反硝化聚磷菌快速驯化的对比研究张洁

413()Vol.41No.320079　　　JOURNALOFHUAZHONGNORMALUNIVERSITY(Nat.Sci.)　　　Sept.2007:2007-02-25.:(OP060109).*E-mail:[e-mail].　:1000-1190(2007)03-0411-04反硝化聚磷菌快速驯化的对比研究张　洁*,田立江,张雁秋(,221116)　:.,,;(35d、36d),,,COD.:;;;;SBR:X131.2:A　　“”(DPB),,PHA,,,,,COD50%,30%50%[1].,DPB,,(、),,.1993DelftKuba:/,,O2NO3-,PHBA/O(PAO)[2].SBR,/,13.3%69.4%,/SBR,1mg/L,89%[2].[3],,15%73%.,:[4](-、-)(-、---、-).,,.11.11　Fig.1　ExperimentalsetupofdenitrifyingdephosphatationSBR,90cm,19.2cm,,,25L.,,,DOI:牨牥牨牴牰牥牫牤jcnki牨牥牥牥牨牨牴牥牪牥牥牱牥牫牥牪牪412　　()　　41,.,,,,150mg/L,1.,ATMEL-89S52,,,,、、、、.1　[1,5]Tab.1　Compositionofsyntheticwastewater/(mgL-1)NaAc400(COD,HAc)K2HPO45(P)NH4Cl30(N)60(CaCO3)1.22.2　Fig.2Theexperimentalprocedure1.32.2　Tab.2　TheessentialanalyzeditemsandmethodsDOYSL-52pHMLSS105℃2hTPNH4+-NNO3--NSV3030minSVI(SV30/m)2,2006325,,,MLSS5000～8000mg/L,pH6.5～7.6,8d.-,,,.,,-;,--(NaAc)--.2.13,,.3　Tab.3　Cultivatingtimeanddephosphorusefficiencybytwomethods/h/d/%(6h)(0.25h)-(3h)-(2h)-(0.5h)-(0.25h)1592(8h)(0.25h)-(3h)-(4h)-(0.5h)-(0.25h)2072(6h)(9h)(0.25h)-(3h)-(0.5h)-(0.25h)-(0.25h)-(4h)-(0.5h)-(0.25h)1181(8h)(0.25h)-(3h)-(4h)-(0.5h)-(0.25h)10792.2,,,3.　3　:413　　3　NO3--N()Fig.3　RelationshipbetweenphosphorusremovalandNO3--Nconsumption(two-phasemethod)3,,:PO43--P=1.5614NO3--N,(R2=0.9980).[6],α=5%,x1,x2,…,x6Y1,Y2,…,Y6T=β∑6i=1(xi-x)2σ*,σ*2=1n-2[∑6i=1(Yi-Y)2-β2∑6i=1(xi-x)2].T=8.06.tα2(n-2)=t0.025(4)=2.7764,Ttα2(n-2),,,.4　NO3--N()Fig.4　RelationshipbetweenphosphorusremovalandNO3--Nconsumption(three-phasemethod),:PO43--P=1.2437NO3--N,(R2=0.9788).T=19.12,Ttα2(n-2).(4),1.56141.2437,0.120.16..,.3,.,,NaAc,NaAc,()NaAc.NaAc:(1),()COD(NaAc),NO3-,,5[7].5　(a)(b)Fig.5　Apreliminarymetabolismmodelforsimultaneousphosphorusreleaseanddenitrificationunderanoxicconditions.(a)inthepresenceofavailableHAc-COD.(b)notinthepresenceofavailableHAc-COD.(2)COD,,,COD[8].4,:(1),;(2)(35d、36d),.,,.414　　()　　41:[1]　KubaT,VanloosdrechtMCM,HeijnenJJ.PhosphorusandnitrogenwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WatRes,1996,30(7):1702-1710.[2]　KubaT,VanloosdrechtMCM,HeijnenJJ.Biologicalde-phosphatationbyactivatedsludgeunderdenitrifyingcondi-tions:pHinfluenceandoccurrenceofdenitrifyongdephos-phatationinafull-scalewastewatertreatmentplant[J].WatSciTech,1997,36(12):75-82.[3]　,,,.[J].,2004,24(1):45-49.[4]　,,.[J].,2006,7(5):74-77.[5]　,.[J].,2000,22(4):44-46.[6]　.[M].:,1986:183-188.[7]　ChuangSH,OuyangCF,WangB.Kineticcompetitionbe-tweenphosphorusreleaseanddenitrificationsludgeunderan-oxiccondition[J].WatRes,1996,30(12):2967.[8]　HuJY,OngSL,NgWJ,etal.Anewmethodforcharacter-izingdenitrifyingphosphorusremovalbacteriabyusingthreedifferenttypesofelectronacceptors[J].WatRes,2003,37(14):3466.ComparativestudyontheenrichmentandcultivationofdenitrifyingphosphorusremovingbacteriaZHANGJie,TIANLijiang,ZHANGYanqiu(SchoolofEnvironmentandSpatialInformatics,ChinaUniversityofMiningandTechnology,Xuzhou,Jiangsu221116)Abstract:Theenrichmentandcultivationofdenitrifyingphosphorusremovingbacteria(DPB)bytwomethods,two-phasecultivationandthree-phasecultivation,wasinvesti-gatedinalaboratory-scaleSequencingBatchReactor(SBR).Theresultsshowed:withthesamecultivatingefficiency,thethree-phasecultivationwaspriortothetwo-phaseone;andwiththesimilarcultivatingtime(35and36days),DPBcultivatedbythree-phasecultivation,whichuptakephosphorusbyutilizingnitrateaselectronacceptor,hadhigherefficiency.Inaddition,theconclusiononthebasisofexperimentalresultswasthattheavailableCODwasaninhibitortodenitrifydephosphorus,whichwasthecruci-alreasondifferfromthetwomethods.Keywords:denitrifyingphosphorusremovingbacteria(DPB);two-phasecultivation;three-phasecultivation;two-sludgesystem;SBR