电极生物膜脱氮工艺中反硝化菌相分析鲍立宁

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:2004-05-13:(50478065)、(zd0003)。:(1975-),,,,。125()Vol.12No.52004JournalofAnhuiInstituteofArchitecture&Industry2004鲍立宁1,2,洪桂云1,黄显怀1,3(1., 230022;2., 230036;3., 230029) :,40,,(Pseudomonas),(Acinetobacter),(Enterobacteriaceae)3,、,82.5%。24,,18,75%;8,33.3%。16,,12,75%;8,50%。:;;;:X132   :A   :1006-4540(2004)05-001-04  [1],[2]。,。,,[3,4]。,,,H2CO2。OH-,pH,,。,,。,,,。1 1.1 ,。1.2 1.2.1 1。1.2.2 4/1(pH7.2~7.6): 2g MgSO4·7H2O 0.2g K2HPO4 0.5g NaAc 4g  20g  1000ml1 ,(pH7.2~7.6): 2g MgSO4·7H2O 0.2g K2HPO4 0.5g  20g  1000ml1.2.3 ,、。:、,,,。,20g,20%NaOH200ml,。,,CO2∶H2=1∶3。1.3 、,、12[5,6]。,[7,8]。2 2.1 ,,。、,。,,,。,CO2、H2。,,,,,。2.2 (1、2)1        MR VP12A-、、、,,,-++d+---+*-,12B-12A-++d+---+*+12D-、12A-++d+---+*+12E-12A-+-++---+*+13A-、12A-+-d+---+*+13B-、,,,-+-d+---+*+,13C-、12A-++d+---+*+16A-、,,,-+--+---+*-,2()            12  1       MR VP16B-、、16A-+--+---+--16C-、、12A-++d----+*-16D-、12A-+--+---+*-17B-,12A-+-d----+*+17C-、12A-+-d+---+*-17D-、12A-++-+---++-19B-、,,-++-+-+-++-,19C-、,,-++-+-+-+++19D-、19C-++d+-+-+*+22A-、12A-+-++--++*+22B-、,,,-+-++--++*-,25A-、,-++++--++*+25B-25A25A-++++--++*-25C-25A25A-++++-++*+25D-25A25A-++++---+*-25E-25A25A-++++---+*-1-,,,-+-d+--++++2-、1-+-d----+++3-1-+-d+---+++4-、,-+-d+---++-11-,,,-+-++---+++12-、、,,,-+-++---+*+13-、、,-++-+---+--14-,-+-++---+*+15-、、,,,-+--+---++-17-,-+--+-+-+++18-、、、,,,-+--+---+++19-、、,,-++++---+++20-、、,,---++---++-21-15---++---+++22-、、,-+-++-+-+*+23-,-++++-+-+*+  :d;*,,2 (%)(%)(%)833.3520.814.212E*,13A,13B,16A,16B,16D,17B,17C1458.31145.8520.812A,12B,12D,13C,16C,17D,19B,19C,19D,25A*,25B*,25C*,25D*,25E*,28.328.328.322A*,22B*241001875833.3850850531.22,3,4,11*,12*,14*,20*,21*318.7212.5212.513,19*,23*531.3212.516.31,15,17,18,22*161001275850  :“*”。35       ,,:3  (1)403,(Pseudomonas),(Acinetobac-ter),(Enterobacteriaceae)。,33,42.5%42%。(2)24(12A.12B.12D.12E.13A.13B.13C.16A.16B.16C.16D.17B.17C.17D.19B.19C.19D.22A.22B.25A.25B.25C.25D.25E),,58.3%;18,75%;8,33.3%。(3)16(1.2.3.4.11.12.13.14.15.17.18.19.20.21.22.23),,50%;12,75%;8,50%。(4)24,9;16,。:,;,,。1 ,,..,2001,21(2):133~136.2 ,..,2002,31(1):19~21.3 ,,.——.,2001,21(3):257~260.4 KERRIL.CASTandJOSEPHR.V.FLORA,ANEVALUATIONOFTWOCATERIALSANDTHEIMPACTOFCOPPERONBIO-ELECTROCHEMICALDENITRIFICATION.Wat.Res.,1998,32(1):63~70.5 、..:,2002.145-157;251~266.6 ,.,,1997,16(2):16~19.7 R.E,.,N.E,..:.1984.8 、.,:,2001.66~378.ANALYSISOFDENITRIFYINGBACTERIAINBIOFILM-ELECTRODEPROCESSBAOLi-ning1,2,HONGGui-yun1,HUANGXian-huai1,3(1.Dept.ofEnvironmentalEngineering,AnhuiInstituteofArchitecture&Industry,Hefei,230022,China;2.SchoolofScience,AnhuiAgricultureUniversity,Hefei,230036,China;3.SchoolofMaterialsScienceandChemistry,UniversityofScienceandTechnologyofChina,Hefei,230029,China)Abstract:Thepreliminaryseparationforgroupofbacteriasurvivinginthebiofilm-electrodedenitrificationreactorhadbeeninvestigated.Theresultshowedthatthreegroupshadbeenidentifiedfrom40bacteriastrainsbyaseriesofmorphologicalobservationandphysiology-biochemistryexperiments.TheyarePseudomonas,AcinetobacterandEn-terobacteriaceae.ThedominantbacteriaamongthemwereEnterobacteriaceae&Pseudomonaswith82.5%.Eighteentypesofbacteriafordenitrificationwereinvolvedinthetwenty-fourgroupsseparatedintheheterotrophicreactorwith75.0%ofEnterobacteriaceaeasdominantbacteria,eighttypesarehighefficiencywith33.3%;Twelvetypesofbac-teriafordenitrificationwereinvolvedinthesixteengroupsseparatedintheautotrophicreactorwith75%ofPseu-domonasstrains,eighttypesarehighefficiencywith50%.Keywords:Biofilm-electrode;DenitrifyingBacteria;Separation;Identification4()            12

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