37720117JOURNALOFBEIJINGUNIVERSITYOFTECHNOLOGYVol.37No.7Jul.2011100124sequencingbatchreactorSBRaerobicgranularsludgeAGSAGS.ρCOD600~900mg/L10dAGS1.15±0.14mmSVI90mL/gAGS23dρCOD900mg/L1200mg/LAGSAGSΔ1.69±0.23mmSVI175mL/g.AGS+1∶1AGS34dAGS“”AGSΔ1.00±0.01mm.ρCOD600mg/L1200mg/LAGS.AGSAGSAGS.X703.1A0254-0037201107-1027-062009-07-25.510780078112007PHR20110819.1974—.aerobicgranularsludgeAGS、、、.AGSAGS.Tay1AGSAGSAGS2Zheng3AGS6kgCOD/m3·d30dAGSAGS.、AGSAGSAGSAGS.AGS.AGS.sequencingbatchreactorSBRAGSAGS+AGS.11.1NH4Cl229.3mg/LKH2PO443.9mg/LMgSO4·7H2O90mg/LCaCl2·2H2O14mg/L0.4mL/L.FeCl3·6H2O1.5gH3BO30.15gCuSO4·5H2O0.03gKI0.18gMnCl2·H2O0.12gNa2MoO4·2H2O0.06gZnSO4·7H2O0.12gCoCl2·6H2O0.15gEDTA10g.ρCOD600mg/LρMLSS2173mg/L.20111.2SBRD=9cmH=90cmH/D=106L.0.12m3/h25±1℃2min290min5min2min1min.66%.4.1.3SV、COD、NH+4-N、MLSSOlympusBX51CANONIX850AGS、、、、S-4300AGSDdAGSΔ=D/d4ΔAGSIm-age-proplus6.0.1Fig.1Changesinsubstratetypeandconcentrationduringtheexperiment1.41.5AGSS11~10d、AGSS211~23d、AGSS324~43d、AGSS444~77d、AGSS578~120d.22.1SBRAGS2AGSS1~S2.ρCOD600mg/L900mg/L10d2b.AGSS20.8~1mm.2AGSS1、S2Fig.2Imagesofsludgeinthegranulationprocessphase1and22.2AGSρMLSSAGSρCOD900mg/L1200mg/LS3.AGS.3ρMLSSSVI3AGSS324~43dρMLSS820173ρMLSSSVIFig.3ChangesinMLSSandSVIinaerobicgranularsludgeSBR213mg/LSVI60mL/g175mL/g.15min“”AGS“”.4S3AGS.4AGS25d43dΔ1.45±0.1425d1.69±0.2343d.AGSEikelboom55Fungispp.4AGSS3Fig.4Morphologychangesofthefilamentousaerobicgranularsludgeinphase35Fig.5GramandNeisserstainofthefilamentousbacteria2.3AGSAGSAGSAGSρMLSSSVI.AGS+S4ρCOD600mg/L300mg/L.S4AGSAGS.6S4AGS.6AGSΔ1.69±0.2343d1.41±0.1953d69dΔ1.29±0.08.34dAGSAGS.6AGSS4、S5Fig.6Morphologychangesoftheaerobicgranularsludgeinphase4and5S5AGS2.5~3.0mm7.8S5AGSAGSρCOD1200mg/L600mg/LS3ρCODAGS.920120117Fig.7Changesinsizeofaerobicgranularsludge8S5AGSFig.8TheSEMimagesoftheaerobicgranularsludgeinphaseS53SBRAGSρCOD600~900mg/L10dAGS1.15±0.14mmSVI90mL/gAGS23dρCOD900mg/L1200mg/LAGSΔ1.69±0.23mmSVI175mL/gAGS+1∶1AGS34dAGS“”Δ1.00±0.01mm.、DO、、、pH.AGS16.、AGS.7AGS.2ρCOD600mg/LS1~S21200mg/LS32.4kgCOD/m3·d4.8kgCOD/m3·dDO2mg/L.ρCODAGS/8.3.S1—S3.4pHpH6.7pH.AGS、.S4AGS+1AGS“”AGS.2ρN/ρCOD9-10ρN/ρCODAGS1ρN/ρCODS1—S3ρCODρN/ρCODAGS.S4214.5%.1ρN/ρCOD030171ρN/ρCODTable1ChangesintheratioofN/CODduringtheexperimentρCOD/mg·L-1ρN/ρCOD60010∶100S1~S39006.7∶10012005.0∶10060017.3∶100S4~S5+90013.9∶100120012.3∶100“”9S4—S544~120d25%9Fig.9Theeffluentammonianitrogenandtheremovalofammonianitrogeninthereactor70%S3AGSAGSAGS.S3S4ρDOAGSDOAGS.41SBRAGSρCOD600~900mg/L10dAGS1.15±0.14mmSVI90mL/gAGS23dρCOD900mg/L1200mg/LAGSΔ1.69±0.23mmSVI175mL/g+1∶1AGS34dAGSΔ1.00±0.01mm.2AGSρN/ρCOD+AGSρN/ρCODAGSAGSAGS.1TAYJHLIUQSLIUY.AerobicgranulationinsequentialsludgeblanketreactorJ.WaterScienceandTechnology2002464-513-18.2TAYJHLIUQSLIUY.MicroscopicobservationofaerobicgranulationinsequentialaerobicsludgeblanketreactorJ.JournalofAppliedMicrobiology2001911168-175.3ZHENGYMYUHQLIUSHetal.FormationandinstabilityofaerobicgranulesunderhighorganicloadingconditionsJ.Chemosphere200663101791-1800.4.J.2008293696-702.WANGShan-quanKONGYun-huaYUANYuanetal.FilamentousovergrowthinaerobicgranulesJ.EnvironmentalScience2008293696-702.inChinese5EIKELBOOMDH.FilamentousorganismsobservedinactivatedsludgeJ.WaterResearch197594365-388.6TAYJHLIUQSLIUY.Characteristicsofaerobicgranulesgrownonglucoseandacetateinsequentialaerobicsludge13012011blanketreactorsJ.EnvironmentalTechnology2002238931-936.7.SBARD.2004.WANGFang.CultivationandcharacteristicsofaerobicgranularsludgeinsequencingbatchairliftreactorD.DalianSchoolofEnvironmentalScienceandTechnologyDalianUniversityofTechnology2004.inChinese8CHUDOBAJ.Controlofactivatedsludgefilamentousbulking—ⅥFormulationofbasicprinciplesJ.WaterResearch19851981017-1022.9deKREUKMKvanLOOSDRECHTMCM.SelectionofslowgrowingorganismsasameansforimprovingaerobicgranularsludgestabilityJ.WaterScienceandTechnology20044911-129-17.10LIUYYANGSFTAYJH.ElementalcompositionsandcharacteristicsofaerobicgranulescultivatedatdifferentsubstrateN/CratiosJ.AppliedMicrobiologyandBiotechnology2003615-6556-561.TheEffectsofSubstrateTypeandConcentrationonFilamentousGrowthinAerobicGranularSludgeSBRGAOJing-fengSUKaiZHANGQianCHENRan-niPENGYong-zhenCollegeofEnvironmentalandEnergyEngineeringBeijingUniversityofTechnologyBeijing100124ChinaAbstractTheeffectsofsubstratetypeandconcentrationoncultivationandstabilityofaerobicgranularsludgeareinvestigated.Duringstart-upphasesucroseisusedasthesolesubstratecompactaerobicgranularsludgeformin10daysunderinfluentCODconcentrationof600-900mg/Lthemeandiameterofthegranuleis1.15±0.14mmSVIis90mL/g.HoweverwhentheinfluentCODconcentrationisincreasedfrom900to1200mg/LoutgrowthoffilamentousbacteriainaerobicgranularsludgeoccurwhenSVIisincreasedto175mL/gandlargeamountsofaerobicgranularsludgearewashedoutofthereactor.Inordertocontrolthefilamentousgrowthinaerobicgranularsludgesucrose+peptone1∶1areusedasthesubstrateunderinfluentCODconcentrationof600mg/Lduring34daysthefilamentousbacteriagraduallydisappearthefilamentousgrowthaerobicgranularsludgetransitstostablesmoothaerobicgranularsludgeagain.Whenthe