多维度耦合丝状菌监测方法在污泥膨胀中的应用范念斯

1，2，1，ValterTandoi3，1(1．，100085;2．，100022;3．，00015)。(SVI)，———。，、，SVI，，(Microthrixparvicella)、。PCＲ，，、、，。;;;;;[e-mail]-dimensionalIntegratedFilamentousMonitoringMethodinSludgeBulkingDynamicProcessFANNian-si1WANGＲun-fang2QIＲong1ValterTandoi3YANGMin11．ＲesearchCenterforEco-EnvironmentalSciencesUniversityofChineseAcademyofSciencesBeijing100085China2．WaterQualityTestingCenterBeijingDrainageWaterEnvironmentDevelopmentCo．Ltd．Beijing100022China3．WaterＲesearchInstituteCNＲＲoma00015ItalyAbstractActivatedsludgebulkingphenomenacausedbyabundantfilamentsisoneofthereasonsfortheseparationproblemofsedimentationtankandunstablenutrientsremovalperformancesinfull-scalemunicipalwastewatertreatmentplants．ThesludgevolumeindexSVImethodiskeytodeterminethebulkingstatushowevercannotprovideessentialfilamentousdynamicinformation．Multi-dimensionalin-tegratedmethodwasadoptedtomonitorthesludgepropertyfilamentouscommunitystructureanddomi-nantspeciesdynamicsduringthecompletebulkingprocessandpredicatedtheresultsofcontrolstrategycarryingoutinthefull-scaleplant．AtdifferentbulkingstagesdividedbySVIvaluestheintegratedmethodnotonlyefficientlycollectthevaluableinformationoffilamentousbacteriadynamicsbutalsopro-videvisibleandaccurategrowthpositionofabundantfilamentousbacteriaparticularlydominatedMicro-·6·331520178CHINAWATEＲ＆WASTEWATEＲVol．33No．15Aug．2017thrixparvicellawhichweremicrobialevidencestoevaluatetheeffectsofbulkingcontrolstrategy．ThequantitativeexperimentsshowedthattheintegratedmethodresultswereequaltothequantitatePCＲqPCＲmethod．Consideringtheadvantagesofintegratedmethodsuchashighefficiencypracticalop-erabilityandlowcostitisbeneficialtowidelyapplythemethodinfull-scalemunicipalwastewatertreat-mentplants．KeywordsactivatedsludgesludgebulkingfilamentMicrothrixparvicellaintegratedmethodbulkingcontrol。。SVISVI≥150mL/g1。SVIPCＲqPCＲ、16SrＲNA、2～5、。。、。SVI6。SVI。FI7、SVIPCＲ。、。11.1TaqDNAdNTPMix10×LoadingBuff-erDNA。MLS－3750Mastersizer2000System9700PCＲABI7300PCＲOLYMPUSBX51AxioCamMＲmCCD。1.2A2/O。DNA。DNA100μLDESDNANanoDropDNA。DNA－20℃。1.3。50μL。10×100×。。、、、。1A2g20mL、95%B0．8g80mL21g2g300mL310mL2．5%95%100mL。1A·7·．watergasheat．com，:3315B2∶1A0．1g、5mL95%、5mL100mLB3．3mL10%95%、6．7mL95%100mL233．3mL1%66．7mL。1.4SVIFICOD、NH+4－N、NO－3－N、NO－2－N、PO3－4－P、SVI、MLSS。Jenkins。。1。1Tab．1ClassificationcriteriaforFIFI/0None1Few2Some31～5Common46～20Verycommon520Abundant6Excessive1.5PCＲPCＲqPCＲ。PCＲNanoDropDNA。PCＲDNA－20℃。8=DNA/DNA×6．02×1023×10－91DNA=660×+2SYBＲ－GreenⅠPCＲMicrothrixparvicella。S－S－M．par－0828－S－21S－S－M．par－1018－A－17395℃10min95℃15s62℃30s60℃30s40。16SrＲNAS－D－Bact－0509－S－17S－D－Bact－0784－A－22994℃5min94℃5min60℃30s72℃30s40。PCＲＲ2＞0．99。22.1201310—201410A2/O12。SVIⅠ、Ⅱ、Ⅲ、ⅣⅤ51。201310—11SVI＜150mL/g201312—20141SVI200mL/g20142—4SVI220mL/g20145—7SVI720148—10SVI120mL/g。1201310—201410SVIFig．1SVIvariationcurvefromOctober2013toOctober2014SVI·8·3315．watergasheat．comCODNH+4－NNO－3－NNO－2－N2。2201310—201410Fig．2NitrogencompoundsconcentrationsvariationineffluentofWWTPfromOctober2013toOctober201432Ⅰ≥200μm1～2。Type00411～1．2μm、、Type00921．2μm、SVI120mL/g。3Fig．3BrightfieldandGram＆NeisserstainingmicroscopicimagesoffilamentousbacteriadynamicsduringentirebulkingprocessSVIType0092Type0041M．parvicella·9·．watergasheat．com，:3315。SVI。2201310—201410FITab．2FilamentindexFIstatusofactivatedsludgefromOctober2013toOctober2014FI/M．parvicellaType0041Type0092Type021NN．limicolaⅠ1230122310Ⅱ3230132301422114220141100Ⅲ51100511005120052301Ⅳ3240112401Ⅴ124012340122410ⅡSVI。SVI150mL/g1～22～3150μm100～150μm。Nos-tocoidalimicola、ThiothrixeikelboomiiType021N。ⅠType0092Type0041Type0041M．parvicella。SVI。Ⅱ20/＜100μmType0092Type0041M．parvicella、SVI150mL/g。。SVI、SVI。Ⅲ＜50μm。M．parvicellaType0092Type00411。SVI。Ⅳ2。M．parvicellaType0092Type0041。SVI150mL/g。ⅤⅠ。SVI。SVI。、M．parvicellaSVI。SVISVI。M．parvicella4。·01·3315．watergasheat．comSVI180mL/gⅡM．parvicella。M．parvicella。41000×Fig．4Gram-stainingimages1000×ofM．parvicellainstageofinitialsludgebulking。7dSVI175mL/gM．parvicella0．6～0．8μm。3～4SVI150μmM．parvicellaM．parvicella。。、、。2.2PCＲPCＲM．parvicella5。5M．parvicella、FISVIFig．5VariationofM．parvicellarelativeabundanceFIandSVIvaluesduringbulkingperiod5M．parvicellaSVI。ⅠSVI120mL/gqPCＲM．parvicella3%1M．parvicella。ⅡSVI150mL/gM．parvicella10%3～4M．parvicella。ⅢSVI192mL/gM．parvicella30．97%5。M．parvicella。。。。·11·．watergasheat．com，:3315。3①FI、SVI、、。②SVI250mL/g。SVIⅠⅡSVI、。③PCＲ。、、。1LiaoJLouIFrancisL．Ｒelationshipofspecies-specificfilamentlevelstofilamentousbulkinginactivatedsludgeJ．ApplEnvironMicrobiol20047042420－2428．2VervaerenHDeWildeKMatthysJetal．QuantificationofanEikelboomtype021Nbulkingeventwithfluores-cenceinsituhybridizationandreal-timePCＲJ．ApplMicrobiolBiotechnol2005685695－704．3KaetzkeAJentzschDEschrichK．QuantificationofMi-crothrixparvicellainactivatedsludgebacterialcommunit-iesbyreal-timePCＲJ．LettersApplMicrobiol2005403207－211．4KumariSSMarrenganeZBuxF．Applicationofquanti-tativeＲT－PCＲtodeterminethedistributionofMicro-thrixparvicellainfull-scaleactivatedsludgetreatmentsystemsJ．ApplMicrobiolBiotechnol20098361135－114