短程反硝化除磷工艺的可行性研究张少辉

31　24200912武　汉　理　工　大　学　学　报JOURNALOFWUHANUNIVERSITYOFTECHNOLOGYVol.31　No.24　Dec.2009DOI:10.3963/j.issn.1671-4431.2009.24.009张少辉,黄　毅(,430070)　:　采用SBR反应器,以A/O工艺好氧段污泥为种泥,研究了以亚硝酸盐为电子受体的反硝化除磷菌的筛选与富集,并对在厌氧/缺氧的条件下利用亚硝酸盐为电子受体的反硝化除磷工艺的可行性及亚硝酸盐消耗量与磷酸盐去除量间关系进行了考察,结果表明:采用厌氧/沉淀排水/缺氧/沉淀排水的运行方式有利于短程反硝化聚磷菌的筛选与富集;以亚硝酸盐作为电子受体的短程反硝化除磷是完全可以实现的,31d完成了短程反硝化除磷系统的启动,COD去除率达69.6%,PO3-4-P去除率达79.2%,NO-2-N去除率达98.9%;在一定的碳源浓度和磷酸盐充足的条件下,缺氧段消耗单位亚硝酸盐的吸磷量为1.39mgP/mgN。:　亚硝酸盐;　短程反硝化除磷;　反硝化聚磷菌:　X703:　A:1671-4431(2009)24-0034-04FeasibilityofShort-cutDenitrifyingPhosphorusRemovalProcessZHANGShao-hui,HUANGYi(SchoolofCivilEngineeringandArchitecture,WuhanUniversityofTechnology,Wuhan430070,China)Abstract:　Theselectionandenrichmentofdenitrifyingphosphorusremovalbacteriausingnitriteaselectronicacceptorwereresearchedinsequencingbatchreactor(SBR)withtheanoxicsludgeofA/Oprocessasinoculum.Thefeasibilityofdenitrifyingphosphorusremoval(DPR)processwasexploredunderanaerobic/anoxicconditionusingnitriteaselectronacceptor,andtheamountrelationshipbetweennitriteconsumptionandphosphorusremovalwasinspected.Theresultsshowthat,theoperationmode,i.e.anaerobic/sedimentationanddisplacement/anoxic/sedimentationanddisplacementisbeneficialtothescreeningandenrichmentofdenitrifyingphosphorusremovalbacteriausingnitriteaselectronicacceptor.Theshort-cutdenitrifyingphospho-rusremovalsystemusingnitriteaselectronacceptorissuccessfullystartedupin31d,theremovalratesofCOD,PO3-4-PandNO-2-Ncanreach69.6%,79.2%and98.9%,respectively.Withthesamecarbonsourceconcentrationandsufficientphos-phate,theratioofphosphorusuptaketonitriteconsumptionis1.39mgP/mgNunderanoxiccondition.Keywords:　nitrite;　short-cutdenitrificationphosphorusremoval;　denitrifyingphosphorusremovalbacteria:2009-07-25.:(1972-),,,.E-mail:[e-mail],,:(DPB),DPB。,Tsuneda[1](nir)。Meihold[2]SBR8mg/L,。[3],30mg/L,。2003Hu[4]O2、NO-3、NO-23,,。,2-。SBR,/,。1　　　　1)　SBR,,,3.43L。,。,。1。2)　,NaAcCOD,KH2PO4PO3-4,NaNO2NO-2,NH4ClNH+4,CaCl210mg/L,MgSO4·7H2O90mg/L,1mL/L,1。　　:FeCl31.5g/L,H3BO30.15g/L,CoCl2·7H2O0.15g/L,CuSO4·5H2O0.03g/L,MnCl2·4H2O0.06g/L,Na2MoO4·2H2O0.06g/L,ZnSO4·7H2O0.12g/L,KI0.18g/L,EDTA10g/L。3)　(1)　A/O1　/(mg·L-1)CODPO3-4-PNO-2-NNH+4-N150～3606～165～3230150～3506～165～3230280104～2530,,3000～3500mg/L。25～26℃///,。2,3h,1h,4.5h,1h,18d,,PO3-4NO-2,PO3-4-PNO-2-N。COD50mg/L,,。(2)　,8.5h,2,3h,4.5h,1h,1.71L,0.3L。pH7.2±0.2,18d。(3)　1.2L,4,1000mL,300mL,MLSS(3000±200)mg/L,PO3-4-P10mg/L,COD280mg/L,pH7.2±0.2。4:4mg、10mg、15mg、25mgNO-2-N/L,3h,NaNO2,PO3-4-PNO-2-N。4)　COD[5];PO3-4-P;NO-2-NN-(1-)-;MLSS;pH。2　2.1　,,PO3-4-PNO-2-N,3531　24　　　　　　　　　　,　:　　　　　　　　　。COD、PO3-4-P、NO-2-N2。2,,,15d,COD43.48mg/L84.01mg/L,7.80mg/L11.79mg/L,COD,31d48d,COD107.22mg/L,21.25mg/L,58d,COD135.88mg/L,23.66mg/L,,。,COD0.1790.140,,PHB,,,,19d,0.2,0.06～0.4[6],。3,27d,NO-2-N5.02mg/L10.05mg/L,NO-2-N1mg/L,90%,PO3-4-P64.4%93.6%,NO-2-N。NO-2-N15mg/L,NO-2-N31d4.04mg/L,8dNO-2-N0.35mg/L,PO3-4-P76.5%～99.2%。NO-2-N18.66mg/L32.50mg/L,NO-2-N1.67mg/L4.47mg/L,58d1mg/L,PO3-4-P12～16mg/L,PO3-4-P80%～90%。,,NO-2-N,,,,,NO-2-N。,,,NO-2-N。///,COD185mg/L361mg/L,COD30mg/L,COD,,,。58d,COD75.4%,PO3-4-P81.8%,NO-2-N97.4%,,,。2.2　,//,,32mg/L,COD4。4,NO-2-N4.87mg/L32.27mg/L,NO-2-N,31d,NO-2-N0.36mg/L,98.9%,。5d,PO3-4-P6mg/L,20%～30%,NO-2-N,,,,27d,PO3-4-P15mg/L,36　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　200912PO3-4-P98.3%。31d,PO3-4-P16mg/L,79.2%,70%,。,PO3-4-P,。COD14d81.8%60.5%,19dCOD,29d82.7%,70%～80%,,,,,COD,,,COD。31d,COD69.6%,PO3-4-P79.2%,NO-2-N98.9%,,,,。2.3　,,,NO-2-NPO3-4-P,5。[7]:0.52mgP/mgN,:1.39mgP/mgN,。。NO-2,NO-3,PHB,,NO-2NO-3。SRT、pH、,,。3　　a.///。58d,COD75.4%,PO3-4-P81.8%,NO-2-N97.4%,,,。b.,SBR31d,COD69.6%,PO3-4-P79.2%,NO-2-N98.9%。c.,,1.39mgP/mgN。[1]　TsunedaS,DaidouT,AhnJ,etal.CharacterizationofDenitrifyingPhosphate-accumulatingOrganismsCultivatedUnderDif-ferentElectronAcceptorConditionsUsingPolymeraseChainReaction-denaturingGradientGelElectrophoresisAssay[J].WatRes,2002,36(2):403-412.[2]　MeinholdJ,ArnoldE,IsaacsS.EffectofNitriteonAnoxicPhosphateUptakeinBiologicalPhosphorusRemovalActivatedSludge[J].WaterRes,1999,33(8):1871-1883.[3]　　,　,,.[J].,2007,27(7):1141-1144.[4]　HuJY,OngSL,NgWJ,etal.ANewMethodforCharacterizingDenitrifyingPhosphorusRemovalBacteriabyUsingThreeDifferentTypesofElectronAcceptors[J].WatRes,2003,37(14):3463-3471.[5]　.[M].4.:,2002.[6]　HuZhi-rong,WentzelMC,EkamaGA.AnoxicGrowthofPhosphate-accumUlatingorganisms(PAOs)inBiologicalNutrientRemovalActivatedSludgeSystem[J].WatRes,2002,36(2):4927-4937.[7]　.[D].:,2005.3731　24　　　　　　　　　　,　: