21．8%。160d18．2%16%。0．14m/h。160、1752054、57TN。。233d70%83%1#2#。8g/L8g/L。3Fig．3MediangranularsizeintheAnammoxreactoratdifferentrefluxratios2．2EGSB2．2．1EGSB3D5050%7251050%。3125d1#2#D5059．37μm99．88μm2#D501#。D502#1#220d71#2#146．4μm264．1μm。。31#D502#8g/L。17183～15g/L。Ma19。。18-20621。。EGSBSBＲUASB4172223EGSB。EGSB。2．2．2EGSBEPSEPS、、、、DNA24。EPS。25EPS。EGSBEPS、、EPS。4a4b234、EPSEPS、。4EPS234dFig．4ContentofEPSinEGSBreactor234d42#、1#。EPS。26EPS。27EPS。EPSEPS。1#2#EPSPS/PN2．83．1。8252EGSBPS/PN0．9PS/PN3．4～6．225。EPS25。EPS28。29。EPS。8g/L。318g/L1#2#。1#2#125d107d73．0±13．9%78．4±3．7%。1#2#。8g/L、。2。1#2#。8g/L。371#EPS、2#2．83．1。EPS。1StrousM．KuenenJ．G．JettenM．S．Keyphysiologyofanaerobicammoniumoxidation．AppliedandEnvironmentalMicrobiology19996573248-32502JaroszynskiL．W．CicekN．SparlingＲ．etal．Impor-tanceoftheoperatingpHinmaintainingthestabilityofan-oxicammoniumoxidationAnammoxactivityinmovingbedbiofilmreactors．BioresourceTechnology2011102147051-70563KuypersM．M．M．SliekersA．O．LavikG．etal．AnaerobicammoniumoxidationbyAnammoxbacteriaintheBlackSea．Nature20034226932608-6114．UASB．2013331215-20FangFangBaoZhenguoChenYoupengetal．Start-upandoperationoftheAnammoxprocessundersalinitycondi-tionsinUASBreactor．IndustrialWaterTreatment2013331215-20inChinese5TohS．K．AshboltN．J．Adaptationofanaerobicammo-nium-oxidisingconsortiumtosyntheticcoke-ovenswastewater．AppliedMicrobiologyandBiotechnology2002592-3344-3526WakiM．TokutomiT．YokoyamaH．etal．Nitrogenre-movalfromanimalwastetreatmentwaterbyAnammoxenrich-ment．BioresourceTechnology200798142775-27807．．2010303504-512JinＲencunYangGuangfengMaChunetal．Effectofacetateandinorganicsaltsontheperformanceofapartialnitritationreactor．ActaScientiaeCircumstantiae2010303504-512inChinese8．4．20029GaudyA．Colorimetricdeterminationofproteinandcarbo-hydrate．IndustrialWaterWastes1962717-2210JinBoWilénB．M．LantP．Impact