短程硝化厌氧氨氧化组合工艺深度处理垃圾渗滤液吴莉娜

29420164ＲesearchofEnvironmentalSciencesVol．29No．4Apr．2016．-J．2016294587-593．WULinaXUYingyingSHIXiaoetal．AdvancedtreatmentoflandfillleachatebycombinedprocessofpartialnitrificationandanaerobicammoniumoxidationJ．ＲesearchofEnvironmentalSciences2016294587-[e-mail]．*[e-mail]-1222314*11．1500902．1026173．1000124．100124、UASB-AO-ANAMMOXＲ-ANAMMOX1∶10ρCODCr、ρNH4+-NρTN750±30290±10300±10mgL90d．CODCr、NH4+-NTN88%±1%、95%±1%91%±1%67±515±235±5mgLGB16889—2008《》．AOρFAFA0.21～1.38mgLNOBAOBNO2－-N70%～96%ANAMMOXＲANAMMOXNH4+-NNO2－-N．-X7031001-6929201604-0587-07ADOI10.13198j．issn．1001-6929．2016．04．17AdvancedTreatmentofLandfillLeachatebyCombinedProcessofPartialNitrificationandAnaerobicAmmoniumOxidationWULina12XUYingying2SHIXiao2YANGTianxue3PENGYongzhen14*ZHANGJie11．StateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentSchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnologyHarbin150090China2．BeijingInstituteofPetrochemicalTechnologyBeijing102617China3．LaboratoryofWaterSystemsEngineeringChineseＲesearchAcademyofEnvironmentalSciencesBeijing100012China4．KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentＲecoveryEngineeringBeijingUniversityofTechnologyBeijing100124ChinaAbstractInthisstudyanin-linesystemwithacombinedupflowanaerobicsludgebedUASB-anaerobicaerobicAO-anaerobicammoniaoxidationreactorANAMMOXＲwasusedfortheadvancedtreatmentofamixtureofmunicipallandfillleachateanddomesticwastewater．Partialnitrification-anaerobicammoniaoxidationwasemployedinthesystemtoachieveadvancednitrogenremoval．Thesystemsolvestheproblemsassociatedwithbiologicaltreatmentoflandfillleachatesuchashightoxicityofleachatetomicroorganismsunsatisfactoryorganicandnitrogenremovalandhighcostofadditionalcarbonsourcesupplemented．Theexperimentwasoperatedfor90dayswithaninfluentofa1∶10mixturevolumeratioofrawleachateanddomesticsewage．ThecorrespondingconcentrationsofρCODCrρNH4+-NandρTNwere750±30290±10and300±10mgLrespectively．TheresultsshowedthatthefinaleffluentsofρCODCrρNH4+-NandρTNwere67±515±2and35±5mgLwithremovalratesof88%±1%95%±1%and91%±1%respectively．Theeffluentqualitycompliedwiththedischargerequirementsofthe29StandardforPollutionControlontheLandfillSitesofMunicipalSolidWasteGB16889-2008．InadditionwhenthefreeammoniaFAconcentrationwas0.21-1.38mgLFAcouldinhibitnitrite-oxidizingbacteriaNOBbutnotammonia-oxidizingbacteriaAOBAOBwasthedominantbacteriaintheAOreactor．Moreoverpartialnitrificationwith70%-96%nitritenitrogenaccumulationratewasachievedintheAOreactorwhichwasfollowedbyANAMMOXＲtocompletelyremoveresidualammoniaandnitrite．KeywordslandfillleachatepartialnitrificationanammoxUASBAOreactor、NH4+-N．1-3ρNH4+-N4．GB16889—2008《》TN5．6-10．〔UASBup-flowanaerobicsludgeblanket〕11〔SBＲsequencingbatchreactor、、、12-15〕NH4+-NNOx－-NTN16-17．NOx－-N．40%、25%30%18-20．ANAMMOXanaerobicammoniumoxidation．ANAMMOXANAMMOXNO2－-NNH4+-NN212.ANAMMOX、．ANAMMOXNO2－-N．2223FA、pHρDO-ANAMMOXCN〔ρCODCrρTN〕．24．UASB-AO-ANAMMOXＲ-ANAMMOXNH4+-NTN、．11.1UASB+AO+ANAMMOXＲ1．-ANAMMOXNH4+-NTN．UASBNOx－-N．AO．UASBAOAOANAMMOXNH4+-NNO2－-N．AOANAMMOXＲANAMMOXＲANAMMOXNH4+-NNO2－-NANAMMOXＲANAMMOXNO3－-NTN．UASBANAMMOXＲ8.254.25L．AO15.00L101．1∶101.1.290d4.50LdUASBANAMMOXＲHＲT1.830.94dAOHＲT3.33d．8854-1UASB-AO-ANAMMOXＲFig.1FlowdiagramoftheUASB-AO-ANAMMOXＲsystem1Table1CharacteristicsofleachateρmgLCODCrNH4+-NTPNOx－-NTNCNpH5500±5002900±10012.00±3.0010±53000±1001.8～2.08.00±0.50340±4035±55.00±0.9043±3750±30290±105.63±1.015±1300±102.5～2.67.09±0.20UASB-AO-ANAMMOXＲ1a．UASB100%AO．UASBANAMMOXＲ35℃AO20～22℃ρDO0.30～1.00mgLρMLSS3000～4500mgL．raw、u1eUASB、A1AO、O2～O10AO、s1、u2iANAMMOXＲu2eANAMMOXＲ．1.3ρCODCr、ρNH4+-N、ρNO2－-NρNO3－-N25ρTNTNTOCMultiNC3000ρDOWTWDO330iWTWpH、pHWTWpH340iWTW．PCＲANAMMOX26ABI7300Ｒeal-TimePCＲSystemAppliedBiosystemsCAUSAPCＲSYBＲGＲEEN．HSBeta396FHSBeta742ＲhzsB27-28AＲGGHTGGGGHAGYTGGAAGGTYCCHACＲTCATGVGTCTG①95℃3min②95℃、30s59℃、30s72℃、30s4028-29．20μLSYBＲPremixExTaqTakara10μLＲOX50Takara0.4μL10mmolL1μL10DNA2μL20μL．GeneJetPlasmidMiniprepKitFermentasMBILithuaniaANAMMOXhzsB．NanoDrop2000ThermoFisherScientificWilmingtonDEUSADNAhzsB10．798529．3．PCＲ7300SDSSystemANAMMOX．90%～110%0.98DNA．22.1CN2.5～2.6.NO3－-NCN4.0NO2－-NCN＞2.530．31ANAMMOXρTN．32．2rawρCODCr740mgLu1e200mgLUASBρCODCr200mgLAOρCODCr90±10mgL．ANAMMOXρCODCr120mgLANAMMOX33．UASBρCODCr740mgL33UASBANAMMOX1.35×108g－1．AOANAMMOXＲρCODCrAOANAMMOXANAMMOX2ANAMMOX109g－1．ρCODCrρNOx－-NρNH4+-N2、4NOx－-N．ANAMMOXＲ．2ANAMMOXＲ、ρCODCrANAMMOXＲ．ANAMMOXＲANAMMOXNH4+-NNO2－-NANAMMOX．34———CANONcompletelyautotrophicammoniumremovalovernitriteANAMMOX108g－1ANAMMOX．3ANAMMOX5.43×109g－1ANAMMOX．35ρCODCr120mgLANAMMOX．ANAMMOXANAMMOXＲρNH4+-N、ρNO2－-NρNO3－-N15、155mgLρTN34mgL．2UASB-AO-ANAMMOXＲρCODCrFig.2VariationofρCODCrintheUASB-AO-ANAMMOXＲsystem1—UASB2—O13—O54—O105—ANAMMOXＲ．3ANAMMOXFig.3Analysisaboutvariationofthecopynumbersofgeneofanammoxbacteriainthesystem2.2ρNH4+-N290mgLUASBρNH4+-N106mgL．UASBNOx－-N．AO．ρDO1mgL．0954-4AOO1ρNH4+-N58mgLO2～O10ρNH4+-N．AOO10ρNH4+-N30mgLρNO2－-NρNO3－-N351mgLNO2－-N96%AON