顶空固相微萃取气质联用技术分析5种荷花的挥发性成分徐双双

306Vol.30．No．620116ChineseJournalofAnalysisLabor[e-mail]-512211*121．2710182．250014-HS-SPME-GC-MS5。65μmPDMS/DVBSPME25℃。574、90%。54－－1－1－－3.1.0β-3。O657.63A1000-0720201106-054-03NelumbonuciferaGaertn．Nymphaeaceae7～8。、、、、、、、、、。。1。Yang2HPLC-DAD/MS15。HS-SPME-GC-MS5。11.17890N－5973NGC-MSD-AgilentGC-MSDNIST05、WILEY275Agilent。SPME65μmPDMS/DVB/SPME70μmCarbowax/DVB/SPME100μmPDMSSPME。、、、、5。1.21.2.1HP－5MS30m×0.25mm×0.50μm60℃1min3℃/min160℃8min8℃/min260℃8min—45—DOI:10.13595/j.cnki.issn1000-0720.2011.0161306Vol.30．No．620116ChineseJournalofAnalysisLaboratory2011－61.0mL/min280℃。1.2.2EI70eV200μA230℃280℃150℃m/z10～500amu。1.3GC-MS250℃2h。2g10mL65μmPDMS/DVBSPME25℃60minGC-MS250℃2.5min。1.4MSDChemStationNIST05WILEY275。22.1-345。K。365μmPDMS/DVBSPME70μmCarbowax/DVBSPME100μmPDMSSPME60℃25℃65μmPDMS/DVBSPME25℃。。2.21.21.31.4＞80%。574、、。59、D－、α－、、、、、14－、8－。5、4－－1－1－－3.1.0－2－246－10－2－84－－1－1－－3.1.01－－4－1－－3－－1－266－－1－－1－63－E－5－3.3.1Z－3－1－83－－15－25－2610－4－2－2.2.2Z－3－2－61－－23－7－1－－4.1.07－－β-1－71013－。55。5。334--1-3.1.015.874%15.713%3424.413%8.398%3324.314%10.049%3334.814%14.058%32β-21.926%16.092%。1、。1560.8%—55—306Vol.30．No．620116ChineseJournalofAnalysisLaboratory2011－61860.1%616.1%。1Fig.1VolatilecomponentsandrelativecontentsindifferentvarietiesofNelumbonucifera2.3。、、、、。。、、α－1－。。。、、。1．20103021452YangRZWeiXLGaoFFetal．JChromatogrA2009121611063．20022032024．20011943145．199994315AnalysisofvolatilecomponentsfromthepetalsoffivevarietiesofnelumbonuciferabyHS-SPME-GC-MSXUShuang-shuang12ZHAOXian-en2LIUYu-qin1DUJin-hua1andWANGXiao*211．CollegeofFoodScienceandEngineeringShandongAgriculturalUniversityTaian2710182．ShandongAnalysisandTestCenterShandongAcademyofSciencesKeyLaboratoryforAppliedTechnologyofSophisticatedAnalyticalInstrumentofShandongProvinceJinan250014FenxiShiyanshi201130654～56AbstractVolatilecomponentsfromthepetalsofNelumbonuciferainfivevarieties“Honghuhonglian”“Hongwanwan”“Hongmudan”“Baiwanwan”and“Huangmudan”wereanalyzedbyheadspacesolidphasemicroextractionSPMEcombinedwithGC-MS．TheeffectsofdifferentSPMEfibersandextracttemperatureontheextractionwereinvestigated．Theresultsdemonstratedthatselectionof65μmPDMS/DVBfibreandroomtemperatureof25℃inextractingthevolatilecomponentsfromthepetalsofNelumbonuciferaexhibitedgoodextractioneffect．Thecontentsofthevolatileconstituentsweredeterminedbypeakareanormalizationmethodatotalof74compoundswereidentifiedinfivevarietieswhichweremainlymadeupofalkanesalkenesandalcoholsandtheircontentsexceeded90%．ComparedwiththevolatileconstituentsoffivekindsofNelumbonuciferaitshowedthatthecompositionofthevolatilecomponentsinthefivevarietieswasdifferentbutthemaincomponentsweresimilar．Amongthemthehighestcontentcomponentsof“Honghuhonglian”and“Huangmudan”were4-methylene－1－1-methylethyl-Bicyclo3.1.0hexaneandβ-phellandrenerespectivelywhilethehighestcontentcomponentofotherthreevarietieswaspentadecane．ThismethodisrapidandaccurateandprovidestheoreticalfoundationforfurtherdevelopmentanduseoftheNelumbonuciferaresources．KeywordsNelumbonuciferaVolatilecomponentsHeadspacesolidphasemicroextractionHS-SPMEGC-MS—65—