电化学强化脱氮中反硝化菌对活性炭纤维电极电化学反应影响初步研究应迪文

:,,1981,,-。电化学强化脱氮中反硝化菌对活性炭纤维电极电化学反应影响初步研究应迪文　贾金平　张乐华(,200240)　　,。,,,,。　　　　　Influenceofdenitrifyingbacteriaontheelectrochemistryreactionofactivatedcarbonfibreinbio-electrochemicalsystemYingDiwen,JiaJinping,ZhangLehua.(SchoolofEnvironmentScienceandEngineering,ShanghaiJiaotongU-niversity,Shanghai200240)Abstract:　Abio-electrochemicalreactorconsistedofactivatedcarbonfibreelectrode,immobilizedofdenitrifyingbacteria,ascathodewasstudied.Thecatholicpolarizationcurveandnitrateconsumptionratewereusedtoconfirmtherateofelectrochemistryreactionandtheactivityofdenitrifyingbacteria,respectively.Itwasshownthatdenitrificationwasaffectivelytotheelectrochemistryreaction,andthemodifiedextrapolatedexchangecurrentdensityfromhighoverpotentialswasrelativelytotheactivityofdenitrifyingbacteria.Keywords:　Bio-film　Denitrification　Electricchemistry　Catholicpolarization　　,[1]。,30mg/L,[2]。,,、。A2/O,。、[3],。、,。,/[4],[5,6],。、,[7]。。,,,,。1　1.1　反硝化菌富集及分离1　　　。,1(a)。2L,25℃。:C6H12O65.0g/L,KH2PO4K2HPO40.5g/L,NaNO31.0g/L,CaCO30.2g/L,MgSO4,pH7.2。5～7d,25mg/(Lh),3500r/min。501　DOI:牨牥牨牭牴牳牭牤jcnki牨牥牥牨牫牳牰牭牪牥牥牭牥牱牥牥牱1.2　电极制备。:1.25cm×1.00cm。(PVA,1750±50),[8]PVA。1.25cm×1.00cm,PVA。1.3　固定反硝化菌活性炭纤维电极电化学驯化培养及阴极极化扫描1(b),500mL。(WYJ)(DB2-DA3),、,,(25±1)℃。:KH2PO45mg/L、K2HPO43mg/LNaNO330mg/L,pH7.1～7.2。。,(1.4)。,0.005mA/cm2(6.25×10-3mA,1.2V)、0.025mA/cm2(2.5V)、0.050mA/cm2(3.1V),,。0.050mA/cm2,2～3℃。PVA,。1.4　实验测定方法,[9](PCSUV-2102,UNICO)。,(AUTOLABpotentiostatgalvanos-tat,modelPGSTAT30)。,,,3cm。5min。。-1.5～0V,0V,1.25mV/s。,,。2　2.1　反硝化菌对活性炭纤维电极电化学反应速度的影响[7](HydrogenEvolutionReaction,HER);[10],HER。Volmer:H2O+e←→H(a)+OH-(1)Heyrovsky:H2O+H(a)+e←→H2(g)+OH-(2)Tafel:2H(a)←→H2(g)(3):H2O+2e=H2+2OH-(4)HER,[11]HER,η0,j(αHαV)=2j0V(1-θe)exp[-(1-αv]fη;(5)j(αHαV)=2j0VmHθeexp[-(1-αv]fη](6):j,A/cm2;j0V,A/cm2;η,V;θe;αVVolmer;αHHeyrovsky;f,F/RT;mHVeH/VeV。Tafel,log(j(η))=logjhexp+ηb(7)jhext(αHαV)=2jV0(1-θe),bh(αHαV)=2.3026f(1-αV)(8)jexth(αHαV=2j0vmHθe,bh(αHαV)=2.3026f(1-αH)(9):jexth,A/cm2;bhTafel。jexthHER,HER。。,,。PVA0.05mA/cm22。,(-η0.3V),。Tafel,。25℃,0.05mA/cm2,5.41×10-4A/cm2,0.232mg/h;,2.73×10-4A/cm2、0.005mg/h,49.5%、97.8%。2～4℃,2.28×10-4A/cm2、0.011mg/h,,1.84×10-4A/cm2、0.002502　　27　7　200510mg/h;57.8%、95.0%。2　　　:1.25℃,0.050mA/cm2,;2.2～4℃,0.050mA/cm2,;3.25℃,0.050mA/cm2,;4.2～4℃,0.050mA/cm2,。(■),(○)logjhext,(…)Tafel,(j),A/cm2。,25℃,0.050mA/cm2PVA,,,。,NO-2、NO、N2O[12]。,,,。,。PVA,,49.5%;,。,,97.8%,,。2～4℃。。,2～4℃25℃,,41.4%,94%。,,,。HER,,,HER。,,。3。,HER,,。3　4　(I)—(II)—　　:1-;2-0.005mA/cm2;3-0.025mA/cm2;4-0.050mA/cm2。(■),(○)logjexth,(…)Tafel,(j),A/cm。2.2　反硝化菌反硝化能力对活性炭纤维电极电化学反应速度影响的关联分析4,0mA/cm20.005、0.025、0.050mA/cm2,503　,3.53×10-4、3.97×10-4、4.62×10-4、5.41×10-4A/cm2,0.021、0.091、0.139、0.232mg/h;,,,,2.1。PVA3.58×10-4、3.00×10-4、3.02×10-4、2.73×10-4A/cm2。,,。、,。,0.0089、0.0061、0.0074、0.0053mg/h。,。,,。PVA,HER,HER,。,,。,,=-PVA5　1-;2-0.005mA/cm2;3-0.025mA/cm2,4-0.050mA/cm-25,0.993。,,。,、,,。3　　,,,;,;,,。1　DahabMF,LeeYW.Nitrateremovalfromwatersuppliesusingbiologicaldenitrification.JWPCF,1988,60:1670～16772　　,,,.--.,2001,21(3):257～2623　　,,..,2003,24(4):109～1134　ZhangLH,JiaJP,ZhuYC,etal.Electrochemicallyimprovedbio-degradationofmunicipalsewage.BiochemicalEngineeringJournal,2005,22(3):239～2445　,..,1996,15(6):25～276　SakakibaraY,ArakiK,TankakaT,etal.Denitrificationandneu-tralizationwithanelectrochemicalandbiologicalreactor.Wat.Sci.Tech.,1994,30(6):151～1557　.-:[].:,2005.92～998　,,,..,2001,21:207～2119　..:,199710　FrankenthalRP,MilnerPC.Hydrogenevolutionkineticsonahigh-carbonsteelandontininseawater.Corrsion,1986,42:51～5311　GenneroMR,ChialvoAC.Analysisofanunusualbehaviorofthehydrogenevolutionreaction:TwoTafelianregionswithe-qualslope.InternationalJournalofHydrogenEnergy,2002,27:871～87712　BruceER,PerryLM.Environmentalbiotechnology.PrinciplesandApplications,McCarty-HillEducation(Asia)Co.,2001.498～503责任编辑:闵　怀　(:2005-07-13)504　　27　7　200510