动胶菌HP3对芳香化合物的降解及取代基效应分析黄丽萍

　　:1000-1735(2003)03-0286-04HP3黄丽萍,　周集体,　腾丽曼(,　116024)　:HP3.,,1,4--2-、1--2-、9,10-、1,4,5,8-,、、、,、,2--3--5-2,3--5-...:溴胺酸;生物降解;动胶菌;芳香化合物;取代基:X172　　　:A,、[1],,..HP3(1--4--2-)[2,3].,、、、VmKm,,、,,.1　实验材料与方法1.1　Na2HPO4:1.3g/L,KH2PO4:2.0g/L,FeCl3:1mL/L(0.25g/L).::2.0g/L,(NH4)2SO4:1.0g/L,Na2HPO4:1.3g/L,KH2PO4:2.0g/L,FeCl3:1mL/L(0.25g/L).1.2　.1.3　HP34℃,10000rad/min,(TOC).TOCTOC—5000.150mL/min,CV5%,(TC26μL,IC33μL),680℃,IC.SO2-4EDTA[4].1.4　4℃,10000rad/min,JascoUV—560—:2003-07-02:(199902):(1968-),,,,.(1956-),,,,.第26卷第3期2003年9月　　　　　辽宁师范大学学报(自然科学版)JournalofLiaoningNormalUniversity(NaturalScienceEdition)　　　　　Vol.26　No.3Sep.　2003,,.1.5　VmKm.(OD6600.65),4.5mL(、、)(KH2PO4-Na2HPO4,pH=7.0),0.5mL,30℃,100rad/min0.5h,,,VmKm.2　实验结果与讨论2.1　HP3(1),14mM()6.8mM,.SO2-4,1--2-1,4--2-SO2-4,SO2-4.,1--2-1,4--2-,[5,6].TOC,1,4--2-1--2-.,9,10-1,4,5,8-[7],,9,10-1,4,5,8-,10%,.TOC(2),HP39,10-1,4,5,8-,9,10-1,4,5,8-.,,,9,10-1,4,5,8-.图2　HP3菌降解9,10-蒽醌及1,4,5,8-四羟基蒽醌的总有机碳变化图1　HP3菌降解蒽醌磺酸类化合物的总有机碳变化2.2　HP3、、、、.3,HP3、、.、,,[8,9].TOC,≈.1V-1S,xVm,KmVmKm,.,Vm,KmKm,VmKmVmKm.,,第3期黄丽萍等:　动胶菌HP3对芳香化合物的降解及取代基效应分析287　　VmVmKm,,.VmVmKm,,.图3　HP3菌降解苯系化合物过程中的总有机碳变化　附表　HP3细胞总酶液对相应底物酶促反应的Vm,Km及VmKm值比较底　　　　物参　数苯　胺苯　酚邻苯二酚邻苯二甲酸溴胺酸Vm(mmol/mg·h)0.33760.25570.38380.14500.07815Km(mmol/L)0.12210.16400.34060.52741.382VmKm(L/mg·h)2.7651.5591.1270.27490.056552.3　[10,11],、、.,,,().,1--2-、1,4--2-、1,4,5,8-9,10-,-NH2、-Br,、,,.,HP3,().,,1、41,,4-Br,1,4--2-1--2-.9,10-1,4,5,8-,,、9,10-1,4,5,8-..[12,13],.,HP3.HP3、、、,、,2--3--5-2,3--5-[9].,.:,,,;Brown[14],,,.,[11],,-OH、-NH2,、.,.,[15],.,.2--3--5-2,3--5-,,[16],.3　结论HP3,,1,4--2-、1--2-、9,10-、1,4,5,8-,、288　　辽宁师范大学学报(自然科学版)第26卷、、,、2--3--5-2,3--5-,、、.HP3,VmVmKm,,.:[1]　,.[J].,1998,6(4):22.[2]　,,,.HP3[J].,2000,20(5):557-561.[3]　HUANGLP,ZHOUJT,YANGFL,etal.Biodegradationof1-amino-4-bromoanthraquinone-2-sulfonatebyZoogloeaitzigohnHP3[A].HUXJ,POLY.ProceedingsoftheThirsAsiaPacificConferenceonSustainableEnergyandEnvironmentalTechnologies[C].Singapore:WorldScientif-icPublishingLtd,2000.203-207.[4]　.[M].3.:,1989.[5]　,,,.HP3[J].,2002,22(3):364-368.[6]　,,,.HP3[J].,2001,21(2):180-184.[7]　.(3)[M].:,1990.8.[8]　,.[J].,1991,10(6):12-17.[9]　,,,.H-[J].,2000,13(2):6-8.[10]　.--[M].:,1999.[11]　ZEVERJ,WASSERFALLENA,TIMMISKN.Microbialmineralizationofring-substitutedanilinesthroughanortho-cleavagepathway[J].ApplEnvironMicrobiol,1985,50:447-453.[12]　EATONDC.MineralizationofpolychlorinatedbiphenylsbyPhanerochaetechrysosporium:aligninolyticfungus[J].EnzymeMicrobTechnol,1985,7:194-196.[13]　PASZCZYNSKIA,HUYNHVB,CRAWFORDR.Comparisonofligninase-1andperoxidase-M2fromthewhite-rotfungusPhanerochaetechrysosporium[J].ArchBiochemBiophys,1986,244:750-765.[14]　BROWND,LABOUREURP.Thedegradationofdyestuffs:PartⅠ.Primarybiodegradationunderanaerobicconditions[J].Chemospere,1983,12(3):397-404.[15]　YICC,PAULHW,KUENSL,etal.Fateofbromineinpyrolysisofprintedcircuitboardwastes[J].Chemosphere,2000,40:383-387.[16]　,,.[J].,1993,12(3):161-172.EffectsonBiodegradationofAromaticCompoundsbyZoogloeaitzigohnHP3andAnalysisofSubstituteGroupsHUANGLi-ping,　ZHOUJi-ti,　TENGLi-man(SchoolofEnvironmentalandBiologicalScienceandTechnology,DalianUniversityofTechnology,Dalian116024,China)Abstract:ZoogloeaitzigohnHP3wasextractedfromthesoilcontaminated,whichcandegradeeffectivelynotonly1-amino-4-bromoanthraquinone-2-sulfonatebutalso1,4-dihydroxyanthraquinone-2-sulfonate,1-aminoanthraquinone-2-sulfonate,9,10-dihydroxyanthraquinoneand1,4,5,8-tetrahydroxyanthraquinone.Moreover,aniline,phenol,o-di-hydroxybenzeneando-phthalicacidcanbealsodegradedbyHP3,whereas2-amino-3-hydroxyl-5-bromobenzene-sulfonateand2,3-dihydroxybenzenesulfonatewhichwereproductsofABASdegradedbyZoogloeaitzigohnHP3,benzenesulfonateandp-amino-benzenesulfonatecannotbefurtherdegradedbyHP3.Anilinewasthemosteffective-lydegradedcompoundamongtheexperimentalbenzenecompounds.Electroniceffectsandspaceeffectsresultingfromeffectsofsubstitutegroupswerediscussedinthispaper.Keywords:1-amino-4-bromoanthraquinone-2-sulfonate;biodegradation;Zoogloeaitzigohn;aromaticcompounds;substitutegroup第3期黄丽萍等:　动胶菌HP3对芳香化合物的降解及取代基效应分析289