二级出水内毒素活性及紫外线消毒对其的影响

112111．1000842．518055———。。103EU/mL。52%～92%。＜150mJ/cm2。X703A1000－4602201107－0033－04EndotoxinActivityofSecondaryEffluentandInfluenceofUVDisinfectiononItHUANGHuang1HUHong-ying12YANGYang1WUQian-yuan11．StateKeyJointLaboratoryofEnvironmentSimulationandPollutionControlSchoolofEnvironmentTsinghuaUniversityBeijing100084China2．GraduateSchoolatShenzhenTsinghuaUniversityShenzhen518055ChinaAbstractEndotoxinsarecomponentsincellwallsofGram-negativebacteriaandareconsideredasriskfactorsforwastewaterreusebecauseoftheirinflammatorycapacity．ThechromogenicLimulusa-mebocytelysateLALendpointassaykitswereusedtoanalyzetheendotoxinactivityofsecondaryefflu-entfromWWTPandtheinfluenceofUVdisinfectiononendotoxinactivityinsecondaryeffluentandE．coliwasinvestigated．Theresultsshowthatendotoxinactivityofabout103EU/mLcanbedetectedinsec-ondaryeffluent．Freeendotoxinactivityis52%to92%oftotalendotoxinactivityinsecondaryeffluentindicatingthatfreeendotoxinsaremainformsofendotoxinsinsecondaryeffluent．FurthermoreendotoxinactivityinsecondaryeffluentandE．colidoesnotchangesignificantlyafterUVdisinfectionsuggestingthatUVdisinfectionofbelow150mJ/cm2cannotremoveendotoxinseffectively．KeywordsendotoxinUVdisinfectionsecondaryeffluent508258018632008AA062502、。。LPS12。·33·27720114CHINAWATER＆WASTEWATERVol．27No．7Apr．2011、、、、、3。。———。11.1250℃1h、96。PBSMilli－Q。1.2AB。A2O。4℃12h。1.3CGMCC1．3373。37℃、150r/min16h10000r/min10minPBSpH7．4。。1.4A254。15mL60mm140W4。1.5。O111B4。0．1～1EU/mL。1∶100～1∶1000002～3。37℃8min6min。A405。tp＜0．05。。。12000g10min。。1.6。1mL≤40℃37℃24h。3。t。22.1AB351。1Tab．1EndotoxinactivityinsecondaryeffluentEU·mL－1137713226002510A113710396001772B152214168152510600～2510EU/mL1377EU/mL。A、B1137、1522EU/mL。A2O。102～104EU/mL56。103EU/mL。、783。·43·277．watergasheat．com1。52%～92%。A78%B60%。1Fig．1Proportionsoffreeandcell-boundendotoxininsecondaryeffluent2.2A0、20、50、100150mJ/cm22“×”。2Fig．2EffectofUVdisinfectiononendotoxinactivityandtotalbacteriacountinsecondaryeffluent3800CFU/mL600EU/mL。p＜0．0550mJ/cm25CFU/mL。p＞0．05。150mJ/cm2。Anderson9LPS100～600mJ/cm2。。Anderson。Gehr40、100mJ/cm23。10～150mJ/cm2。3。108CFU/mL103CFU/mLp＞0．05。10。3Fig．3EffectofUVdisinfectiononendotoxinactivityandcellcountsinE．coli。。3①103EU/mL52%～92%。②150mJ/cm2。1WilliamsKL．EndotoxinsPyrogensLALTestingand·53·．watergasheat．com277DepyrogenationM．NewYorkInformaHealthcareInc2007．2GehrRUribePUBaptistaIFDSetal．Concentra-tionsofendotoxinsinwatersaroundtheIslandofMont-realandtreatmentoptionsJ．WaterQualResJCan2008434291－303．3MorrisonDCDannerRLDinarelloCAetal．Bacteri-alendotoxinsandpathogenesisofgram-negativeinfec-tionscurrentstatusandfuturedirectionJ．JEndotox-inRes19941271－83．4．J．20082992576－2579．5GuizaniMDhahbiMFunamizuN．Assessmentofendo-toxinactivityinwastewatertreatmentplantsJ．JEnvi-ronMonit20091171421－1427．6BurgerJSGrabowWOKKfirR．Detectionofendo-toxininreclaimedandconventionallytreateddrinking-waterJ．WaterRes1989236733－738．7ParikhSJChoroverJ．InfraredspectroscopystudiesofcationeffectsonlipopolysaccharidesinaqueoussolutionJ．ColloidSurfBBiointerface2007552241－250．8Mattsby-baltzerILindgrenKLindholmBetal．Endo-toxinsheddingbyenterobacteriafreeandcell-boundendotoxindifferinLimulusactivityJ．InfectImmun1991592689－695．9AndersonWBHuckPMDixonDGetal．Endotoxininactivationinwaterbyusingmedium-pressureUVlampsJ．ApplEnvironMicrobiol20036953002－3004．10ChoMKimJKimJYetal．MechanismsofEscherich-iacoliinactivationbyseveraldisinfectantsJ．WaterRes201044113410－3418．1986－。[e-mail]櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵櫵2010－11－2932B/CHRT＞13．5hB/C。③“—”6．5m/hCODSS65%。④/COD、pH、NH3－N、TP95．3mg/L、8．27、6．27mg/L、0．39mg/L。1．SBRJ．2004305500－504．2．J．2006255135－139．3．A2/O2J．2010261290－92．4．J．2010262391－93．5．UBFUASBJ．2007371144－147．6．/UBF//BAFJ．2008242263－67．7．UBFJ．200625244－47．1981－。[e-mail]2010－11－10·63·277．watergasheat．com