反硝化微生物在污水脱氮中的研究及应用进展肖晶晶

12*1.1000812.100081、、。N2。。、、。X172Adoi:10.3969/j.issn.1003-6504.2009.12.0221003-6504(2009)12-0097-06ApplicationofDenitrifyingMicrobestoWastewaterDenitrificationXIAOJing-jingGUOPingHUOWei-jieYUJiangZHUChang-xiong*1.InstituteofAgriculturalEnvironmentandSustainableDevelopmentCAASBeijing100081China2.KeyLaboratoryforAgro-environmentandClimateChangeMinistryofAgricultureBeijing100081ChinaAbstractAgriculturalwastewaterincludingpesticideandfertilizeraswellasaquaculturewastewaterandsewagecanresultinnitrogenpollutionofwater.Denitrifyingmicrobeswhichhaveanimportanteffectondenitrogenationofwastewatercanturnnitrite-nitrogenornitrate-nitrogenintoN2inordertoreducenitrogencontentinwastewaterandimprovewaterquality.Mechanismofdenitrifyingmicrobesintermsoffunctiontypicalstrainandenzymeswerereviewedtheapplicationprogresswereintroduced.Someexistingproblemandfuturedevelopmenttrendwerealsoproposed.Keywordsdenitrifyingmicrobesfunctiontypicalstraindenitrifyingenzymesexistingproblem。NO、N2ON2。。。1。、。、、。、、、、、。。、。250130。Pseudomonaceae、AlcaligenesNeisseriaceae、Nitrobacter－aceae、Rhodospirillaceae、Bacillaceae、Cytophagaceae、Spirillaceaee、Rhizobiaceae、2008-08-012009－01[e-mail]*[e-mail]。EnvironmentalScience&Technology3212200912Vol.32No.12Dec.200932Halobacteriaceae。。。、[1-3]Fusarium、Gibberella、Trichoderma、Cylindrocarpon、Chaetomium、Penicillium、AspergillusHansenula。[4]StreptomycesFrankia。。2.1。。。RobertsonKuenenaerobicdenitrification。2080Robertson[5]ThiosphaerapantotrophaParacoccusdenitrifications[6]O2NO3-。[7-12]bacillus、Alcaligenes、Paracoccus、Klebsiella、Ochrobactrum、Zoogloea、Pseudomonas、HyphomicrobiumX、Agrobacterium、Eubacterium、Propionibacterium、Blasto－bacter、HalobacteriumRhi－zobium。Pochana[13]SBR95%。Frette[14]/16。。2004[15]RhodococcusStenotrophomonasmaltophilia10h26.18mg/L0[16]。。1。-pH、、、。1Table1Typicalandnewdenitrifyingmicrobesanditsnitrogenremoval(DO)(mg/L)DNF409Bacillussp.bNO3-43.20N2O0.683a[25]DNF409Bacillussp.bNO2-6.04N2O0.85a[25]WXZ-4Pseudomonas5aNO3-35-0.255a[26]WXZ-9Delftia5aNO3-35-0.218a[26]D3PseudomonasmendocinadNO3-88.5-26.2a[27]C3Pseudomonas8~10aNO3-110.98N212.07a[28]A.denitrificansDCBT25Alcaligenesdenitrifica/piechaudiicNO3-1386N20.092c[29]K.pneumoniaeF-5-2Klebsiella-NO3-700N214.56a[30]O.anthroptDCBT23OchrobactrumanthropicNO3-1386N20.072c[29]MicrovigulaaerodenitrificansMicrovirgulaaerodenitrificans1.60aNO334.7N21.47a[31]Paracoccus.spDB2Paracoccus7.50aNO3-250N20.128b[32]P.immobilisDCBT6PsychrobacterimmobiliscNO3-625N20.164c[29]Z.strainTL1Zoogloea0.75aNO3-554N20.960b[33]X0412Stenotrophomonasmaltophilia3.80~5.21aNO2-26.18-2.618a[16]W2Bacillussp.4.9aNO2-700N2O,N226.8a[34]1O2a.DOmg/Lb.c.d.。2a.mg/L·hb.mmolN/gprotein·hc.mmolN/gdryweight·h。3“-”。9812。2.2CO2HCO3-[17]Thiobacillusdenitrificans、Thiomicrospiradenitrificans、Nitrosomonas、Branhamellacatarrhalis、Gemisoceanospirllum。Vidal[18]。Koenig[19]。Sliekers[20]。C/N。。。2.3DenitrifyingPhosphateAccumu－latingOrganismsDNPAOs。。[21]。2006[22]、PHBH6、H19、H24Xg。Pseu－domonasEnterobacter。2.4Pseudomonasfluorescens、Pseu－domonasaeruginosa、Alcaligenesface－calis、Pseudomonasaureofaciens、Pseudomonasaeruginosa。AnshumanA.[23]8Diaphorobacte。2.5、。[24]8℃H16Pseudomonas。Haloarcula、Halobacterium、Haloferax、HalomonasBacillus。。32080。1。1。、1。4N2ON2。T.pantotrophoNO3-、NO2-、NO、N2ON2[35]。、、。2。5DPDenitrificationPotentialDEADenitrifyingEnzymeActivity、、。DPN2ON2。。DEA。9932。。DP。DEA。[38-42]。1C2H2。C2H2C2H2N2ON2N2O。、、。NO3-C2H2N2ON2C2H2C2H2/2N2。/。N2N2315NIPT15N、NO3-NH4+、N15NO3-14NO3-15NO3-14NO315NO3-。4MIMS515N-NO3-6NO3-7N89O2/N2O。C2H2。6[43]3%。。RFLPDGGEnirK、nirS、nosZPCRPCRGC-FAME、FISH16SrRNA。7、。。。。1。2N2ON2。N2O2、、、、[36-37]Table2ThenametypesiteexpressionconfigurationandcodinggenesofDenitrifyingenzymes()。NapANapB。NapAnapA,MGD()[4Fe-4S]。NapBC552。(),。3α()。αnarG。βnarH4[4Fe-4S]。γnarI2b。(cd1)nirs。2(His-17,His-69)c,(Tyr-25)(His-200)d1。c,d1O2。Cu。nirk。N2OpHN2O。bc,2,NORBb,NORCcnorCB。。NONONO。NON2O。。N2。pH。6Cu,2,CuA,CuZ。Cytc554。10012N2O。3、。、、。4、、。5、。、。。、、、、。。、。[][1]BleakleyBHTiedjeJM.Nitrousoxideproductionbyor－ganismsotherthannitrifiersordenitriers[J].ApplEnviron.Microbiol1982441342-1348.[2]BllagJMTungG.Nitrousoxidereleasebysoilfungi[J].SoilBiochem19724271-276.[3]ShounHKimDHUchiyamaHetal.Denitrificationbyfungi[J].FEMSMicrobiolLett199294277-281.[4]ShounHKanoM.DenitrificationbyactinomycetesandpurificationofdissimiliatorynitritereductaseandazurinfromStreptomysthioluteuces[J].JBacteriol19981804413-4415.[5]RobertsonLAKuenenJG.Aerobicdenitrificationacon－troversyrevived[J].ArchMicrobiol1984139351-354.[6]LukowTDiekmannH.AerobicdenitrificationbyanewlyisolatedheterotrophicbacteriumstrainTL1[J].Biotechnologyletters199711191157-1159.[7]LaurentPAlainHCatherineHetal.16SrDNAanalysisforcharacterizationofdenitrifyingbacteriaisolatedfromthreeagriculturalsoils[J].FEMSMicrobiologyEcology200034121-128.[8]LonisASMajaVV.Fiveyearsofnitriteremovaldenitri－ficaionandcarbondynamiesinadenitrificationwall[J].WatRes200135143473-3477.[9]PatureauDZumsteinEDelgenesJPetal.Aerobicdeni－trificationisolationfromdiverse