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2009,21(10):117～119ActaAgriculturaeJiangxiC18吴守中,蔡天明＊,陈立伟 :2009-08-17:(1985-),,,,:。＊:。(,210095) :从城市生活污水处理厂好氧池活性污泥中筛选出的一株反硝化聚磷菌C18,经16SrDNA初步鉴定为假单胞菌(Pseudomonasgrimontii)。C18在pH6.5～7.5之间能正常生长,pH为7.5时,脱氮除磷效果最好。C18生长对温度没有特殊要求,当温度为30℃时,磷和氨氮去除率分别达到85.9%和83.6%。厌氧/缺氧最佳连续培养时间为厌氧2h、缺氧4h。:反硝化聚磷菌;假单胞菌;脱氮除磷:X703 :A :1001-8581(2009)10-0117-03NitrogenandPhosphorusRemovalCharacteristicsofDenitrifyingPolyphosphate-accumulatingOrganismC18WUShou-zhong,CAITian-ming＊,CHENLi-wei(CollegeofResourcesandEnvironmentalScience,NanjingAgriculturalUniversity,Nanjing210095,China)Abstract:Adenitrifyingpolyphosphate-accumulatingorganismC18wasisolatedfromaerobicactivatedsludgeinamunicipalsewagetreatmentfactoryandidentifiedasPseudomonasgrimontii.ThisstraincouldnormallygrowundertheconditionofpH-value6.5～7.5,andithadthebesteffectinnitrogenandphosphorusremovalunderpH-value7.5.C18grewwithnospecialrequirementsfortemperature,whenthetemperaturewas30℃,phosphorusandammonianitrogenremovalratereached85.9%and83.6%respec-tively.Anaerobicculturefor2hoursandanoxicculturefor4hourswerethebestforitscontinuousculture.Keywords:Denitrifyingpolyphosphate-accumulatingorganism;Pseudomonasgrimontii;Nitrogenandphosphorousremoval ,、[1,2]。,A2/O、SBR、、UCT、Phostrip、VIP[3～5]。:;、,,[6,7]。,(Denitrifyingpolyphosphate-accumulatingorganisms,DNPAOs)。,NO3-O2PHB,PHBPO43-,,()[8,9]。C18,,。1 1.1 1.1.1 LB培养基[10] 5g、10g、NaCl5g、1000mL、pH7.0～7.2。1.1.2 菌株 C18。1.1.3 试验设备 SBR(SequencingBatchReactor),,2L,5。(2～3L/min)0.2mg/L,0.2～0.5mg/L。1.1.4 试验用水 (1),COD、、NH4ClK2HPO4。1 (g/L)300mgCOD/LZnSO40.14NH4Cl16mgN/LCoCl20.15K2HPO410mgP/LCuSO40.03NaHCO350mg/LMnCl20.12MgSO445mg/LFeCl30.14NaCl15mg/LH3BO30.151.2 1.2.1 菌株的鉴定 16SrDNA:《()》[11]。,16SrD-NA[11～12]。1.2.2 厌氧/缺氧连续培养时的脱氮除磷效果 DOI:10.19386/j.cnki.jxnyxb.2009.10.036SBR/,2h,4h,、pH、,OD600,COD、,。:C188LB(3mL),200mLLB,30℃8h,。1.2.2.1 最适pH试验 2%5SBR,NaOHpH5.5、6.5、7.0、7.58.5,/,,OD600,,。1.2.2.2 最佳温度试验 2%5SBR,NaOHpH7.5,10、20、30、3545℃,/,,OD600,,。1.2.2.3 厌氧/缺氧最佳反应时间试验 1～5/0.5h/1h、1h/2h、2h/4h、3h/6h、4h/8h,,,,。1.2.3 分析方法[13～14] PO43--P,NO3--N,NH4+-N,DO。2 2.1 C1816srDNA 16SrRNAC18PCR,1.5kbPCR,。RDPC1816SrDNAGenBank16SrRNA,(Pseudomonasgrimontii)16SrRNA99%,C18(Pseudo-monasgrimontii)。C181、2。1 C182 C18(×1000)2.2 pH 3,C18pH6.5～7.5。pH6.58.5,,。pH7.5,C18,OD600,10mg/L1.3mg/L,16mg/L2.5mg/L,87%84.4%。3 pHC182.3 4,C1830～35℃,,。30℃,10mg/L1.41mg/L,16mg/L2.62mg/L,85.9%83.6%。20℃30℃,,,10℃,。4 C182.4 / /,118 21,,。5,/0.5h/1h2h/4h,,35.7%88.6%,30.5%80.7%。2h/4h4h/8h,,。2h4h,1h。6,,2h10mg/L12.9mg/L,1.45mg/L·h,,1.14mg/L,88.6%。/2h/4h、。5 C186 2h4h3 (1)C18(Pseudo-monasgrimontii)16SrRNA99%,(Pseudomonasgrimontii)。(2)C18pH6.5～7.5,pH7.5,C18,OD600,87%84.4%。(3)C1830～35℃,,30℃,10mg/L1.41mg/L,16mg/L2.62mg/L,85.9%83.6%。C18,。(4)/,2h4h,88.6%80.7%。/2h/4hC18、。:[1]SeviourRJ,MinoT,Onuki.Themicrobiologyofbiologicalphosphorusremovalinactivatedsludgesystems[J].FEMSMicro-biologyReviews,2003,27:99～127.[2]ToerienDF,GerberA,LotterLH,etal.Enhancedbiologicalphosphorusremovalinactivatedsludge[J].Adv.Microb.Ecol.,1990,11:173～230.[3],,.[J].,1996,12(5):32～33.[4],.[M].:,1998.209～213.[5],.[J].,2003,24(3):180～183.[6]DEA.Sunglee,CHEOkJeon,JONGMoonPark.Biologicalnitro-genremovalwithenhancedphosphateuptakeinasequencingbatchreactorusingsinglesludgesystem[J].WatRes,2001,35(16):3968～3976.[7]JohwanAhn,TomotakaDaidou,SatoshiTsuneda,etal.Character-izationofdenitrifyingphosphate-accumulatingorganismsculti-vatedunderdifferentelectronacceptorconditionsusingpolymer-asechainreaction-denaturinggradientgelelectrophoresisassay[J].WaterRes,2002,36:403～412.[8]KubaT,vanLoosdrechtMCM,HeijnenJJ.Phosphorusandnitro-genremovalwithminimalCODrequirement,byintegrationofde-nitrifydephos-phatationanddenitrificationinatwo-sludgesystem[J].WaterRes,1996,30(7):1702～1710.[9]MMerzouki,NBernet,JPDelgenes,etal.Bioloicaldenitrif-yingphosphorusremovalinSBR:effectofaddednitrateconcen-trationandsludgeretentiontime[J].Wat.Sci.Tech,2001,43(3):191～194.[10]NeidhardtFC,BlochPL,SmithDF.Culturemediumforen-terobacteria[J].J.Bacterial.,1974,119:736～747.[11]SambrookJ,RussellDW.MolecularCloning:ALaboratoryMannual.3rdEd[J].ColdSpringHarborLaboratoryPress,2001.[12],.DLL[J].,2003,40(2):293～300.[13],,,.[J].,2003,25(6):323～325.[14],,,.-O/A[J].,2009,31(3):40～43.119 10 :C18