©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(723100),:,,,;,,1972ChakrabartySAL,,,,,,,,1,,,,,,,22.12.1.12.1.2()2.1.3:,,95%,2.1.4:()(,)2.22.2.1:,10g,3cm5cm10g,2.2.2:1g,10mL(,),10-6gmL-12.2.3:,2.0gL-11.0mL(3,),,30,150rpm(20min,),2d3d,1.0mL3.0gL-1,,1.0mL4.0gL-12d3d(3),2.2.4:,,5.0gL-10.5mL,,,30,2d3d,7.0gL-1,9.0gL-1,32d3d3,2.2.5:,,33.13.1.1,1(,):1,,,,G++++,,G+/G-+3.1.2,(G+/G-,),,,,,;,20041945©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(NH4)2SO4AgarH2OMedium0.5/1.0/2.53.03.011.01000Medium5.0/7.0/9.03.03.012.020.01000:pH7.0,121.330min,,NaOHpH7.0,50mL,20mL;500mL;,,,121.330min,3,31,..,1995.6(3):3.2,,..,1995.12(6):3.3,...:,19992.4,..,2001.6(3):41.]?(064100),?,(P1)(P2),;,,,45s,,10min,,,?,,,(8),,,,,,,,,,]20041946