反硝化细菌在污水脱氮中的作用辛明秀

辛明秀1＊　赵　颖2　周　军2　高文臣1(100875)1　(100038)2:,。、、。,、、。,、。:,,:Q939.9　　:A　　:0253-2654(2007)04-0773-04TheApplicationofDenitrifyingBacteriainDenitrificationofWastewaterXINMing-Xiu1＊,ZHAOYing2,ZHOUJun2,GAOWen-Chen1(CollegeofLifeSciences,BeijingNormalUniversity,Beijing100875)1(BeijingDrainageGroupCo.Ltd(BDG),Beijing100038)2Abstract:Denitrificationplaysanimportantroleinwastewatertreatmentsystemsforthereasonthatthenitrateornitritewasreduced,andsomegasessuchasNO,N2OorN2werereleased.Theapplicationofdenitrificationinwastewater,themechanismofdenitrification,andtheeffectfactorsofdenitrificationwereintroducedinthispaper.ThecommunitiesofDenitrifyingbacteria,andsomekeyenzymesofdenitrificationwerealsointroduced.Thediscoveryofaerobicdenitrifyingbacteria,autotrophicdenitrifyingbacteria,anddenitrifyingPhosphorus-removingBacteriawerealsomentionedinthispaper.Keywords:Denitrification,Denitrifyingbacteria,Denitrificationofwastewater　　＊　Tel:[phone],E-mail:[e-mail]:2006-10-11,:2007-03-28　　(Denitrification),,NO、N2O,N2。。,,NO、N2O,N2,。(Denitrifyingbacteria),,、。,[1]。1　,,。,,。,(DO)0.5mgL,(BOD),,NO、N2ON2,,pH,。10%～90%。。,、Na2S·773·　200734(4)DOI:10.13344/j.microbiol.china.2007.04.040,,,NH3。。,50,130。(Achromobacter),(Bacillus),(Brevibacterium),(Enterbacter),(Lactobacillus),(Micrococcus),(Pseudomonas),(Spirillum)。,。。。,Hoshino[2]。,[3]。16SrDNA,,,[4]。(napAnarG)、cd1Cu(nirSnirK)(nosZ)。2　2.1　,BOD5T-N3～5,,。,CO2H2O,。。2.2　pHpH6.5～7.5,pH。pH6.08.0,。NO-3NO-2pH。2.3　25℃～35℃,15℃,。15℃～30℃。2.4　,。,。,。,0.5mgL[5]。,、,,[6]。3　[7]。。。,(NO-2)NO,cd1Cu-。NON2ObcNO,N2ON2,CuN2O。。,NON2O。3.1　,,。(Pseudomonasaeruginosa),(Paracoccusdenitrificans),(Pseudomonasstutzeri),(P.fluorescens),(E.coli)。3,α,。αnarG。β,narH·774·200734(4),4[4Fe-4S]。γ,narI,2b。2,αβ,γ,γαβ,γ,γβ(b-)。2,NapANapB[8]。,[4Fe-4S]。NapBC-,NapC,。nap,(Ralstoniaeutropha),(Paracoccusdenitrificans)。、。,NADPH,。NADPH,。(Paracoccusdenitrificans),,,,。3.2　[9]。[10],。(NirSNirK),,。,。NirS,,30%NirK。NirSNirK。nirKnirS(Proteobacteria)。SARAHALLINnirKnirSPCR。XueduanLiuPCRnir。nir[11]。。4　,。(Paracoccuspantotrophus),N2。(Pseudomonasstutzer),Paracoccuspantotrophus。。Paracoccuspantotrophus,。N2ON2。NaokiTakaya,[12]。,[13]。5　[14](CO2,HCO3-),,。,,。,。(Thiobacillusdenitrificans)(Thiomicrospiradenitrificans)。6　,,,。[15]。(DenitrifyingPhosphorus-removingBacteria)。,。·775·　200734(4),。7　7.1　,。,。,,。7.2　,。。2004、,。7.3　,,,。,16SrDNA。,,5℃～10℃()。7.4　,,。,、,。、,,。,,,。[1]DongYL,AdelaR,LeeM,etal.ApplEnvironMicrobiol,2002,68(5):2140～2147.[2]HoshinoT,TeraharaT,TsunedaS,etal.JofApplMicrobiol,2005,99:1165～1175.[3]BinbinLiu,FengZhang,XiaoxiFeng,etal.FEMSMicrobiolEcol,2006,55:274～286.[4]GescheB,JizhongZ,LiyouIW,etal.ApplEnvironMicrobiol,2000,66:2096～2104.[5]McKenneyDJ,DruryCF,WangSW.SoilSciSocAMJ,2001,65:126～132.[6]YoshieS,OgawaT,MakinoH,etal.LettersinApplMicrobiology,2006,42:277～283.[7]WalterGZumft.MicroandMolBiologyReview,1997,61(4):533～616.[8]LauraB,TaoW,RickWYE.JofBacteriology,1999,181(9):2802～2806.[9]HubertusJEB,NormanGH,LuisAS-S,etal.JofBacteriology,2002,184(9):2557～2560.[10]GescheB,JizhongZ,LiyouW,etal.ApplEnvironMicrobiol,2000,66(5):2096～2104.[11],　,,.C,2005,35(3):246～253.[12]NaokiT,MariaAB,CatalanS,etal.ApplEnvironMicrobiol,2003,69:3152～3157.[13]QinXK,XinWW,MinJ,etal.FEMSMicrobiolLett,2006,260:150～155.[14],,,2005,25(2):88～92.[15]YoramB,JaapV.ApplEnvironMicrobiol,2000,66:1209～1212.·776·200734(4)