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3612201612ActaScientiaeCircumstantiaeVol．36No．12Dec．2016No．50978001“”No．2012ZX07102002SupportedbytheNationalNaturalScienceFoundationofChinaNo．50978001andtheMajorNationalProjectsonWaterPollutionControlandTreatmentNo．2012ZX071020021990—E-mailwuhaoen03@163．com*E-mailwzwu@pku．edu．cnBiographyWUHaoen1990—maleE-mailwuhaoen03@163．com!CorrespondingauthorE-mailwzwu@pku．edu．cnDOI10．13671/j．hjkxxb．2016．0308．2016．J．36124392-4399WuHEWeiCJWuWZ．2016．Studyonnitrogenremovalperformanceandmechanismofexternalcarbonsourceaddedtwo-stagesmulti-soil-layeringsystemsforlowpollutantloadingwastewatertreatmentJ．ActaScientiaeCircumstantiae36124392-4399，，*1008712015-12-262016-07-272016-07-29“+”multi-soil-layeringsystemMSLMSL．MSL1MSL2PHBVGC-4．10、、．MSL2MSL1．1．0m3·m－2·d－1．19℃＜15℃MSL1NH+4-N、TN91%、62%81%、45%MSL2NH+4-N、TN88%、72%80%、55%MSL2MSL1．2TN20%．MSLCOD．MSL、、MSL2MSL1amoAnirS、nirKMSL．0253-2468201612-4392-08X703AStudyonnitrogenremovalperformanceandmechanismofexternalcarbonsourceaddedtwo-stagesmulti-soil-layeringsystemsforlowpollutantloadingwastewatertreatmentWUHaoenWEICaijieWUWeizhong*CollegeofEnvironmentalScienceandEngineeringPekingUniversityBeijing100871Received26December2015receivedinrevisedform27July2016accepted29July2016AbstractTheMSLmulti-soil-layeringMSLsystemswithoneaerobicstageandoneanaerobicstageweresetupforthelowpollutantloadingwastewatertreatment．ThedenitrificationeffectinanaerobicstagewasenhancedbyexternalcarbonsourceadditionwhichwereselectedasthesawdustforMSL1systemandthePHBVpoly-3-hydroxybutyrate-co-3-hydroxyvaleratemixedGC-4carbonsourceforMSL2system．Operationconditionsincludingtemperatureandhydraulicloadingwerevariedforsystemperformanceevaluation．Whenthehydraulicloadingwas1．0m3·m－2·d－1thedenitrificationefficienciesofbothtwoMSLsystemsweredecreasedwhentheinflowtemperaturewasdecreasedfrom～19℃to＜15℃．TheaverageNH+4-NandTNremovalefficienciesofMSL2werereducedfrom91%to45%andfrom81%to62%respectively．ForMSL2systemtheaverageNH+4-NandTNremovalefficiencieswerereducedfrom88%to80%andfrom72%to55%respectively．ThereresultsdemonstrateanobviouslybetternitrogenremovalperformanceofMSL2systemwithGC-4comparingwithMSL1withsawdust．InadditiontheTNremovalefficienciesofbothtwoMSLsystemswereenhancedforabout20%underlowerhydraulicloadingindicatingthatamoresufficientcontactbetweentheslowerwaterflowandthecarbonsourcecanbenefitthedenitrificationefficiency．Basicallytherewerenoexcessivecarbonsourcereleasephenomenonoccurredsincetheaverage12CODremovalefficienciesofMSL2weremaintainedatabout40%．Thefunctiondivisionandabundancesofmicroorganismnitrificationbacteriaanddenitrificationbacteriawerestudiedbymolecularbiologicalapproach．TheresultsshowthatthebiomassandthedenitrificationgeneabundanceinMSL2systemwereobviouslymorethanthatinMSL1system．ThenitrificationbacteriaamoAgenewerefoundmainlydistributinginaerationstagewhiledenitrificationbacterianirSnirKmainlydistributedinthesoillayerofanaerobicstagewhichevidentlyexplainedthedenitrificationdifferencebetweentwoMSLsystemsinbiologicalaspect．Keywordsmulti-soil-layeringsystemsolidcarbonsourcelowpollutantloadingwastewaterenhancednitrogenremoval1Introduction．、2010．、．、、、2009．、2015．．、．．Multi-soil-layeringsystemMSLWakatsuki19902090、、、2009a．PLML“-”．、、、Luanmaneeetal．2001Luanmaneeetal．2002Satoetal．20052009b201020122011．MSL“-”．、、、、．MSL．．“+”MSLAC/NPHBV、GC-4MSL、MSL．2Materialsandmethods2．1“+”1．“A2/O”1MSLFig．1StructureandflowchartofexperimentMSLsystem393436．．．0．4m×0．3m×0．8m××0．4m×0．3m×1．0m××．ML2××=0．3m×0．2m×0．1m××=0．15m×0．2m×0．1m．PL40mm．2．22MSL1MSL2GC-4．2MSL1PLML、、、、、PLML、、、、、MSL2MSL1GC-4．2．3201482015615℃3．119．05℃＞15℃211．41℃319．03℃＞15℃．、MSL1．、2MSL．1Table1Summaryofthewaterqualityandoperationconditions/℃pHCOD/mg·L－1NH+4-N/mg·L－1NO－3-N/mg·L－1TN/mg·L－1/m3·m－2·d－1115．80～24．60/19．057．35～7．59/7．4518．64～37．29/27．886．98～9．91/8．0510．45～25．75/19．1817．94～34．60/28．231．02～5∶127．30～15．40/11．417．29～7．46/7．3717．50～42．50/27．4310．21～29．74/17．753．65～17．51/8．6222．36～35．60/28．091．02～5∶1315．60～23．50/19．037．39～7．73/7．5220．48～44．32/32．521．60～18．16/5．022．38～13．89/8．584．04～28．26/15．460．62～5∶1“/”．2．42NH+4-N、NO－3-N、TN、CODCr、pH、．0．45μm．NH+4-NNO－3-NTN-CODCr、pH2002．2．52MSLMSL“”PL、ML20-P、0-M2MSLMSL1、MSL2“”PL、ML4MSL1-P、MSL1-MMSL2-P、MSL2-M．DNA16SrRNAamoA、nirS、nirKPCR2015．3Experimentresultsanddiscussion3．12MSLMSL-、、、．“-”MSL．2．3．1．12“-”MSLNH+4-N、．NH+4-N1MSL．1MSL2MSL1NH+4-N88%、91%280%、81%．MSLNH+4-N．MSL．NH+4-N4934122、MSLNH+4-N、NO－3-N、TNTable2AverageremovalefficiencyofNH+4-NNO－3-NandTNNH+4-N/mg·L－1NO－3-N/mg·L－1TN/mg·L－1MSL1MSL2MSL1MSL2MSL1MSL218．05±0．820．76±0．2791%0．92±0．3888%19．18±5．847．39±1．9861%4．52±1．0975%28．23±5．5710．30±2．5062%7．59±2．0972%217．75±5．953．65±2．0081%3．75±1．8380%8．62±5．659．70±3．36－81%7．29±2．69－36%28．09±4．3615．50±5．1745%12．72±4．3855%35．02±4．120．32±0．3993%0．67±0．8989%8．58±3．635．62±2．9531%3．27±2．2261%15．46±5．506．62±3．2466%4．53±2．8981%MSLNH+4-N80%MSL．2a1315℃21．0m3·m－2·d－18mg·L－1MSL1、MSL20．76、0．92mg·L－10．6m3·m－2·d－15mg·L－1MSL1、MSL20．32、0．67mg·L－12MSL90%．MSL．22MSL13NH+4-N95%p1=0．076＞0．05p2=0．878＞0．05．3．1．22b2NO－3-N．NO－3-NNH+4-N1．22bMSL．2MSL12NO－3-Np1=p2=0．000＜0．05．1MSL1、MS