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394()Vol.39No.420088J.Cent.SouthUniv.(ScienceandTechnology)Aug.2008SBR(510225)DPBSBR\94752(SV)0.6319.32X172A1672−7207(2008)04−0705−07ChangeofmicroorganismamountandspeciesinSBRreactorenrichingdenitrifyingphosphatebacteriaZHOUKang-qun,LIUHui,SUNYan-fu,WANGBao-e,ZHOUYi-pin(DepartmentofEnvironmentScienceandEngineering,ZhongkaiAgrotechnicalCollege,Guangzhou510225,China)Abstract:BasedontheDPB(denitrifyingphosphorusremovalbacteria)principal,theSBR(sequencingbatchreactor)reactorwasusedtoenrichDPB.Thechangeofmicroorganismamountandspeciesduringdifferentphaseswerestudied.TheresultshowsthattheamountsofPAO(phosphateaccumulationorganism)andDPBincrease94and75times,respectively.Afterthesecondphase,theroutinePAOandpartialactinomycesareeliminated.ThechangeofSV(sludgesettingratio)providedtheindicationofthebacteriachange.Theamountofnitrateandnitritebacteriaduringtheaerobicphaseislargerthanthatofanaerobicphase.Theamountofnitritebacteriadecreases0.63timesbutthenitratebacteriaincreases19.3times.TheamountofdenitrifyingbacteriaandPAOismorethanthatofDPB.Theamountofdenitrifyingbacteriarisesfirstbutthendrops.Theroutinedenitrifyingbacteriaareeliminatedduringthesecondphase.Thereactorhassomezymogenandacetogen,butnomethanogenbacteria.AfterenrichmentthespeciesofPAOarereducedandconcentrated.DPBincludesPseudomonasspp/Corynebacteriumspp/EnterobacteriaceaesppandStaphylococcusspp.Keywords:nitrate;denitrifyingphosphatebacteria;microorganismamount;microorganismspeciesDPB(Denitrifyingphosphorusremovalbacteria)PHB(β-)22007−12−092008−02−27(04009663)(1963−)020-89003191E-mail:gzzkq@126.com()39706[1−2][3−4][5]DPBSBR311.1SBRSBR35L112345COD671SBRFig.1DynamicSBRreactor1.2SBRSBR21.31.3.112SBRFig.2TechnologyflowofSBR1Table1Compositionofwastewaterusedduringexperimentalperiod/(mg·L−1)COD150~378BOD580~235+4NH−N10.2~21.5−34PO−P3.8~6.8TN12.4~51.2TP4.8~9.3pH6.8~7.41.3.2SBRA2/O1.3.31(3.5~4.0g/L)1SBR2SBR3−3NO(DPB)1681/202////103(COD1)(ρ(−3NO−N)׃ρ(−34PO−P)=2׃1[6])3//4524SBR707SBR1.3.4a.[7]b.1[7]c.[8]1.41.4.11.3.31.3.43[7]1.4.21.4.13~41.4.3[9]1.4.2()1.4.5SUNTEXTS−2−3NO−NCOD5B−1pH98127pH22.11223217.2105/mL6.8107/mL93−34PO−PCOD63.4%51.8%79.6%98.0%31.1103/mL8.2104/mL74−34PO−P−3NO−NCOD9.8%46.56%58.6%95.2%96.1%81.5%β-(PHB)H+21(PAO)CODTable2ChangeofPAO/CODandphosphateremovalefficiencyduringthefirsttypicalcycle2l6121620PAO/(·mL−1)7.21058.71042.71055.71056.8107COD/%63.462.168.269.379.6/%51.880.085.193.298.032(DPB)CODTable3ChangeofDPB/COD/nitrate/phosphateremovalefficiencyduringthesecondtypicalcycle31022405897103DPB/(·mL−1)1.11032.11031.21042.51042.11044.31048.2104COD/%58.662.169.969.379.686.081.5/%9.812.151.564.169.983.395.2/%46.5648.579.692.488.2693.6896.1()39708PHBATP[10]2316.8107/mL22.4105/mL1/2/(−100~+50mV−100~−180mV)2.22(DPB)1(SV)4410(15%7%)10~103(7%18%)3(DPB)10~103[11]2.31565103~104/mL102~104/mL0.6319.3678%~95%76.2%25%23.3%72.5%[12][12](121~193mV)+4NH−N−3NO−N5250%42Table4ChangeofSV/actinomycesandfungiduringthesecondtypicalcycle31022405897103////////(·mL−1)332/53479/57583/563101/67489/60196/541105/531/%1579111113184SBR70951Table5Changeofnitrate/nitritebacteriaduringthefirsttypicalcycle26121620//////(·mL−1)5.7102/1.51041.610/2.51045.0102/1.71037.3103/5.71031.110/9.5103/(·mL−1)3.210/1.81031.710/2.41032.910/2.81031.710/3.11031.410/3.210361Table6Changeofammonia/nitriteandnitrateremovalefficiencyduringthefirsttypicalcycle26121620//////%78/—92/—93/—89/—95/—/mV121/−150180/−153173/−176189/−178193/−186/%76.2/—83.1/—57.4/—38.9/—25.0/—/%23.3/—15.7/—39.6/—61.0/—72.5/—[12]2.4710(1.9105/mL3.6105/mL)10(9.86%12.1%)(46.5%~50.1%)0~1010~10310351%(1.9105/mL9.6104/mL)1010312.1%48.5%95.2%96.1%32.1103/mL8.2104/mL()2///COD372(104~105/mL)(103~104/mL)23(105~107/mL)(104~105/mL)22[13]Hu[14]−3NO−NO2/−3NO−NO2Wachtmeister[15−16]2.528103−180mV()3971072Table7Changeofamountofdenitrifyingbacteria/nitrateandphosphateremovalefficiencyduringthesecondtypicalcycle31022405897103/(·mL−1)1.91053.61059.11041.91046.71045.91049.6104/%9.812.151.564.169.983.395.2/%46.5648.579.692.488.2693.6896.182Table8Changeofamountofanaerobicbacteriaduringthesecondtypicalcycle340103/(·mL−1)9.51072.51083.1108/(·mL−1)1.11053.21052.7105/(·mL−1)/mV−180−176−1863CODβ-(PHB)2.61()2()2329.8%46.56%10395.2%96.1%34595%97%12324263114911[9]9315243323323FuhsChen[10][5]9Table9MainPAOofactivatedsludgeinreactorduringdifferentphases123344433412123149113a.194275−34PO−P−3NO−NCOD12b.21015%7%10~1037%18%4SBR711c.0.6319.320.5d.100~1010351%2e.f.3[1]MeinholdJ,FilipeCDM,DaggerGT,etal.Characterizationofthedenitrifyingfractionofphosphateaccumulatingorganismsinbrologicalphosphateremovalprocess[J].WatSci-Tech,1999,39(1):31−42.[2]KubaT,vanLoosdrenchtMCM,BrandFA,etal.OccurrenceofdenitrifyingphosphorusremovingbacteriainmodifiedUCTtypewastewatertreatmentplants[J].WatRes,1997,31(4):777−786.[3]KubaT,vanLoosdrechtMCM,HeijnenJJ.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WaterResearch,l996,30(7):l702−l710.[4]AlanJ,DaidouT,TsunedaS,etal.Metabolicbehaviorofdenitrifyngphosphate-accumul