*王娟张志辉郑天龙唐丹琦汪群慧100083DNBF、HRT。36dDNBF100mg/L90%10dDNBF13d150mg/LHRT2h6h48.8%79.9%0.59kg/m3·d3.28kg/m3·d81.4%。HRTDOI10.13205/j.hjgc.201406001STUDYONTHERELATEDFACTORSOFDENITRIFICATIONBIOFILTERINSTEADYSTATEWangJuanZhangZhihuiZhengTianlongTangDanqiWangQunhuiSchoolofCivilandEnvironmentalEngineeringUniversityofScienceandTechnologyBeijingBeijing100083ChinaAbstractAtpresentdenitrificationbiofilterDNBFisgenerallyusedfortreatmentoflownitratecontaminatedwater.InthisstudytreatmentofwastewaterwithhighnitrateconcentrationbyDNBFwasresearched.TheeffectsofDNBFstartupstarvationtimehydraulicretentiontimeHRTandinletnitrateloadonnitratenitrogenremovalwereexamined.Theresultsshowedthatafter36dofstartupoftheDNBFremovalefficiencyofmorethan90%wasachievedattheinfluentNO-3-Nconcentrationof100mg/LandtheactualCODconsumptionandnitratereductionratiowas4.23Afterstarvationfor10danother13dwereneededtorestorethenitrateremovalefficiency.AttheinfluentNO-3-Nconcentrationof150mg/Landwatertemperatureof13℃whenHRTincreasedfrom2hto6hthenitrateremovalefficiencyincreasedfrom48.8%to79.9%Howeverwhenthenitrateinletloadrosefrom0.59kg/m3·dto3.28kg/m3·ditsremovalefficiencykeptbasicallystableattheaverageof81.4%.KeywordsdenitrificationbiofilterDNBFstarvationtimeHRTnitratenitrogenload*2011090242012ZX072010026。2013-09-300、1-2。DNBF3-5。DNBF、6。DNBF50mg/L3-47-8DNBF。DNBFDNBF。11.1试验装置与材料120mm×1mDNBF。63cm7.1L。30~40mm3000m2/m396%。、KNO3COD/N1WaterPollutionControl9。。1.2DNBF的启动15L、200mg/L。3。。22.1DNBF的启动性能DNBF812—926>20℃。HRT2h10mg/L100mg/LCOD/N5.0。NO-3-N1。1DNBF70%34>80%。10mg/L。DNBF。136dDNBF100mg/L95.4%。1DNBFFig.1Removalofnitratenitrogeninstartupphase。、、。110NO-3+1.08CH3OH+0.24H2CO3→0.056C5H7O2N+0.47N2+1.68H2O+HCO-31COD2。COD/N5.0CODCOD1394.0mg/L。12DNBFCOD1mgCOD4.23mg13.7COD。CODCOD。2CODFig.2ChangeinconcentrationofCODinstartupphase2.2饥饿时间对DNBF脱氮效果的影响DNBF。DNBF、、11。。DNBFDNBF。10dDNBF。3DNBFHRT2h2EnvironmentalEngineering100mg/L10d。395.6%10d。1—1060%80%。13dDNBF98.9%。DNBF。310dDNBFFig.3Recoveryofnitratenitrogenremovalafterstarvationfor10d2.3HRT对DNBF脱氮效果的影响COD/N4.0150mg/LHRT246hDNBF。112311011~15℃13.3℃。HRTDNBF4。HRT2h43.8%。HRT4h6h68.1%79.6%。HRTDNBF。HRT。6hHRT。2.4硝氮负荷对DNBF脱氮效果的影响HRT6hDNBF。17~23℃21.4℃。5DNBF。81.4%。3.28kg/m3·d85.7%2.81kg/m3·d。54HRTDNBFFig.4EffectofHRTonnitratenitrogenremoval3.28kg/m3·dR20.985。5DNBFFig.5Changeinremovalrateandefficiencyofdifferentnitratenitrogeninletload3136dDNBF100mg/L90%。2HRTDNBF。10dDNBF13d。13℃HRT2h6h48.4%79.6%。3。0.59kg/m3·d3.28kg/m3·d81.4%。1SavciS.InvestigationofeffectofchemicalfertilizersonenvironmentJ.ApcbeeProcedia20121287-292.2.J.2003244109-112.73WaterPollutionControl。。。1.J.2012244379-381.2.J.200915764-66.3.J.199616166-72.4.J.200830784-87.5.J.201130687-91.6.J.20041749-10.7.J.2010271069-72.8.J.200826S113-15.9BaljitKaurManjuSharmaRohitSonietal.Proteome-basedprofilingofhypercellulase-producingstrainsdevelopedthroughinterspecificprotoplastfusionbetweenAspergillusnidulansandAspergillustubingensisJ.AppliedBiochemistryandBiotechnology20131692393-407.10SivakumarUKalaichelvanGRamasamyK.ProtoplastfusioninStreptomycessp.forincreasedproductionoflaccaseandassociatedligninolyticenzymesJ.WorldJournalofMicrobiology&Biotechnology2004206563-568.11WeiWLWuKLQinYetal.IntergenericprotoplastfusionbetweenKluyveromycesandSaccharomycescerevisiae-toproducesorbitolfromJerusalemartichokesJ.BiotechnologyLetters20012310799-803.12JinZHXuBLinSZetal.EnhancedproductionofspinosadinSaccharopolysporaspinosabygenomeshufflingJ.AppliedBiochemistryandBiotechnology20091593655-663.13StemmerWPC.MolecularbreedingofgenespathwayandgenomesbyDNAshufflingJ.JournalofMolecularCatalysisBEnzymatic20021923-12.14ManojKumarMahendraPratapSinghDeepakKumarTuli.GenomeshufflingofPseudomonassp.Ioca11forimprovingdegradationofpolycyclicaromatichydrocarbonsJ.AdvancesinMicrobiology2012226-30.15ChenZWenJSongYetal.EnhancementandselectiveproductionofavermectinBbyrecombinantsofStreptomycesavermitilisviaintraspecificprotoplastfusionJ.ChineseScienceBulletin2007525616-622.16XuJZZhangJLZhangWGetal.ThenovelroleoffungalintracellularlaccaseusedtoscreenhybridsbetweenHypsizigusmarmoreusandClitocybemaximabyprotoplasmicfusionJ.WorldJournalofMicrobiology&Biotechnology20122882625-2633.17.J.201117481-83.1974-。zhaolh05@163.com1987-。櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅櫅wenzily@126.com33.J.2008242126-29.4.J.201081615-1621.5RyuHDKimDLimHEetal.Nitrogenremovalfromlowcarbon-to-nitrogenwastewaterinfour-stagebiologicalaeratedfiltersystemJ.ProcessBiochemistry2008437729-735.6.J.200935-7.7DongWYZhangXBWangHJetal.EnhanceddenitrificationwithexternalcarbonsourcesinabiologicalanoxicfilterJ.WaterScienceandTechnology201266102243.8AslanSCakiciH.BiologicaldenitrificationofdrinkingwaterinaslowsandfilterJ.JournalofHazardousMaterials20071481253-258.9SakumaTJinsiriwanitSHattoriTetal.Removalofammoniafromcontaminatedairinabiotricklingfilter-denitrifyingbioreactorcombinationsystemJ.WaterResearch200842174507-4513.10McCarthyPLBeckLAmantPS.BiologicalDenitrificationofWastewatersbyAdditionofOrganicMaterialsC.24thIndWasteConf1969.11HwangKSongMKimWetal.Effectsofp