短程硝化厌氧氨氧化一步法自养脱氮中试研究韩晓宇

/韩晓宇1，2，常江1，2，孟春霖1，2，张亮1，2，甘一萍1，2，张树军1，2(1．北京城市排水集团有限责任公司，北京100022;2．北京市污水资源化工程技术研究中心，北京100124)一步法自养脱氮工艺在高氨氮废水处理中具有运行能耗低、不需外加碳源等优点。利用总容积为50m3的SBＲ反应器处理高氨氮废水，成功实现了短程硝化/厌氧氨氧化一步法自养脱氮。反应器对不同氨氮浓度(350～4300mg/L)的废水均表现出良好的处理效果，对氨氮与总氮的平均去除率分别达到95%和90%以上。同时，还研究了反应器运行的主要影响因素、污泥粒径分布及微生物群落结构。结果表明，系统内形成了红色的厌氧氨氧化颗粒，且颗粒的比例随运行逐渐增加;而维持合理的溶解氧和氨氮浓度是实现高负荷脱氮的关键因素。厌氧氨氧化;短程硝化;SBＲ反应器;自养脱氮X703A1000－4602201419－0001－06D131100003913001Single-stageAutotrophicNitrogenＲemovalBasedonPartialNitrificationandAnammoxHANXiao-yu12CHANGJiang12MENGChun-lin12ZHANGLiang12GANYi-ping12ZHANGShu-jun121．BeijingDrainageGroupCo．Ltd．Beijing100022China2．BeijingEngineeringＲesearchCenterforWastewaterＲeuseBeijing100124ChinaAbstractThesingle-stageautotrophicnitrogenremovalprocessforhighammonianitrogenwastewatertreatmenthasseveraladvantagesincludinglessenergyconsumptionnoneedtoaddextracar-bonsourceandsoon．Thesingle-stageautotrophicnitrogenremovalbasedonpartialnitrificationandAn-ammoxwassuccessfullyachievedusingapilot-scalesequencingbatchreactorSBＲwithatotalvolumeof50m3totreathighammonianitrogenwastewater．Thereactorperformedwellintreatmentofallwastewaterwithvariousammonianitrogenconcentrationsof350to4300mg/L．Theaverageremovalratesofammonianitrogenandtotalnitrogenwereabove95%and90%respectively．Besidestheaf-fectingfactorssludgeparticlesizedistributionandmicrobialcommunitystructurewereinvestigated．ＲedAnammoxgranuleswereobservedinthereactorwithacontinuousincreaseduringtheoperation．Appro-priateconcentrationsofdissolvedoxygenandammonianitrogenwerekeyfactorstoachievehighloadni-trogenremoval．KeywordsAnammoxpartialnitrificationSBＲreactorautotrophicnitrogenremoval·1·第30卷第19期2014年10月中国给水排水CHINAWATEＲ＆WASTEWATEＲVol．30No．19Oct．2014、。、、、12。35℃、10～12dpH。50m3SBＲ/。。1试验材料和方法1.16000～8000mg/L30m3UASB0．5m312000mg/LSBＲ。5000mg/L。NH4HCO3NaHCO3。1.2SBＲ3．5m5．5m5mpH、DO、、、1。30～35℃。1SBＲFig．1Schematicdiagramofpilot-scaleSBＲ1.3SBＲ440min、5mg/L、60min、35min。SBＲ116d7d31。1SBＲTab．1Influentqualityandoperatingparametersofpilot-scaleSBＲ/dNH3－N/mg·L－1COD/mg·L－1TP/mg·L－1pHDO/mg·L－1TN/kg·m－3·d－11401558262～4123．96～7．387．80．25～0．30．292392453329～5165．37～9．467．80．3～0．50．54330364270～6555．49～9．967．80．2～0．250．281.4NH3－NNO－2－NN－1－－NO－3－NTN—COD5B－3CpHDOpH/Oxi340iMLSS3。2结果与讨论2.1SBＲ1500mg/L938～2006mg/L298%93%31．1106mg/L。90%。。·2·第30卷第19期中国给水排水．watergasheat．com2Fig．2ProfilesofnitrogenremovalinSBＲindifferentperiods2SBＲ1651～4372mg/LSBＲ99%91%。4372mg/LNO－2－N300mg/L85%。。45。3350mg/L309～411mg/L80～100mg/L99%97%11．311．4mg/L。SBＲ。3。0．07kg/m3·d30．2kg/m3·d0．4kg/m3·d。20．5kg/m3·d。DO。SBＲ0．3kg/m3·d。3SBＲFig．3AmmoniumandTNremovalrateinSBＲ2.2FADO2.2.1FAFA6。FASBＲFA80mg/LFA0．80．6kg/m3·dNO－2－NNO－2－N。SBＲFA94mg/LNO－2－NNO－2－N300mg/L。SBＲFA50mg/L0．450．4kg/m3·dNO－2－N。2.2.2DO4SBＲ。·3·．watergasheat．com韩晓宇，等:短程硝化/厌氧氨氧化一步法自养脱氮中试研究第30卷第19期4DOSBＲFig．4ProfilesofnitrogencompoundsunderdifferentDOconcentrations7DO8SBＲDO。DO0．2～0．3mg/L0．280．25kg/m3·dDO0．4～0．5mg/L0．660．6kg/m3·dDO0．7～0．8mg/L1．101．02kg/m3·d10mg/LNO－2－NDO1．1～1．2mg/L1．0kg/m3·dDO310mg/L。DO0．7～0．8mg/L。2.3SBＲ2。52692。2Tab．2Molecularbiologyanalysisofsuspendedsludgeandgranule%AOBANAMMOXNitrobacterNitrospira5231．980．561．390．415．3812．510．7106932．131．460．820．096．529．710．670．052AOBANAMMOXNOB0．5%～1．5%0．9%～1．8%ANAMMOXNOB。ANAMMOXFANOB。ANAMMOX10%。ANAMMOX。NOB1%·4·第30卷第19期中国给水排水．watergasheat．com。。5。5SBＲFig．5ParticlesizedistributioninSBＲindifferentperiods5150～60μm50～60360～410μm19．6∶1260～70μm6．6∶1390～995μm80～90480～550μm22．9∶1。。Ni91．0～1．3mm400μmNH+4－N、NO－2－NNH+4－NNO－2－N0．55mmSBＲ。3结论①SBＲ。350～4300mg/L95%90%。②FASBＲFA94mg/LFA。③0．2～0．8mg/LSBＲDODO1．1～1．2mg/LDO。④SBＲ70d4．8%25．6%10%AOB。1JettenMSMStrousMvandePas-SchoonenKTetal．TheanaerobicoxidationofammoniumJ．FEMSMicro-biolＲev1998225421－437．2AbmaWSchultzCEMulderJWetal．TheadvanceofanammoxJ．Water21200736236－37．3．J．201431167－84．4．(下转第10页)·5·．watergasheat．com韩晓宇，等:短程硝化/厌氧氨氧化一步法自养脱氮中试研究第30卷第19期3．D．2011．4．4M．2002．5ChenJJMcCartyDSlackDetal．FullscalestudiesofasimplifiedaeratedfilterBAFfororganicandanitro-genremovalJ．WaterSciTechnol200041451－4．6．J．200523211－13．7．J．2003293172－174．8．J．2006202178－181．9．J．2007232060－62．10．J．2003235655－657664．11．—J．20022834－8．12．J．200525170－74．13．A/OJ．200826356－58．1987－。[e-mail]櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆櫆2014－04－11(上接第5页)J．201244275－79．5．FAFNAJ．2009303809－814．6VadiveluVMKellerJYuanZG．Effectoffreeammo-niaandfreenitrousacidconcentrationontheanabolicandcatabolicprocessesofanenrichedNitrosomonascul-tureJ．BiotechnolBioeng2006955830－839．7StrousMGervenEKuenenJGetal．Effectsofaerobicandmicroaerobicconditionsonanaerobicammoniumoxi-dizinganammoxsludgeJ．ApplEnvironMicrobiol19976362446－2448．8SliekersAO．Completelyautotrophicnitrogenremovalo-vernitriteinonesinglereactorJ．WaterＲes200236102475－2482．9NiBJChenYPLiuSYetal．Modelingagranule-basedanaerobicammoniumoxidizingANAMMOXprocessJ．BiotechnolBioeng20091033490－499．1984－。[e-mail]2014－05－09·01·第30卷第19期中国给水排水．watergasheat．com