反硝化聚磷机理试验王春英

王春英1　隋　军2　赵庆良3(1.,510300;2.,510060;3.,150090)　　,DPB、CODCr。,DPBNO-3,。　　　　　StudyonmechanismofdenitrifyingdephosphatationWangChunying1　SuiJun2　ZhaoQingliang3(1.GuangzhouGuangyiGroupCo.Ltd,Guangzhou510300;2.GuangzhouMunicipalEngineeringDesignandResearchInstitute,Guangzhou510060;3.DepartmentofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090)Abstract　ThelaboratorystudyonanoxicdenitrifyingdephosphatationrevealedtheregularityofCODCrandhowtheDPBbacteriatakeuporreleasephsphateunderbothanaerobicenvironmentandanoxicenviron-mentbyturns.ItshowedthattheDPBbacteriaunderanoxicenvironmentemploythenitrateastheelectronacceptortouptakephosphate,andbettereffciencyofphosphorusuptakingandfasterratesofdenitrifyingwereobserved.Keywords　biologicalwastewatertreatment;nitrogenandphosphorusremoval;anaerobic;anoxic;denitrifyingdephosphatation1　　(A2/O、A2/O、UCT),PAO,“(poly-P)”-PAO(poly-phosphateac-cumulatingorganisms)/[1,2]。PAO/,[3,4],()PHA(-β--),;PAO,PHA(O2),,,。PAO/,poly-P。[5],PAO,———DPB(denitrifyingphosphorusremovingbacteria)。DPBPAO,PHAO2NO-3,NO-3-NN2,NO-3-O,DPB,poly-P,。,。,。2　2.1　、、,6,1。36Vol.3,No.620026TechniquesandEquipmentforEnvironmentalPollutionControlJun.,20021　　pHNO-3-N(mg/L)NH+4-N(mg/L)TN(mg/L)PO3-4-P(mg/L)TP(mg/L)CODCr(mg/L)BOD5(mg/L)6.8—7.40.78—0.8627.49—36.5731.81—42.812.66—3.883.54—5.06120—37874—228　　:《》[6]。1　2.2　,“”,1,MLSS=2500—5000mg/L。2.3　(),1∶2,,,CODCr。,KNO3,,。2。3　23,,。2(a)180minKNO3;2(b)180minKNO3,(a)2;2(c)60minKNO3,2/3。2(a),,10min,,CODCr,265mg/L128mg/L,52%。,CODCr。30min,2　CODCr、PO3-4-PNO-3-N,CODCr。,PO3-4,CODCr,10min,3.90663mg/L6.01mg/L,8.33gPO3-4-P/kgMLSS·h,,50min,6.01mg/L7.74mg/L,1.37gPO3-4-P/kgMLSS·h,。DPB,PHA,PO3-4。180min,PO3-49.26mg/L,CODCr127mg/L,KNO3,DPBNO-3PHA,15min,PO3-42.97mg/L,16.55gPO3-4-P/kgMLSS·h;,PO3-4-P:2.97mg/L0.5mg/L1h,1.63gPO3-4-P/kgMLSS·h。,,CODCr,NO-3,NO-3-NN2,,90min,NO-326.2mg/L7.96mg/L,8gNO-3-N/kgMLSS·h。90min,,PO3-4-P0.33mg/L,PO3-4,NO-3,,,NO-37.96mg/L4.95mg/L,3.96gNO-3-N/kgMLSS·h。,PO3-4,NO-3。2(b),DPBCODCr,2(a):CODCr,PO3-4,;,PO3-4,,PO3-4,PO3-4。2(b),,10minCODCr45%,3.26gPO3-4-P/kgMLSS·h,10—40min2.4gPO3-4-P/kgMLSS·h。,5min34.76gPO3-4-P/kgMLSS·h,PO3-4-P=2.66mg/L,0.5mg/L,1.48gPO3-4-P/kgMLSS·h;NO-390min,12.46gNO-3-N/kgMLSS·h,PO3-4-P=0.22mg/L,,1.94gNO-3-N/kgMLSS·h。2(b)(a),KNO3:(a)NO-3-N=26.20mg/L,(b)NO-3-N=50.15mg/L。(b)KNO3,,90min,NO-3-N,22.30mg/L。2(c)60min,,KNO3,PO3-4(a)(b),5min,6.88mg/L0.78mg/L,29.28gPO3-4-P/kgMLSS·h,16.32gNO-3-N/kgMLSS·h,;,60min,60min。NO-3,CODCr(120mg/L)。A/A/O5.99gNO-3-N/kgMLSS·h[7](34%)。CODCr:,,。(15—30min),CODCr。,PO3-4-PNO-3-N,,PO3-4-P5—15min,PO3-4-P=2—3mg/L;NO-3-N30—60min,NO-3。PO3-4NO-3,PO3-4PO3-4。4　　(1)DPB:;,NO-3-NN2,。(2)DPBCODCr:5—10minCODCr30%—50%,676:10—15min3.25—8.33gPO3-4-P/kgMLSS·h,15—90min0.53—2.4gPO3-4-P/kgMLSS·h;60min。(3)DPB,PO3-4。5—15min16.55—34.76gPO3-4-P/kgMLSS·h,PO3-4-P5—15min2—3mg/L,1.04—1.63gPO3-4-P/kgMLSS·h,PO3-4-P0.5mg/L,1h。(4)NP,NO-3,,PO3-4;,NO-3,NO-3。(5),A/A/O34%。[1]BortoneG,etal.Biologicalanoxicphosphorusremoval-thedephanoxprocess.Wat.Sci.Tech.,1996,34(1—2):119—128[2]MinoT,VanLoosdrechtMCM,HeijnenJJ.Mcrobiologyandbiochemistryoftheenhancedbiologicalphosphatere-movolprocess.WaterResearch,1998,32:3193—3207[3],..,1998,14(5):1—3[4]..,1999,17(5):17—18[5]KubaT.MurnleitnerE.,etal.Ametabolicmodelforthebiologicalphosphorusremovalbydenitrifyingorganisms.Biotech.Bioeng.,1996,52:685—695[6].().:,1998[7],.//.,1999,18(1):19—21(责任编辑:刘　颖)(上接第71页)[4]Nagadomi,H.,Takahasi,T.Simultaneousremovalofchemicaloxygendemandnitrateinaerobictreatmentofsewagewastewaterusinganimmobilizedphotosyntheticbacteriumofporousceramicplates.WorldJournalofMi-crobiology&Biotechnology,2000,16(1):57—62[5]..,1999,20(1):39—42[6]..,1995,14(4):10—13[7]..,2000,19(5):225—227[8]..,2001,21(2):189—193[9]..,2001,20(2):120—122[10]..,1999,6(4):55—58[11]..,1999,12(4):233—234,248[12]J.delaNouee,D.Proulx.Tertiarytreatmentofurbanwastewatersbychitosan-immobilizedphormidiumsp.Presentedat4.InternationalMeetingoftheSocietyforApplliedAlgology,Villeneuved'Ascq(France),15—17Sep1987.Publ.by:ELSEVIERAPPLIEDSCIENCE,BARKING,ESSEX(UK),1988,159—168[13]..,1995,15(1):10—13[14]CanizaresRO,etal.Freeandimmobilizedculturesofspirulinamaximaforswinewastetreatment.Biotechnol.Lett.,1993,15(3):321—326[15]TraviesoL,etal.Experimentsonimmobilizationofmi-croalgaefornutrientremovalinwastewatertreatment.BiosourceTechnol.,1996,55(3):181—186[16]CodobaLLandSanchez-HernandezE.Finaltreatmentforcattlemanureusingimmobilizedmicroalgae.Istudyofthesupportmedia.Resour.Conserv.Recycl.,1995,13(3—4):167—182(责任编辑:刘　颖)683