多菌种生物膜内各菌种生物量的动力学推定及验证孙国胜

31820118ActaScientiaeCircumstantiaeVol．31No．8Aug．2011No．2008ZX07211-003SupportedbytheNationalKeyScientificandTechnologicalProjectNo．2008ZX07211-0031971—[e-mail]*[e-mail]1971—[e-mail]*[e-mail]ofakineticmodelforthebiomassofdifferentbacterialstrainsinamulti-strainbiofilmJ．ActaScientiaeCircumstantiae3181627-1632121*1．5100062．5180212010-11-032010-12-102010-12-15Monod．、AOBNOBAOBNOB．AOBNOB、AOBNOBAOBNOB68．2%～74.2%25．0%～29．9%AOBNOB．0253-2468201108-1627-06X171ADevelopmentandvalidationofakineticmodelforthebiomassofdifferentbacterialstrainsinamulti-strainbiofilmSUNGuosheng12JIAXiaoshan1*1．SchoolofEnvironmentalScienceandEngineeringofSunYat-SenUniversityGuangzhou5100062．GuangdongYuegangWaterSupplyCO．LTD．Shenzhen518021Received3November2010receivedinrevisedform10December2010accepted15December2010AbstractAseriesofstaticexperimentsweredonetostudythebiomassdistributionalongthelengthofbio\contactoxidationtanksandtheeffectoflimitingsubstratesonammoniaoxidizingbacteriaAOBandnitriteoxidizingbacteriaNOBinDong-shenrawwaterbio-pretreatmenttanks．BasedontheMonodmodelakineticmodelofdifferentstrainsofbiomasswassetupandanalyzed．Inordertotestthebiomasskineticmodelcultivationexperimentswereconductedonthebio-filmtodetectandcountAOBsandNOBsandoutflowsimulationexperimentswerealsoundertaken．ThestudyshowedthatthemethodsofthekineticsmodelbasedontheAOBandNOBbiomassinthebiofilmweresimpleandfeasible．TheAOBandNOBbiomassinthebiofilmwaslowatbothendsandhighinthemiddleofthebiotank．TheactivebiomassofAOBandNOBaccountedfor68．2%～74．2%and25.0%～29．9%ofthetotalbiomassrespectively．Thebiomasskineticmodelwasusedtoinfertheoutflowofthebiofilmreactorwithexcellentsimulationefficiency．Keywordsbiofilmreactoractivebiomasskineticsmodelnitrificationnitrosation1Introduction2004．、2007．．Findlay90%～98%DOI:10.13671/j.hjkxxb.2011.08.00431Yuetal．2002．Yuetal．2002UrferBRPUrferetal．2001．2003．Caoetal．2002．．．、．．、AOBNH+4-NNOBNO－2-N．．．AOB、NOB．20032004MonodMPBSRB、、．、AOB、NOB．．、400t·d－1．．2Settingupthemodelforinferringbiomass、2006．2．1、Chandranetal．2000－dSNHdt=νNHmaxSNHKNHNH+S[]NHSOKNHO+S[]OXAOB1－dSNOdt=νNOmaxSNOKNONO+S[]NOSOKNOO+S[]OXNOB2dSNHdt、dSNOdtNH+4-NNO－2-Nmg·L－1·h－1KNHNH、KNHOAOBNH+4-N2．36mg·L－10．6mg·L－1KNONO、KNOONOBNO－2-N5．5mg·L－12．2mg·L－12006SNH、SNONH+4-NNO－2-Nmg·L－1SODOmg·L－1νNHmax、νNOmaxNH+4-NNO－2-Nmg·mg－1·h－1XAOB、XNOBAOBNOBmg·L－1．2．22．2．1XAOB200320041KNHNHlnSNHSNH0+SNH－SNH0=－νNHmaxSOKNHO+SOXAOBt3SNH0NH+4-Nmg·L－1．t1、t2XAOBKNHNHlnS1NHSNH0+S1NH－SNH0=－νNHmaxSOKNHO+SOXAOBt14KNHNHlnS2NHSNH0+S2NH－SNH0=－νNHmaxSOKNHO+SOXAOBt25XAOB82618XAOB=KNHNHlnS1NHS2NH+S1NH－S2NH－νNHmaxSOKNHO+SOt1－t26XAOBX1AOBX2AOB=KNHNHlnS1NHSNH0+S1NH－SNH0KNHNHlnS2NHSNH0+S2NH－SNH072．2．2XNOBNH+4-NNH+4-NNO－2-NdSNOd()t=dSNOd()t－dSNOd()t8NH+4-NNH+4-NNO－2-NNO－2-NNO－2-NNO－2-NdSNOd()t=09dSNOd()t=dSNOd()t10νNHmaxSNHKNHNH+S[]NHSOKNHO+S[]OXAOB=νNOmaxSNOmaxKNONO+SNO[]maxSOKNOO+S[]OXNOB11XNOB=νNHmaxSNHKNHNH+S[]NHSOKNHO+S[]OνNOmaxSNOmaxKNONO+SNO[]maxSOKNOO+S[]OXAOB12NO－2-NNH+4-N12XNOBXAOB．6、7、12XAOB、XNOB．3Materialsandmethods3．16270m×25m×5m××3．8mHRT55．4min．251．5m、．NH+4-N≤3mg·L－1CODMn≤5mg·L－1BOD5≤4mg·L－1．3．23．2．16．0mg·L－1NH+4-N30℃、10～20min．3．2．2125～30℃10～20d2005AOBNH42SO42．0gMnSO40．01gMgSO40．03gNaH2PO40．25gK2HPO40．7gCaCl20．2g1000mL．NOBNaNO21gNaH2PO40．25gMgSO40．03gK2HPO40．75gNaCO31．0gMnSO40．01g1000mL．3．2．3221CSTRMonodNH+4-NNO－2-NDO、NH+4-N、NO－2-NNO－3-N．2009．4Experimentalresultsandverification4．14．1．1XAOB、XNOB1NO－2-NNH+4-N6、7、12AOB、NOB1．1Table1Activebiomassoftestcarrier/mAOB/mg·L－1NOB/mg·L－1139．315．17812．724．22708．421．09261311XAOBXNOBT=30℃Fig．1TestandcalculationofXAOBandXNOBT=30℃4．1．2XAOB、XNOBXAOBXNOB2．22．2AOB、NOBTable2CalculatedresultsoftheconcentrationsofAOBandNOB/m/mg·L－1/mg·L－1AOB/mg·L－1/mg·L－1NOB/mg·L－1133．103．069．30．22515．1394．82．8011．7655．512．5112．5785．72．3812．70．57624．2915．852．2512．61176．062．0011．90．62225．01566．251．6611．00．60024．62086．51．309．80．52423．02706．750．998．30．42821．02Fig．2BiologicalconcentrationalongthelengthofthetankNH+4-N0～60mAOBDODONH+4-NNH+4-NNOB．AOBXAOB=5．5S1/2ONOBXNOB=32S1/2NO036184．21AOB、NOB1×10－9mg·－11#、2#AOB、NOB3．1#、2#、、2006．NOBAOB．AOB68．2%～74．2%NOB25．0%～29．9%．．31#2#Fig．3BiologicalconcentrationalongthelengthoftheNO．1andNO．2tanks4．32Monod、30℃3．8m1．2～1．4∶1．、、4．4DO、NH+4-N、NO－2-NNO－3-NT=30℃Fig．4ComparisonofDONH+4-NNO－2-NandNO－3-NsimulationresultsT=30℃R20．8、R20．6．5Conclusions1136131AOBNOB、．2AOBNOBAOBNOB68．2%～74.2%25.0%～29．9%．3、、．AOBXAOB=5．5S1/2ONOBXNOB=32S1/2NO．4AOBNOB．1962—[e-mail]yandEngineeringChina55101690-1695inChineseCaoHBJiangBLiXAetal．2002．NovelmethodextracellularpolymericsubstancesfromintactbiofilmsJ．JournalofChemicalIndustryandEngineeringChina53121300-1302ChandranKSmetsBF．2000．Single-stepnitrificationmodelserroneouslydescribebatchammoniaoxidationprofileswhennitriteoxidationbecomesratelimitingJ．Biote