反硝化生物膜启动厌氧氨氧化反应器的研究

:025322468(2004)0220220205:X703:A,3,(,310029):,,,,Anammox.3,Anammox01143kgm-3d-1,86152%,NH+42NNO-22N1mgL-1.NH+42NNO-22NNO-32N,Anammox.:;;Start2upofAnammoxbioreactorwithdenitrificationbiofilmZHANGShaohui,ZHENGPing3,HUAYumei(Dept.ofEnvironmentalEngineering,ZhejiangUniversity,Hangzhou310029)Abstract:Theanaerobicammoniaoxidation(Anammox)bioreactorwassuccessfullysetupwiththedenitrificationbiofilm.Afterathree2monthoperation,thevolumetrictotalnitrogenloadingratewas01143kgm-3d-1andthetotalnitrogenremovalwas86152%witheffluentammoniaandnitritelessthan1mgL-1.TheratioofNH+42Nremoval,NO-22NremovalandNO-32Nproductionaswellasthebiofilmcolorcouldindi2catethedevelopmentofAnammoxbioreactorperformance.Keywords:denitrificationbiofilm;Anammoxbioreactor;start2up:2003205219;:2003209228:(30070017);(500014);:(1972),,;3:[e-mail](Anaerobicammoniaoxidation,Anammox)2090,,,[13],.,,11d[4].,,,Anammox.,,,Anammox[5,6].,,.Anammox?,Anammox.1111,MLSS86104gL-1,MLVSS23156gL-1,MLVSSPMLSS27138%.112:KH2PO430mgL-1,KHCO3500mgL-1,MgSO4200mgL-1,FeCl324220043ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.2Mar.,2004100mgL-1,CaCl230mgL-1,NaNO3CH3COONa.:KHCO3500mgL-1,KH2PO42712mgL-1,MgSO47H2O300mgL-1,CaCl2136mgL-1,1mLL-1,NaNO2(NH4)2SO4.:EDTA5000mgL-1,FeSO45000mgL-1;:EDTA15000mgL-1,ZnSO47H2O430mgL-1,CoCl26H2O240mgL-1,MnCl24H2O990mgL-1,CuSO45H2O250mgL-1,Na2MoO42H2O220mgL-1,NiCl26H2O190mgL-1,Na2SeO410H2O210mgL-1,H3BO414mgL-1.1131Fig.1Schematicdiagramofexperimentalequipment,115L,40cm.,20h,.1.2830.114[7,8]COD:;NO-32N:,;NO-22N:N2(12)2;NH+42N:2;:2;:MPN.2211,r,();K,().,,,.:5CH3COO-+8NO-34N2+10CO2+H2O+13OH-(1),(HydraulicRetentionTime,HRT)15h,NO-32N14119mgL-1,CPN313316,NO-32N,NO-32NCOD,[9].CPN313316,NO-32N2mgL-1,35mgL-1NO-32N350mgL-1(2),.,.,HRT,.,NO-32N.3,HRT6h,NO-32NNO-22N1mgL-1.,,.60d,MPN10101011mL-1.1222:2HRT=15hFig.2ProfileofdenitrificationatHRT=15h3HRTFig.3ComparisonofdenitrificationatdifferentHRT212Anammox,,,[10,11].,,,.1d,An2ammox.,NH+42NNO-22N70mgL-1,HRT24h,pHNaHCO3615815.4Fig.4Variationinsubstratesduringinitialstageofstart2up212114,,16dNO-22N,NH+42N,NH+42N.,[10],MPN108109mL-1,.NH+42NNO-22N,Anammox,NH+42NNO-22N,.,.53d,NH+42NNO-22N37172%4127%,NH+42NNO-22N.,[5],MPN107mL-1.,[12],,HRT48h.,pH(9),.21212pH716812,HRT24h,5.415mgL-1,,3dNH+42NNO-22N.4d,NO-22N,NH+42N(37174%).22d,NH+42NNO-22N1,41166%222244315%.NH+42NNO-22N,111111,.36d,Anammox01143kgm-3d-1,86152%,NH+42NNO-22N1mgL-1.NH+42NNO-22NNO-32N:110122,(111320126)[13].,Anammox.,,,,.5Fig.5Variationofsubstratesduringincreaseofactivity63Fig.6VariationinRatiosofthreekindsofnitrogencompoundsduringincreaseofactivity,,015%,[12].,,415mgL-1,,.,().NH+42NNO-22N,NH+42NNO-22N(11)(11132).6,,NH+42NNO-22NNO-32N.NH+42NNO-22N11,.213,,.,.,.Anammoxc[3],.3(1),,,,Anammox.3,01143kgm-3d-1.3222:(2),,Anam2mox.(3),NH+42NNO-22NNO-32N,,.:[1]MulderA,VandeGraafAA,RobertsonA,etal.Anaerobicammoniumoxidationdiscoveredinadenitrifyingfluidizedbedreactor[J].FEMSMicrobiolEcol,1995,16:177183[2]VandeGraafAA,MulderA,DeBruijnP,etal.Anaerobicoxidationofammoniumisabiologicallymediatedprocess[J].ApplEnvionMicrobiol,1995,61(4):12461251[3]VandeGraafAA,DeBruijnP,RobertsonLA,etal.Autotrophlicgrowthofanaerobicammonium2oxidizingmicro2organismsinafluidizedbedreactor[J].Microbiology,1996,142(8):21872196[4]StrousM,HeijnenJJ,KuenenJG,etal.Thesequencingbatchreactorasapowerfultoolforthestudyofslowlygrowinganaerobicammonium2oxidizingmicroorganisms[J].ApplMicrobiolBiotechnol,1998,50(27):589596[5].[D].:,2000[6],,,.[J].,2002,18(4):492496[7].()[M].:,1989[8],.()[M].:,1985:8084,227238[9],.[J].,2003,29(1):3234[10]CervantesFJ,DelaRosaDA,GomezJ.NitrogenremovalfromwastewatersatlowCPNratioswithammoniumandacetateaselec2trondonors[J].BioresourceTechnology,2001,79(2):165170[11]CervantesFJ,MonroyO,GomezJ.Influenceofammoniumontheperformanceofadenitrifyingcultureunderheterotrophiccondi2tions[J].ApplBiochemBiotechnol,1999,81(1):1323[12]StrousM,VanGervenE,KuenenJG,etal.Effectsofaerobicandmicroaerobicconditionsonanaerobicammonium2oxidizing(An2namox)sludge[J].ApplEnvironMicrobiol,1997,63(6):24462448[13]StrousM,KuenenJG,JettenMSM.Keyphysiologyofanaerobicammoniumoxidation[J].ApplEnvironMicrobiol,1999,65(7):32483250[14]SchmidtI,SliekersO,SchmidM,etal.Aerobicandanaerobicammoniaoxidizingbacteria2competitorsornaturalpartners?[J].FEMSMicrobiologyEcology,2002,39(3):17518142224